EMAGE mouse gene expression spatial database

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EMAGE:12083

Golim4 golgi integral membrane protein 4 ( MGI:1920374)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008348. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008348_01	euxassay_008348_02	euxassay_008348_03	euxassay_008348_04

euxassay_008348_05	euxassay_008348_06	euxassay_008348_07	euxassay_008348_08	euxassay_008348_09

euxassay_008348_10	euxassay_008348_11	euxassay_008348_12	euxassay_008348_13	euxassay_008348_14

euxassay_008348_15	euxassay_008348_16	euxassay_008348_17	euxassay_008348_18	euxassay_008348_19

euxassay_008348_20	euxassay_008348_21	euxassay_008348_22	euxassay_008348_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12083_wholemount_strong.wlz

12083_wholemount_moderate.wlz

12083_wholemount_weak.wlz

12083_wholemount_possible.wlz

12083_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12083_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9261
Entity Detected:	Golim4, golgi integral membrane protein 4 ( MGI:1920374)
Sequence:	sense strand is shown >T9261 CTTAACCTGACCAGACCAACCAGTCCCCATCTCTTCATCAATACCGAGGGACCGTTCAAGGGGACGCCGC CTGGAGCCAGGTCCCTTTGTTGGGCGCGCGTCCCCTACCAGTGGTAGCGACAAAGTCCAGCAGCGGGAGC CGGCGCGAGGGGCGGGGACTGACGGTGGGGACAGCGAGGCCAAAAAGTGGGGAGTAGGGAAAACCAAGCC GGCTGCGCGCGGGGACCAGTAGCAAGGGCAGCGGGCTCTGAAGTATGCAAAGAGACTCCACGCGGGCCCA GCCACTCGCGGAAATCCGACATCGCAGAAGTTTGGTGGCTGCTGGCCGGGTGGCGTCGATGACCTGGCGC CCAGCCGGCCGCGGGGGCTGAGCGGCCGCCTCTTCCTCTCTGGTCCGGCTTTTGTGTCTCGCGTCTTCTG CTCATGCCGTGTCCAGCTACTTCTCGCGGTGGCCGTTGCTGAGCTGTCCGACGCAGCCGAGGACCGAGGA CACGACGGCTCCCGCCGCCGCAGCGCGGGGCACAGGAGGGACTATGGGAAACGGGATGTGCTCCCGAAAG CAGAAGCGGATCTTCCAGACGCTGCTGCTTCTCACGGTGGTCTTCGGCTTCCTCTATGGCGCGATGCTCT ACTTGGAACTGCAGACGCAGCTGCGCAAAGCTGAGGCAGTGGCGCTCAAGTACCAGCAGCACCAGGACTC TCTCTCTGCCCAGTTGCAAGTTGTATATGAACACAGATCAAGATTAGAGAAATCTTTGCAAAAAGAAAGG CTTGAACACAAGAAAGCAAAGGAAGATTTCCTTGTTTATAAGTTAGAAGCACAAGAAACACTTAACAAAG GAAGGCAAGATTCCAATAGCAGATATAGTGCATTGAATGTCCAACATCAGATGCTGAAAAGTCAGCATGA GGAGCTGAGGAAGCAGCACAGTGACCTGGAAGAGGAACACCGGAAGCAAGGGGAAGACTTCAGTAGAACG TTCAATGACCACAAGCAGAGATACCTGCAGCTTCAGCAAGAAAAGGAACAAGAACTTTCTAAGCTAAAAG AAACTGTATATAATTTGAGAGAAGAGAATAGACAACTAAGAAAAGCACACCAAGACATTCACACACAGCT TCAAGACGTCAAGACTCAAGTTGCAGAATATAAACAACTGAAAGATACACTGAACAGGATTCCAAGCTTT CGAAATCCTGACCCAGTCGAGCAGCAAAATGTCACCTTTCCCCATGGGACTCACCCTCCTCAAGGTTACA ACGGAAGAGAGAAGCTGACAGGAGAGCTCCAAGAGGTGCAGCCGAATCACGAAGCAGGGCCTAGAAGAAT GGAAGAGAAACCTCTGTCTTCCATGCAGAAGGATGCAGGATTCCAGGCTCTAGAAGAGCAGAATCAAGTG GAACCCAGAGAGCCAGAGGAGCGTCAAGTGGAAGAGGAACACAGGAAGGCACTGGAGGAGGAGGAGATGG AGCAGGTGGGGCAGGCGGAGCATCTGGAGGAAGAACACGACCCATCACCAGAGGAGCAGGATCGAGACTG GCGGGATCAGCAGGGACAGAATGCAGCCCACCTTCTGGATGGCCACCCTCAAGCTGAGGTGGAGCATTCG ACCAAGGCAGCTACAAACTTCCAATCCCCATATGAGGAACAGTTGGAACAGCAGAGGCTGGCAGCGAGGA GAGATGAGGAAGCCCAGCGGCTGAGAGAACATCAGGAAGCCTTGCACCAACAGAGGCTCCATGGGCAGTT GCTAAGACAACAGCAGCAGCAACAGTTCCTGGCCAGAGAGATGGCTCAGCAGAAGCAGGTAGCCCATGAA GATGGCCAGCAGCAGCACCAGGAGCAGCTAAGGCAGCAAGCTCATTACAATGCTGTGGAGAATGACATCG CACAGGGAGTCGAGGACCAGGGAATCCCCGAAGAAGAGGGAGGTGCCTATGACAGAGATAACCAGCGCCA AGATGAAGCTGAAGGAGACCCAGGCAACAGACAGGAACTTCGTGAACCAGGACACCAAGAAGGCGACCCT GAAGTTGAAGCAGATAGGGCAGCTGTGGAGGATATAAACCCAGCAGATGACCCCAACAATCAAGGCGAAG ATGAATTTGAGGAAGCCGAGCAAGTGAGAGAAGAAAATTTGCCAGAGGAAAGTGAAGAGCAAAAACAAAG CGAAGCAAAGCAAGGGAATGTAGAAATGGATGAGCACTTGGTGATGGCAGGGAATCCAGACCAGCAGGAG GATAATGTTGATGAGCAGTATCAGGACGAAGGAGAGGAAGAGGTTCAGGAAGATCTGACTGAAGAGAAAA AACGGGAAATGGAGCATAATGTTGAAGAGACATATGGTGAACATCCTGATGATAAGAATAATGATGGTGA AGAACAAGGAGTTCACAACAGGGCGCATCCTAAAGGAAGACAGGAGCATTATGAGGAGGAGGAAGATGAA GAAGACGGGGCTGCTGTTGCTGAGAAATCACATCGCAGAGCTGAAATGTAACTGGACTCAGTTTCTAGAC TGTGCTCAGCCAACGATTTTTTTCTCAAGCTGCTCTAAAACAAATCCCGCCTACTGAATTCTAGAGTATT TAATTACATACAGAAGTAAGGGTTTAGACTTTTTTAACTTTTTTATTAGAAATGCTATACATTTATATGA ATATATTTTGATACCTTAGGTGAGAACTTCTTTTATAGTCGAGTTATACATGGACAAGAAGAAAAGCAGT AAGACTTGCCTTCAGGTCTGAATCTCATTTCCCAGCGATGGTGGGAAGATGTGCTTGGCTTTGGTGCATG TCTCAGCTCAGTACCATTTTGTTTTCTGGCTTCCGGGTAGTCTACTATTTATCTCAGATAAAATGCAAGA TATAGAAAAAAAAAGGTGTATGGAAAAACAATATGCAAACATCAGGGGAATGGCTGGTGTCCTCGTGGCT GTTCTTGCAGGTTCTTGTATACAGCTCTGAAAGAATGTCACTACTGCAGAGTGTTGGCAAATGAGAGCCA TCTGTACAGTTGAAAATCAATTGGAAAGCCCGTGTGTCTGTGTCGGTGTGTGTGCGTGCGTGTCAGGACA AAGTTTTTTTTCAAACTTTCAGGTCACTTTGAATAATACGTAGCAATTCTTAAAATCACAGCTATCTTGC TTGGGAGGAATCACGTATGAAAGTTGGATAATATTAATACCCCTATGAAAGTCTCAAGGACTCTGTCTTG TCAGAGAGGCCAAAGGAGCTGACACACTTAGAAATAGATGCAGTATGTTCAGTGATGCTTCCTGGGTGAA CTCCCCGTCTCCCCATCGGTTATCTGCTTGCTCGTCAGTCTCCACTTGCTCCAGCATATTTTCCTCTCTT AAGCTCTGTGCCTTGATGTGGAACCATTTGGTTCACAGTTATGGATAGGAAAGCATGCGAAGCTACAGTT TAATAGGATTATTTTTCAGACTATATGATAAAATAACTATAGCACTAGGATAGGCACCCCTCCCTTAGCG GCTTTACTATTTACCCAGAATTGACCTGCCTTTGCCTATGTATGAGGCATTCTAATGGCTATCAAGAATA ATGCATCCTTTAAAAGAAAACTCAAACTGAAAGGTTTTCCCTGAAATGAGCACATATTTTAGTTTACATC ATCAATATTGTTAAATACATTGAACTGGGCCACCGTTAATCTGTGTGTGGTACTTTCAAAATAATAAAGC AAATTTCATTTTCTGTTATTATCATATGAGTGTAATTTTAGTTTCTAATAACCACAATCATGTATGGTTT TGATTTTCCCAGCTGAGCAATTGTGTATGTATGCATAGGAAATGTTCCGCTCAAATTTAGTTTGGATTTC CAGTAACAATAAATCAAATCTCATCTTTATGTATAAAAGCCCCATATGTAATGTAAAATGTATTATATTG TACATAAATACTCAGAATACAATTATTTTGGTAAATTAGTTTGTATTTTTAATGTCCCTTTGCAGTATTT AATTATTTGAAACACATTGAAACTTGGGTAATAATCAATAAACTTATAAAAAGTTAAGGATAAGTCTTTT AAAGCATATAATTTCTTAGTGCTTTGTTATCTATGGTTTTTCTTTATAGGACAATATAAATTAATGAATT ATTAAATGCCTTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837211 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837211 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12086 same embryo
	EMAGE:12085 same embryo
	EMAGE:12082 same embryo
	EMAGE:12084 same embryo
	EMAGE:12087 same embryo
	EurExpress:euxassay_008348 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source