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EMAGE:12009

Cdkn2aipnl CDKN2A interacting protein N-terminal like ( MGI:1261797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009
"Pseudo-wholemount" of euxassay_010988. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010988_01 euxassay_010988_02 euxassay_010988_03 euxassay_010988_04
EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009
euxassay_010988_05 euxassay_010988_06 euxassay_010988_07 euxassay_010988_08 euxassay_010988_09
EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009
euxassay_010988_10 euxassay_010988_11 euxassay_010988_12 euxassay_010988_13 euxassay_010988_14
EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009
euxassay_010988_15 euxassay_010988_16 euxassay_010988_17 euxassay_010988_18 euxassay_010988_19
EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009 EMAGE:12009
euxassay_010988_20 euxassay_010988_21 euxassay_010988_22 euxassay_010988_23 euxassay_010988_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12009Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12009_wholemount_strong.wlz
12009_wholemount_moderate.wlz
12009_wholemount_weak.wlz
12009_wholemount_possible.wlz
12009_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12009_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36230
Entity Detected:Cdkn2aipnl, CDKN2A interacting protein N-terminal like ( MGI:1261797)
Sequence:sense strand is shown

>T36230
CCTTATTTCTACCTGAACCCCCTGTACACTTGTACCTCACCACTGATGGCCACCAAAGGACCGTGCACTA
ACCAGCTGTGTCACTGGAGCACCCCCACTGCTGGGCTCAGGGACCTTTGTGTAGGAAATGGTAATCATGT
CGCAGGCTTGAAGTAGCAGAGCTAGCGCATAGGTAGGAGGCAATGTACAAAAAAGCATCCAGCCTCATTT
TACAGCTCACTGAGGCTAGAGATGTAGCTCAGTGCTAGAGTGCGTGCTTAGCGTGCTCGAGGTCCTGGGT
TGGGTCCCTAGTATTTCAAAGAGAGGAAAAAATAGATTTAAAAATCACTGGAAGCATATATGGTTTTGTT
CAACCAACCAGATGGTTTCTAGGCACTAGATGCCCATGTGCCAGCATTGTTTATTTGTTTGTTTTGTTTC
TAGAAGGCTTTCACACTTGAAGAACTAACTGCCTTGCATACCGCGTCTGGATTGCAGTGCGGTCCTGCAT
TCCACCTGACCCCGCTTCTGGTTCCCTGTATTTAGTCACCATAAGATAACTGAGCAGTGGGCAGCTGTGG
AGCAGTGTACACAGCAGGACTCATCAACG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71307. Forward Primer - name:071307_F_cDNA_D11Ertd497e, sequence:CCTTATTTCTACCTGAACCCCC; Reverse Primer - name:071307_N_SP6_cDNA_D11Ertd497e, sequence:CGTTGATGAGTCCTGCTGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12010 same embryo
 EMAGE:12007 same embryo
 EMAGE:12006 same embryo
 EMAGE:12005 same embryo
 EMAGE:12008 same embryo
 EurExpress:euxassay_010988 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS