EMAGE mouse gene expression spatial database

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EMAGE:11929

Nckap5 NCK-associated protein 5 ( MGI:2686394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008577. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008577_01	euxassay_008577_02	euxassay_008577_03	euxassay_008577_04

euxassay_008577_05	euxassay_008577_06	euxassay_008577_07	euxassay_008577_08	euxassay_008577_09

euxassay_008577_10	euxassay_008577_11	euxassay_008577_12	euxassay_008577_13	euxassay_008577_14

euxassay_008577_15	euxassay_008577_16	euxassay_008577_17	euxassay_008577_18	euxassay_008577_19

euxassay_008577_20	euxassay_008577_21	euxassay_008577_22	euxassay_008577_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11929_wholemount_strong.wlz

11929_wholemount_moderate.wlz

11929_wholemount_weak.wlz

11929_wholemount_possible.wlz

11929_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11929_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

homogeneous

weak expression: see section 12 13 14

diencephalon lateral wall ventricular layer

weak

homogeneous

weak expression: see section 12 13 14

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 10 11 15 16 17

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

weak

homogeneous

weak expression: see section 10 11 12 14 15 16 17

rest of cerebellum ventricular layer

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

pons ventricular layer

weak

homogeneous

weak expression: see section 09 10 11 12 14 15

midbrain ventricular layer

weak

homogeneous

weak expression: see section 10 11 12 13 14 15 16

spinal cord ventricular layer

weak

homogeneous

weak expression: see section 14 15

lung

moderate

regional

moderate expression: see section 05 06 07 09 10 11 12 13 14 15 17 18 19 20 21 22 23 weak expression: see section 08 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36234
Entity Detected:	Nckap5, NCK-associated protein 5 ( MGI:2686394)
Sequence:	sense strand is shown >T36234 GAACAGGGGGTGTGGTTAAATATAGCTCCTCCTACTGGAACTCCAGAACTGCCTATGCACTTAGGCGTGT AGGAGCGCCGTGCTTCCAAAACTGCCAGTCCTGGTCCCAGGAGGGCTCACATTGTACACACAGTTGGAAA CAGGGACAGGTTTCTGAATATTCAGTGGCCAGAAATCAGTTCAATATGAAGCGGATGATGATTTTTCTGG CACTTCTGGCAGCATGCCCCTATGCTGTAGGCTTTGTTCTGAATACACTGCATACTTCCCAGTATGTCAA GCTATGCAGTGTTGCCATGCAGAATCAGGTCCCAGCGCAGTGTGCCTGTTAGAAGTTACGGTGTCTTTAT TGCACATTACATCTTATAATCTTAGAGAAACATGGTGGGTGGTTTTTTTTTTTTTTCATGTTCTCCAAGA ACAAAAGTGTTTAATAGTTTCCTATTTCCCACGGCATGAAAGCAACAAATTAATATATATCTAGACTGTG TCATATTAGAATGTCTCGCTTTTAGATTGCTGTTCAAGTTTCTAACTTGAGTTCCATAAAATAATCTGCT AGATGAATTGTGGCATGTGATTTGGACAGAAATTTCAGCTGTTTCTCTAATAACACGATACATACGTCTG ACTTTTTGAACTATGAATTTATATGAATATATAATGCCATTCCCCAAATTGACAGTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69127. Forward Primer - name:069127_F_cDNA_D130011D22Rik, sequence:GAACAGGGGGTGTGGTTAAATA; Reverse Primer - name:069127_N_SP6_cDNA_D130011D22Rik, sequence:AACTGTCAATTTGGGGAATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11931 same embryo
	EMAGE:11932 same embryo
	EMAGE:11930 same embryo
	EMAGE:11934 same embryo
	EMAGE:11933 same embryo
	EurExpress:euxassay_008577 same experiment
	MGI:4826620 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source