EMAGE mouse gene expression spatial database

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EMAGE:12028

Grid1 glutamate receptor, ionotropic, delta 1 ( MGI:95812)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008381. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008381_01	euxassay_008381_02	euxassay_008381_03	euxassay_008381_04

euxassay_008381_05	euxassay_008381_06	euxassay_008381_07	euxassay_008381_08	euxassay_008381_09

euxassay_008381_10	euxassay_008381_11	euxassay_008381_12	euxassay_008381_13	euxassay_008381_14

euxassay_008381_15	euxassay_008381_16	euxassay_008381_17	euxassay_008381_18	euxassay_008381_19

euxassay_008381_20	euxassay_008381_21	euxassay_008381_22	euxassay_008381_23	euxassay_008381_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12028_wholemount_strong.wlz

12028_wholemount_moderate.wlz

12028_wholemount_weak.wlz

12028_wholemount_possible.wlz

12028_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12028_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08

corpus striatum

moderate

single cell

moderate expression: see section 03 04 05 06 21 22 23 weak expression: see section 07 08 20

telencephalon mantle layer

moderate

single cell

moderate expression: see section 01 02 03 04 05 09 10 11 12 13 15 16 17 18 21 22 23 weak expression: see section 07 08 19 20

olfactory cortex marginal layer

moderate

single cell

moderate expression: see section 11 12 13 15 16 17 18

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

pons mantle layer

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20

midbrain mantle layer

moderate

single cell

moderate expression: see section 09 10 11 12 14 15 16 17 18 weak expression: see section 08

ventral grey horn

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35229
Entity Detected:	Grid1, glutamate receptor, ionotropic, delta 1 ( MGI:95812)
Sequence:	sense strand is shown >T35229 CTGGCTAAGGCTCTAGGATTCAAATATGAGATATACCAGGCCCCTGATGGCAGGTATGGTCACCAGCTCC ATAACACCTCCTGGAATGGGATGATCGGGGAGCTCATTAGCAAGAGAGCAGACTTGGCCATCTCTGCTAT TACCATCACCCCAGAGAGAGAGAGCGTTGTGGACTTCAGCAAGCGCTACATGGACTACTCAGTGGGGATC CTCATCAAGAAGCCCGAGGAGAAGATCAGCATCTTTTCCCTTTTCGCCCCCTTTGACTTTGCGGTGTGGG CCTGCATTGCCGCAGCCATTCCTGTGGTGGGCGTGCTCATATTCGTGTTGAATCGGATACAGGCTGTAAG ATCTCAGAGTGCCACCCAGCCACGACCCTCGGCTTCTGCCACCCTGCACAGTGCCATTTGGATTGTCTAT GGAGCCTTTGTCCAGCAAGGTGGTGAGTCTTCGGTGAACTCTGTGGCCATGCGCATCGTGATGGGCAGCT GGTGGCTCTTCACCCTCATTGTGTGTTCCTCCTACACAGCCAACCTGGCCGCTTTCCTCACAGTGTCTAG GATGGACAACCCCATAAGGACATTTCAGGACCTGTCCAAGCAACTGGAGATGTCTTATGGCACTGTCCGG GACTCTGCTGTCTATGAGTACTTCAGAGCCAAGGGGACCAATCCCCTGGAGCAGGATAGCACTTTTGCTG AGCTCTGGCGGACCATAAGCAAGAATGGAGGGGCTGACAACTGTGTGTCCAATCCTTCAGAAGGTATCAG GAAGGCAAAGAAGGGGAACTACGCCTTTCTATGGGATGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97789. Forward Primer - name:097789_F_cDNA_Grid1, sequence:CTGGCTAAGGCTCTAGGATTCA; Reverse Primer - name:097789_N_SP6_cDNA_Grid1, sequence:CACATCCCATAGAAAGGCGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12029 same embryo
	EMAGE:12030 same embryo
	EMAGE:12032 same embryo
	EMAGE:12031 same embryo
	EurExpress:euxassay_008381 same experiment
	MGI:4825229 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source