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EMAGE:12063

Cwf19l2 CWF19-like 2, cell cycle control (S. pombe) ( MGI:1918023)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063
"Pseudo-wholemount" of euxassay_009562. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009562_01 euxassay_009562_02 euxassay_009562_03 euxassay_009562_04
EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063
euxassay_009562_05 euxassay_009562_06 euxassay_009562_07 euxassay_009562_08 euxassay_009562_09
EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063
euxassay_009562_10 euxassay_009562_11 euxassay_009562_12 euxassay_009562_13 euxassay_009562_14
EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063 EMAGE:12063
euxassay_009562_15 euxassay_009562_16 euxassay_009562_17 euxassay_009562_18 euxassay_009562_19
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euxassay_009562_20 euxassay_009562_21 euxassay_009562_22 euxassay_009562_23 euxassay_009562_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12063Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12063_wholemount_strong.wlz
12063_wholemount_moderate.wlz
12063_wholemount_weak.wlz
12063_wholemount_possible.wlz
12063_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12063_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36217
Entity Detected:Cwf19l2, CWF19-like 2, cell cycle control (S. pombe) ( MGI:1918023)
Sequence:sense strand is shown

>T36217
GGACACTGCTTCATAGTCCCTCTGCAACATCATCAAGCAGCCACTGTATTAGATGAAGATGTCTGGGAGG
AGATCCAGATGTTTCGAAAATCATTGGTAAAAATGTTTGAAGATAAAGAATTGGATTGTATCTTTTTGGA
GACTAATATGGGTTTGAAGAAACACTATCATATGGTTTATGAGTGTATCCCTCTCCCAAAGGAAGTGGGT
GATATGGCTCCCATCTATTTTAAGAAAGCCATAATGGAATCTGATGAAGAATGGTCCATGAATAAGAAGT
TGATTGATTTGTCTTCAAAAGATATTAGAAAGTCTGTGCCCAGAGGTTTGCCTTACTTTGCTGTCGACTT
TGGTCTTCAGGGAGGTTTTGCCCACATCATTGAAGATCAGTACAGATTCCCTCATTACTTTGGAAAGGAA
ATCATTGGTGGTATGCTGGATCTAGAACCTAGACTATGGAGGAAGGGGATCCGAGAAAGCTTTGAGGACC
AGAGGAAGAAATCATTGCAGTTTGCTCAGTGGTGGAAACCATATGATATCACCAAAAGTAAAAGCTCTTA
AGTGTAGGTTATGTTTCATTTTCTATGCTGAACCCATTCAGTTTATGTCTATTATATTTACAAACAACTG
TGCTTTGGGCTCTAGGGAAAGCAGCAGCAGCAGCAGCTGGCCACTCTAGTCACTGACAGCCATATTAGGT
CAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65400. Forward Primer - name:065400_F_cDNA_Cwf19l2, sequence:GGACACTGCTTCATAGTCCCTC; Reverse Primer - name:065400_N_SP6_cDNA_Cwf19l2, sequence:TTGACCTAATATGGCTGTCAGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12064 same embryo
 EMAGE:6093 same embryo
 EMAGE:12062 same embryo
 EMAGE:12066 same embryo
 EMAGE:12061 same embryo
 EurExpress:euxassay_009562 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS