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EMAGE:12000

Kcna1 potassium voltage-gated channel, shaker-related subfamily, member 1 ( MGI:96654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12000 EMAGE:12000 EMAGE:12000 EMAGE:12000 EMAGE:12000
"Pseudo-wholemount" of euxassay_011021. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011021_01 euxassay_011021_02 euxassay_011021_03 euxassay_011021_04
EMAGE:12000 EMAGE:12000 EMAGE:12000 EMAGE:12000 EMAGE:12000
euxassay_011021_05 euxassay_011021_06 euxassay_011021_07 euxassay_011021_08 euxassay_011021_09
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euxassay_011021_10 euxassay_011021_11 euxassay_011021_12 euxassay_011021_13 euxassay_011021_14
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euxassay_011021_15 euxassay_011021_16 euxassay_011021_17 euxassay_011021_18 euxassay_011021_19
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euxassay_011021_20 euxassay_011021_21 euxassay_011021_22 euxassay_011021_23 euxassay_011021_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12000Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12000_wholemount_strong.wlz
12000_wholemount_moderate.wlz
12000_wholemount_weak.wlz
12000_wholemount_possible.wlz
12000_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12000_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
moderate moderate
single cellmoderate expression: see section 04 05 22
trigeminal v ganglion
strong strong
single cellstrong expression: see section 18 19 moderate expression: see section 04 05 06 07 08 09 20 21 22 23
dorsal root ganglion
moderate moderate
single cellmoderate expression: see section 09 10 11 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36539
Entity Detected:Kcna1, potassium voltage-gated channel, shaker-related subfamily, member 1 ( MGI:96654)
Sequence:sense strand is shown

>T36539
CCTGTCCCGCTCTGTAGATACTTTACTAAGCAGTAGTCTTTGAATGCTTTATTTTGCTGGCAATGCATTG
TTGCATTGTGAATTTGGGGAGTGGCGAACCTGAAGCTTTCAAGATCCATTAAAAAAAAAAAAAACCAAAC
ACAAAGCAAAAAATAAAAACAAAAACACAAAAATGACCCAACAACCAACTATTTTCATTTTTATTAAAAA
GAAAAGAAAGAGGATTTTCTTATAAATGCTCTCCATTAAGTAGTCTGTGTGAAATAAGACGATTTCCTTG
TCCTGAAGTATTTCCAATCTTTTGGCTTAAGGTTTTGTTTTCGTTTTTTTATTTATTTATTTTTGTTTTG
TTTTGTTTTTTCCCCTAAAAATCAGATGACCACTTAGGAATATAAAAATTCAAATCTGCATGGAACTCCA
CTCTAAACTTTTGCAAACCGCACAGCACATTTCAGATAGTGTGCTCTGTGGAACAGTTTCTAACAGCTTC
AGTGGTCAGTGTGGGGGGGGGCAATGCTTTTATTTTGATCAACTGAATTGCATACAAGGTTAACAAAATC
TGGACTAATTGAGAATGGTTCAGAAAGCACCTTGCAAATCTGATACTGGGCCCAATCTGTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94764. Forward Primer - name:094764_F_cDNA_Kcna1, sequence:CCTGTCCCGCTCTGTAGATACT; Reverse Primer - name:094764_N_SP6_cDNA_Kcna1, sequence:AACAGATTGGGCCCAGTATCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12003 same embryo
 EMAGE:12004 same embryo
 EMAGE:12001 same embryo
 EMAGE:11999 same embryo
 EMAGE:12002 same embryo
 EurExpress:euxassay_011021 same experiment
 MGI:4825695 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS