EMAGE mouse gene expression spatial database

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EMAGE:11914

Sec24d Sec24 related gene family, member D (S. cerevisiae) ( MGI:1916858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010979. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010979_01	euxassay_010979_02	euxassay_010979_03	euxassay_010979_04

euxassay_010979_05	euxassay_010979_06	euxassay_010979_07	euxassay_010979_08	euxassay_010979_09

euxassay_010979_10	euxassay_010979_11	euxassay_010979_12	euxassay_010979_13	euxassay_010979_14

euxassay_010979_15	euxassay_010979_16	euxassay_010979_17	euxassay_010979_18	euxassay_010979_19

euxassay_010979_20	euxassay_010979_21	euxassay_010979_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11914_wholemount_strong.wlz

11914_wholemount_moderate.wlz

11914_wholemount_weak.wlz

11914_wholemount_possible.wlz

11914_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11914_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9316
Entity Detected:	Sec24d, Sec24 related gene family, member D (S. cerevisiae) ( MGI:1916858)
Sequence:	sense strand is shown >T9316 ACTCGGCGGCGGCGGCGCGGGGCGCGGAGCGTGCAGAGCAGGGTTATTCTGCAGTAGCTCTTCTTTGGAA TGATATTTTCATCATGAGCCAACAAGGCTATGTGGCGACACCCCCTTATTCTCAGTCCCAGCCTGGAATG GGTATCTCTCCCCCTCACTACGGACACTATGGGGACCCGTCCCATGCATCCTCTCCACCAGGCGTGATGA AGCCATTGGGGCCTTCAGCTGCTCCTTCAGGGATGCTGCCCCCAGGTCCTTTACCTCCTGGACCCCCACA GTTTGGCCCCAATGGAGCCCATACTCCAGGCCATCCTCCTCAAAGATTTCCAGGACCCCCACCTGTCAAT AGTGTGGCACCCTCTTATGCGTCAGGCCAGACCCTGCCACAGTCATCTTACCCAGGCCCTGGGTCCACTT CCTCTGTCACCCAGCTGGGCAGTCAGTTCAGCTCCATGCAGATCAACAGCTATGGTGTGGGCGCGACTCC TCAGAGCCAGGGTCCGCCTGGCCCCCAGTCAGCAGGCGCCTTCCAGGGGCCTCCACAACCTGCACAGCCA TCTATTCTGCAGCCAGGACATCAGGTTCCGCCGCCACCCCCCACTGCCCTGAACGGCCCTGGTGCCTCTC CTATGTCTCCACCAACTCACAGGCAAGATGGCCTTCCTGGGCCAGCTCCTCTGAACGCCCAGTATCAGCC CCCACCTCCACCAGGCCAGACCCTGGGTCCTGGCTACCCTCCACAGCAAGCAACCAACTATGGACCGCAG ATGGGAGGTGCACAGATGTCTTACCCAGGAGGGTTCCCTGGAGGTCCTGCCCAGATGGCTGGGCCGGCAC CACAGCTCCAGAGGAAGCTGGATCCGGACTCCATCCCCAGCCCTATTCAAGTGATCGAGAATGACAGGGC CACCAGAGGTGGACAAGTCTATACGACCAATACCAGGGGCCAGGTACCTCCACTGGTCACCACCGAGTGT GTGATTCAAGACCAAGGCCACTCGAGCCCCCGCTATATCCGCTGCACTACGTATTGCTTTCCGTGCACGT CCGACATGGCCAAGCAAGCTCAGATCCCACTGGCTGCCGTCATCAAACCCTTCGCTGACATTCCGCCCAA CGAGACCCCCTTGTACTTGGTAAACCATGGGGAGAGTGGACCAGTGAGGTGCAACAGGTGCAAAGCCTAC ATGTGCCCGTTCATGCAATTCATCGAAGGCGGACGGCGCTATCAGTGCGGATTCTGCAGCTGTGTGAATG AAGTCCCACCATTCTATTTCCAACATCTGGACCACATTGGAAGGAGATTGGACCACTATGAGAAGCCAGA GCTGTCGCTGGGATCCTATGAATATGTTGCTACATTGGATTACTGCAGAAAGAATAAACCTCCCAGCCCA CCAGCCTTCATCTTCATGATTGATGTCTCATATAGTAACATAAAGAATGGCCTCGTCAAGCTCATATGTG AAGAGCTGAAGACCGCGCTGAAGCGGCTACCCAAGGAGGAGCATGAGGAGACGTCTGCAATCCGAGTTGG TTTCATCACATACAACAAAGTTCTCCACTTCTTTAACGTGAAGAGTAATCTGGCCCAGCCTCAGATGATG GTGGTGACGGATGTCGGAGAGGTCTTTGTTCCTTTGTTGGATGGGTTCCTTGTCAACTATGAAGAATCTC AATCTGTGATCCACAATTTATTGGACCAGATTCCTGAGATGTTTGCTGACTCTAATGAAAACGAGACAGT CTTTGCCCCGGTCATCCAGGCCGGCATGGAGGCCCTGAAGGCAGCAGAGTGCCCGGGGAAGCTCTTCATC TTCCATTCCTCTTTGCCTACTGCCGAAGCACCGGGGAAGCTCAAGAACAGAGACGACAAAAAGCTGGTGA ACACAGACAAAGAGAAGATACTTTTCCAGCCCCAAACAGCTGTCTACGAATCTCTGGCCAAAGACTGTGT GGCCAACAGCTGCTCAGTGACTCTGTTCCTCTTCCCCAGTCAGTTTGTGGATGTGGCTTCCTTGGGTCTG GTTCCTCTGCTCACGGGAGGAACTCTTTACAAATACAACGTTTTCCAGATACATTCAGATAGCCAAAGGT TTCTGACCGACCTCCGGAATGATATTGAGAAGAAAATAGGCTTCGATGCTATCATGAGGGTTCGGACCAG CACAGGTTTCAGGGCCACTGATTTCTTTGGTGGAATATTCATGAACAACACCACAGATGTGGAAATGGCG GCCATCGACTGCGATAAAGCCGTGACAGTGGAGTTCAAGCACGATGACAAGCTCAGTGAAGATGTCGGGG CCCTGATCCAGTGTGCTGTGCTCTACACGACCATCAGTGGTCAGCGAAGACTCCGGATTCACAATCTCGC CTTAAACTGCAGCACCCAGCTAGCGGATCTGTACAAGAGCTGTGAAACTGATGCCCTTATCAACTTCTTT GCCAAGTCAGCTTTTAAAGCAGTTTTGAACCAGCCCTTGAAGGCCATCCGGGAAATTCTAGTCAATCAGA CTGCCCATATGCTGGCGTGTTACCGGAAGCACTGTGCGAGTCCGTCTGCAGCAAGCCAGCTTATACTGCC AGATTCCATGAAGGTGCTGCCTGTGTACATGAACAGTTTGTTGAAAAACTGTGTGCTGCTCAGCAGATCG GAGATCTCCCCTGATGAGCGGGCTTACCAGCGGCAGCTGGTCATGACCATGGGCGTGGCTGACTCCCAGC TCTTCTTCTATCCACTGCTCCTGCCAATCCACACGTTAGATGTGAAGAGTGCAGCGCTGCCACCAGCCGT CCGCTGCTCCGAGTCCCGACTCTCTGAAGAAGGAATATTCCTGCTGGCTAACGGTCTGAACATGTTCTTA TGGTTTGGAGTGGGCAGCCCACCAGAGCTGATTCAGGGAATATTTAATGTGCCATCGTTTGCACATATCA ACACAGATATGACATCGCTGCCTGAGGTGGGAAGTCCACACTCTCAACAACTCAGAATGATAATGAATAA TATCCAGCAAAAGAAGCCATACTCAATGAAGCTCATCGTGGTTAAACAGCGAGAGCAGCGGGAGATGGCT TTCCGACAGTTCCTGGTGGAAGATAAAGGGCTCTATGGAGGATCTTCGTACGTGGATTTCCTCTGCTGTG TTCACAAGGAGATCTGTCAACTGCTTAACTGAGCCCTCGGCTCTCCCTCTGAGGTCACATTCCTGAGGAG ACAGTCTCCAACTTGGTGCCTAATTTTCTGATGATAGTAGGCTAGTTTTAATTTCTTGATCACTTTCAGG ACAGCTTGCCTGGACAGCACACAGACCTTCAGCTCGGAGTGCTCAGGTGGCAAGCCCGTGGAGGTGTGGG GGTACTGTAAAGGGAAGAGTCCATTACTGGCTGTCCAGCTTCTAATTTCTAAAGCTGACACAATTTGCAT TTCATAAATACATAGTTTACAGAACCATTTGCAGCCTCAATTTTCTTTGTGCTTGTTATATAAATTATTT TTCAAACTGTATAGATTCATGATAAAATTATCTTGGTTCCTCTCTCTTTTACCGTGAGAGGAAAAGACTT CATTTTTATCTTATACCTAATTTTTTTAAAACCCCGTGATGACATTAAACAGAATTTTTTTTCCAACATA AGATCTGCACAGCAGACTTTTAAAAAGCCTGAAATCTGTCTGGTAGAACAATTTGTGTTCTCCTTTTTTC ATAATTATATATTGTCTGTTTAAAATATTTTCCAGTTGTTTTGTCTATAAAACGGTATCTATATTCTTAA TGGTTCTTTGTACAATTTTGATGGTTTCTACCTGTATATAATGGATCTTAACCAATATCAATAAATTACT TCATGTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6847085 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6847085 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827936 same experiment
	EurExpress:euxassay_010979 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source