EMAGE mouse gene expression spatial database

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EMAGE:11996

Tufm Tu translation elongation factor, mitochondrial ( MGI:1923686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011105. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011105_01	euxassay_011105_02	euxassay_011105_03	euxassay_011105_04

euxassay_011105_05	euxassay_011105_06	euxassay_011105_07	euxassay_011105_08	euxassay_011105_09

euxassay_011105_10	euxassay_011105_11	euxassay_011105_12	euxassay_011105_13	euxassay_011105_14

euxassay_011105_15	euxassay_011105_16	euxassay_011105_17	euxassay_011105_18	euxassay_011105_19

euxassay_011105_20	euxassay_011105_21	euxassay_011105_22	euxassay_011105_23	euxassay_011105_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11996_wholemount_strong.wlz

11996_wholemount_moderate.wlz

11996_wholemount_weak.wlz

11996_wholemount_possible.wlz

11996_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11996_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9960
Entity Detected:	Tufm, Tu translation elongation factor, mitochondrial ( MGI:1923686)
Sequence:	sense strand is shown >T9960 GCCCGTCCTTCCCTGCACGCGCGGGCCCCGGGTGCCTAGTTCCCGGTCCCGGTCACCGAGTACTACCACA ATGGCGGCAGCCACTCTATTGCGAGCGACGCCCCGCTTCAGCGGTCTCTGCGCCAGCCCGACCCCATTTC TGCAAGGTCGGCTGCGGCCGCTGAAGGCTCCCGCATCACCCTTCTTGTGTCGCGGCCTAGCTGTGGAGGC CAAGAAGACTTATGTACGCGACAAGCCCCATGTGAATGTGGGTACCATCGGACATGTGGACCATGGCAAG ACCACCCTGACTGCAGCCATCACGAAAATTCTAGCCGAGGGAGGTGGAGCTAAGTTCAAGAAGTACGAGG AGATAGACAATGCCCCAGAGGAGCGAGCTCGGGGCATCACCATCAATGCAGCCCATGTGGAGTATAGCAC TGCTGCCCGACACTATGCCCACACAGACTGTCCTGGTCATGCAGATTATGTTAAGAATATGATCACAGGC ACTGCCCCCCTGGATGGCTGTATCCTGGTGGTGGCAGCTAATGATGGCCCCATGCCCCAGACCCGAGAGC ACTTACTGCTAGCTAAACAGATTGGGGTAGAACATGTTGTGGTGTACGTGAACAAGGCAGACGCTGTCCA GGACTCAGAGATGGTGGAGTTAGTCGAGCTGGAGATCCGGGAGCTGCTCACCGAGTTTGGCTATAAAGGA GAGGAAACTCCAGTCATCGTAGGCTCGGCTCTCTGTGCCCTTGAGCAACGTGACCCTGAGCTAGGCGTGA AGTCAGTGCAGAAGCTCCTGGATGCTGTGGACACCTACATCCCAGTGCCCACCCGGGACCTGGACAAGCC CTTCCTGCTCCCTGTAGAGTCAGTCTACTCTATTCCTGGCCGGGGCACAGTGGTGACAGGTACATTAGAG CGTGGCATTTTGAAGAAAGGAGATGAGTGTGAGTTGCTGGGACATAACAAGAACATCCGCACTGTGGTGA CAGGCATTGAGATGTTCCACAAGAGCCTGGAGAGGGCTGAGGCAGGGGATAACCTGGGTGCTCTGGTCCG AGGCTTAAAGCGCGAAGATTTGAGGCGTGGCTTGGTCATGGTCAAGCCAGGCTCCATCCAACCCCACCAG AAGGTGGAGGCCCAGGTGAGGGCTCCAGTGCTGAGTGGTTTCCCTTGCTTAGAAGCAGGACTTGTAGCTA AGCCATTCCATCCTTACTGCTTCTCTCCTCTAGGTTTATATCCTCAGCAAGGAGGAAGGTGGCCGCCACA AACCCTTTGTATCTCATTTCATGCCCGTCATGTTCTCCCTGACCTGGGACATGGCCTGTCGAGTCATCTT GCCTCCAGGGAAGGAACTTGCCATGCCTGGAGAGGACTTGAAGCTTAGTCTAATCTTGCGGCAGCCCATG ATCTTAGAGAAAGGCCAACGTTTCACCTTGAGGGATGGCAACAAGACCATTGGCACTGGCCTTGTTACTG ATGTACCAGCCATGACTGAGGAGGACAAGAACATCAAGTGGAGCTGAGTCTGGACCTCTGCTCAGGTTAT TGAGCTTCAGGCTGCCCTGGTCAGCGTTCCCACTGTGGGCATAAGGCTGCCCTGGTCAGCATTCCCACTG TGGGCATAAGGCTGCCCTGGTCAGCGTTCCCACTGTGGACACAGGCTGTATCCAGGCAGAGTACACCTCT GGACATTTCTCCTGCTCACAATGAGTGGCCTGTCAGGAGAGGGAGGCAAATAAAACTGTCCCATGGATAA CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30379757 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30379757 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11997 same embryo
	EMAGE:11995 same embryo
	EMAGE:11998 same embryo
	EMAGE:11994 same embryo
	EurExpress:euxassay_011105 same experiment
	MGI:4829021 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source