EMAGE mouse gene expression spatial database

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EMAGE:11969

Lgi2 leucine-rich repeat LGI family, member 2 ( MGI:2180196)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011079. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011079_01	euxassay_011079_02	euxassay_011079_03	euxassay_011079_04

euxassay_011079_05	euxassay_011079_06	euxassay_011079_07	euxassay_011079_08	euxassay_011079_09

euxassay_011079_10	euxassay_011079_11	euxassay_011079_12	euxassay_011079_13	euxassay_011079_14

euxassay_011079_15	euxassay_011079_16	euxassay_011079_17	euxassay_011079_18	euxassay_011079_19

euxassay_011079_20	euxassay_011079_21	euxassay_011079_22	euxassay_011079_23	euxassay_011079_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11969_wholemount_strong.wlz

11969_wholemount_moderate.wlz

11969_wholemount_weak.wlz

11969_wholemount_possible.wlz

11969_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11969_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sublingual gland primordium

moderate

regional

moderate expression: see section 11 18 19

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 weak expression: see section 20 21

thalamus mantle layer

weak

regional

weak expression: see section 10 12 13 16 17 18

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 11 17

pons mantle layer

moderate

single cell

moderate expression: see section 12 16 17

ventral grey horn

weak

regional

weak expression: see section 11 12 13 14 15 16 17

naris

moderate

regional

moderate expression: see section 14 15 17 18

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 17 18 19 20 21 moderate expression: see section 14

esophagus

strong

regional

strong expression: see section 14 15

mandible

moderate

regional

moderate expression: see section 24 weak expression: see section 09 10 20 21 23

oral epithelium

strong

regional

strong expression: see section 12 13 18 moderate expression: see section 14 15 16 17 19 23 weak expression: see section 06 07 08 09 10 11 20 21 22

maxilla

weak

regional

weak expression: see section 09 10 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 23 24 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36586
Entity Detected:	Lgi2, leucine-rich repeat LGI family, member 2 ( MGI:2180196)
Sequence:	sense strand is shown >T36586 AAGATGACAAACTCCACCGTCTCTGATGTGCTGTGTATCGGTCCACCAGAATACCAGGAGAAGAAACTCA ACGAAGTGACCAGCTTTGACTATGAGTGTACCACCACAGGTCCCCAGACTGATGAAGCCAAGCAGAGAGG ATGGCAGTTGGAACTCTCCCTGGGATTTTGTGAACTAATATTTGTTTTTCAACACCCACTCTCAGATTTT GTCGTTCATCAGACTCTGCCGTACCAGTCGGTGTCAGTAGACACGTTCAACTCCAAGAACGATGTGTACG TGGCCATCGCTCAGCCCAGCATGGAGAACTGCATGGTGTTGGAGTGGGACCACATAGAAATGAATTTCCG GAGTTATGACAATATCACAGGCCAGTCCATCGTGGGCTGCAAGGCCATCCTCATTGACGACCAGGTCTTT GTGGTGGTGGCCCAGCTCTTCGGTGGCTCTCACATTTACAAATACGACGAGAGCTGGACCAAGTTTGTCA AGTTCCAAGACATAGAGGTGTCTCGGATTTCCAAGCCCAACGACATTGAGCTGTTCGAGATCGACGACGA GACCTTCTTCATCATCGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66865. Forward Primer - name:066865_F_cDNA_Lgi2, sequence:AAGATGACAAACTCCACCGTCT; Reverse Primer - name:066865_N_SP6_cDNA_Lgi2, sequence:GGCGATGATGAAGAAGGTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11970 same embryo
	EMAGE:11974 same embryo
	EMAGE:11971 same embryo
	EMAGE:11972 same embryo
	EMAGE:11973 same embryo
	EurExpress:euxassay_011079 same experiment
	MGI:4825912 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source