EMAGE mouse gene expression spatial database

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EMAGE:12002

Kera keratocan ( MGI:1202398)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011025. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011025_01	euxassay_011025_02	euxassay_011025_03	euxassay_011025_04

euxassay_011025_05	euxassay_011025_06	euxassay_011025_07	euxassay_011025_08	euxassay_011025_09

euxassay_011025_10	euxassay_011025_11	euxassay_011025_12	euxassay_011025_13	euxassay_011025_14

euxassay_011025_15	euxassay_011025_16	euxassay_011025_17	euxassay_011025_18	euxassay_011025_19

euxassay_011025_20	euxassay_011025_21	euxassay_011025_22	euxassay_011025_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12002_wholemount_strong.wlz

12002_wholemount_moderate.wlz

12002_wholemount_weak.wlz

12002_wholemount_possible.wlz

12002_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12002_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

strong

regional

strong expression: see section 01 02 moderate expression: see section 23

foot mesenchyme

strong

regional

strong expression: see section 01 02 22 moderate expression: see section 04 23

cranial muscle

strong

regional

strong expression: see section 05 21 22 moderate expression: see section 04 weak expression: see section 02 03 23

submandibular gland primordium

moderate

regional

moderate expression: see section 09 weak expression: see section 08 10 16 17

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 14 15

extrinsic ocular muscle

strong

regional

strong expression: see section 01 moderate expression: see section 02 weak expression: see section 03 22

nasal septum

strong

regional

strong expression: see section 13 15

lower lip

strong

regional

strong expression: see section 07 20 weak expression: see section 06

upper lip

strong

regional

strong expression: see section 06 20 weak expression: see section 07

palatal shelf

moderate

regional

moderate expression: see section 09 10 14 15 weak expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36545
Entity Detected:	Kera, keratocan ( MGI:1202398)
Sequence:	sense strand is shown >T36545 CCTCTACCTGGAAAACAACCTGATTGAATCCATACCAGAGAAGCCCTTTGAGAATGCCACCCAGCTGAGA TGGATCAATTTAAACAAGAACAAAATAACCAACTACGGGATTGAGAAGGGGGCCCTGAGCCAGCTAAAGA AACTTCTTTTCCTGTTTCTTGAAGACAATGAGCTAGAGGAGGTACCTTCTCCATTGCCAAGAAGTTTGGA ACAATTACAATTAGCTAGAAACAAAGTATCCAGAATCCCTCAGGGGACCTTCAGTAATCTGGAAAACCTG ACCCTTCTTGATCTGCAGCATAACAAACTATTAGACAACGCCTTTCAAAGAGATACTTTCAAGGGCCTTA AGAACCTCATGCAGTTAAATATGGCGAAGAATGCCCTGAGGAACATGCCCCCAAGATTACCAGCCAACAC CATGCAACTCTTTCTAGACAACAACTCCATTGAAGGAATACCTGAAAATTATTTCAATGTGATTCCTAAG GTGGCTTTCCTGAGGCTCAACCACAACAAACTATCAGATGCAGGTCTCCCGTCGAGGGGTTTTGATGTGT CATCCATTCTGGATCTTCAACTGTCTTACAACCAGCTCACAAACTTTCCCCGAATCAATGCTAACCTGCA GCACCTTCACCTTGATCACAACAAAATTAAAAATGTGAACATGTCTGTAATATGTCCCACCACACTGCGT GCAGAACAGGATGCCTTCATTCACGGACCACAACTGAGCTACCTGCGTCTGGATGGGAATGAAATCAAGC CACCGATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98004. Forward Primer - name:098004_F_cDNA_Kera, sequence:CCTCTACCTGGAAAACAACCTG; Reverse Primer - name:098004_N_SP6_cDNA_Kera, sequence:GATCGGTGGCTTGATTTCATT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12003 same embryo
	EMAGE:12000 same embryo
	EMAGE:12004 same embryo
	EMAGE:12001 same embryo
	EMAGE:11999 same embryo
	EurExpress:euxassay_011025 same experiment
	MGI:4825748 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source