EMAGE mouse gene expression spatial database

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EMAGE:12032

Gpr83 G protein-coupled receptor 83 ( MGI:95712)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008380. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008380_01	euxassay_008380_02	euxassay_008380_03	euxassay_008380_04

euxassay_008380_05	euxassay_008380_06	euxassay_008380_07	euxassay_008380_08	euxassay_008380_09

euxassay_008380_10	euxassay_008380_11	euxassay_008380_12	euxassay_008380_13	euxassay_008380_14

euxassay_008380_15	euxassay_008380_16	euxassay_008380_17	euxassay_008380_18	euxassay_008380_19

euxassay_008380_20	euxassay_008380_21	euxassay_008380_22	euxassay_008380_23	euxassay_008380_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12032_wholemount_strong.wlz

12032_wholemount_moderate.wlz

12032_wholemount_weak.wlz

12032_wholemount_possible.wlz

12032_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12032_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoralis major

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 17 18 19 20 21 22

pectoralis minor

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 17 18 19 20 21 22

thymus primordium

weak

single cell

weak expression: see section 12 13 14 15 16

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 13 14 weak expression: see section 08 09 10 11 17 18 19

midbrain mantle layer

moderate

single cell

moderate expression: see section 06 07 18 19 weak expression: see section 08

mandible

moderate

single cell

moderate expression: see section 02 03 07 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35222
Entity Detected:	Gpr83, G protein-coupled receptor 83 ( MGI:95712)
Sequence:	sense strand is shown >T35222 ATGTCAGTCGCTTTGCTCAGTACTGTTCTCTACATGTCTCAGCACTGACTCTGACAGCTATCGCAGTGGA CCGCCACCAGGTCATCATGCATCCACTGAAGCCTCGGATCTCCATCACCAAGGGTGTCATATATATTGCT GTCATCTGGGTCATGGCTACCTTCTTCTCTCTGCCACATGCCATCTGCCAGAAACTGTTTACCTTCAAGT ACAGTGAGGACATTGTGCGCTCCCTCTGCCTGCCGGACTTCCCGGAGCCAGCTGACCTCTTCTGGAAGTA TCTGGACCTGGCCACCTTCATCCTGCTCTACCTACTTCCACTCTTCATTATCTCAGTGGCCTATGCTCGT GTGGCCAAGAAGCTGTGGCTCTGTAACACCATTGGCGACGTGACCACAGAGCAGTACCTCGCCCTGCGAC GCAAGAAGAAGACCACCGTGAAGATGCTGGTGCTTGTGGTAGTCCTCTTTGCCCTCTGCTGGTTCCCTCT CAACTGCTATGTCCTCCTCTTGTCCAGCAAGGCCATCCACACCAACAATGCCCTCTACTTTGCCTTCCAC TGGTTTGCCATGAGCAGTACTTGTTATAACCCCTTCATCTACTGCTGGCTCAATGAGAACTTTAGGGTTG AGCTTAAGGCATTGCTGAGCATGTGCCAAAGGCCACCCAAGCCGCAGGAAGACAGGCTACCCTCCCCAGT TCCTTCCTTCAGGGTGGCATGGACAGAGAAGAGCCATGGTCGGAGGGCTCCACTACCTAATCACCACTTG CCCTCTTCCCAGATCCAGTCTGGGAAGACAGATCTGTCATCTGTGGAACCCGTTGTGGCCATGAGTTAGG GAAAGCTGGAAGTTGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99079. Forward Primer - name:099079_F_cDNA_Gpr83, sequence:ATGTCAGTCGCTTTGCTCAGTA; Reverse Primer - name:099079_N_SP6_cDNA_Gpr83, sequence:CACCAACTTCCAGCTTTCCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12029 same embryo
	EMAGE:12030 same embryo
	EMAGE:12028 same embryo
	EMAGE:12031 same embryo
	EurExpress:euxassay_008380 same experiment
	MGI:4825194 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source