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EMAGE:12058

Muc15 mucin 15 ( MGI:2442110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058
"Pseudo-wholemount" of euxassay_008588. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008588_01 euxassay_008588_02 euxassay_008588_03 euxassay_008588_04
EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058
euxassay_008588_05 euxassay_008588_06 euxassay_008588_07 euxassay_008588_08 euxassay_008588_09
EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058
euxassay_008588_10 euxassay_008588_11 euxassay_008588_12 euxassay_008588_13 euxassay_008588_14
EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058
euxassay_008588_15 euxassay_008588_16 euxassay_008588_17 euxassay_008588_18 euxassay_008588_19
EMAGE:12058 EMAGE:12058 EMAGE:12058 EMAGE:12058
euxassay_008588_20 euxassay_008588_21 euxassay_008588_22 euxassay_008588_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12058Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12058_wholemount_strong.wlz
12058_wholemount_moderate.wlz
12058_wholemount_weak.wlz
12058_wholemount_possible.wlz
12058_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12058_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36249
Entity Detected:Muc15, mucin 15 ( MGI:2442110)
Sequence:sense strand is shown

>T36249
ATCCTTTACAGGTCTCCGAACATTCCAATTCTACAAATTCCCCATCACCAGAAAACTTTACTTGGTCTTT
GGACAATGACACAATGAACAGTCCTGAAGATATTTCCACTACAGTAAGACCCTTCCCTCCACCTCCAAAG
ACCACACCTGTGACCCCTTTCACAGCTGAACCTACTGAATGGCTCCCCACAAACAATGACAACTTTGCTG
GTTTTACCCCGTATCAAGAAAAAACAACTTTACAACCTACACTAAAATTCACCAATAATTCAAAACTTTT
CCCAAATACATCAGACACCCCAAAAGAGAATAAGAACACAGGAATAGTATTTGGAGCCATTTTAGGAGCC
ATCCTGGGTGCTTCACTGCTTAGCCTTGTTGGCTACTTATTGTGTGGACAACGGAAAACAGATTCATTTT
CCCATCGGCGCCTTTATGATGACAGAAACGAACCAGTTCTGCGATTAGACAATGCACCAGAACCTTATGA
TGTCAATTTTGGAAATTCTAGTTACTACAACCCAGCTGTGAGTGATTCATCCATGCCAGAAGGGGGAGAA
AGCCTGCAAGATGGCATTCCTATGGATGCTATACCTCCACTTCGCCCCTCAATATAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67822. Forward Primer - name:067822_F_cDNA_D730046L02Rik, sequence:ATCCTTTACAGGTCTCCGAACA; Reverse Primer - name:067822_N_SP6_cDNA_D730046L02Rik, sequence:TTATATTGAGGGGCGAAGTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12057 same embryo
 EMAGE:12059 same embryo
 EMAGE:12060 same embryo
 EMAGE:12055 same embryo
 EMAGE:12056 same embryo
 EurExpress:euxassay_008588 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS