EMAGE mouse gene expression spatial database

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EMAGE:11955

Insr insulin receptor ( MGI:96575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011041. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011041_01	euxassay_011041_02	euxassay_011041_03	euxassay_011041_04

euxassay_011041_05	euxassay_011041_06	euxassay_011041_07	euxassay_011041_08	euxassay_011041_09

euxassay_011041_10	euxassay_011041_11	euxassay_011041_12	euxassay_011041_13	euxassay_011041_14

euxassay_011041_15	euxassay_011041_16	euxassay_011041_17	euxassay_011041_18	euxassay_011041_19

euxassay_011041_20	euxassay_011041_21	euxassay_011041_22	euxassay_011041_23	euxassay_011041_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11955_wholemount_strong.wlz

11955_wholemount_moderate.wlz

11955_wholemount_weak.wlz

11955_wholemount_possible.wlz

11955_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11955_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 09 10 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36514
Entity Detected:	Insr, insulin receptor ( MGI:96575)
Sequence:	sense strand is shown >T36514 GGAAGACCTTCGAGGATTACCTGCACAACGTGGTTTTTGTCCCCAGGCCATCCCGAAAGCGAAGATCCCT TGAAGAGGTGGGGAATGTGACAGCCACCACACTCACACTTCCAGATTTCCCCAACGTCTCCTCTACCATT GTGCCCACAAGTCAGGAGGAGCACAGGCCATTTGAGAAAGTGGTGAACAAGGAGTCACTTGTCATCTCTG GCCTGAGACACTTCACTGGGTACCGCATTGAGCTGCAGGCATGCAATCAAGATTCCCCAGATGAGAGGTG CAGTGTGGCTGCCTACGTCAGTGCCCGGACCATGCCTGAAGCTAAGGCAGATGACATCGTTGGCCCTGTG ACTCATGAAATCTTTGAGAACAATGTTGTACACTTAATGTGGCAAGAGCCAAAGGAACCTAATGGTCTGA TTGTGCTATATGAAGTGAGCTATCGCCGATATGGTGATGAGGAGCTGCACCTCTGTGTCTCCCGGAAGCA TTTTGCCCTGGAGCGGGGCTGCAGGCTGCGAGGGCTCTCCCCAGGAAACTACAGTGTTCGAGTCCGGGCT ACCTCTCTGGCAGGAAATGGCTCCTGGACAGAACCCACCTATTTTTATGTGACTGATTATTTAGATGTCC CATCAAATATTGCCAAAATTATCATTGGACCCCTCATCTTTGTCTTCCTCTTCAGTGTTGTGATTGGAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90116. Forward Primer - name:090116_F_cDNA_Insr, sequence:GGAAGACCTTCGAGGATTACCT; Reverse Primer - name:090116_N_SP6_cDNA_Insr, sequence:CTTCCAATCACAACACTGAAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11959 same embryo
	EMAGE:11958 same embryo
	EMAGE:11957 same embryo
	EMAGE:11956 same embryo
	EurExpress:euxassay_011041 same experiment
	MGI:4825604 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source