EMAGE mouse gene expression spatial database

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EMAGE:12101

Stox2 storkhead box 2 ( MGI:1918319)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008526. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008526_01	euxassay_008526_02	euxassay_008526_03	euxassay_008526_04

euxassay_008526_05	euxassay_008526_06	euxassay_008526_07	euxassay_008526_08	euxassay_008526_09

euxassay_008526_10	euxassay_008526_11	euxassay_008526_12	euxassay_008526_13	euxassay_008526_14

euxassay_008526_15	euxassay_008526_16	euxassay_008526_17	euxassay_008526_18	euxassay_008526_19

euxassay_008526_20	euxassay_008526_21	euxassay_008526_22	euxassay_008526_23	euxassay_008526_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12101_wholemount_strong.wlz

12101_wholemount_moderate.wlz

12101_wholemount_weak.wlz

12101_wholemount_possible.wlz

12101_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12101_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 15 16 17 weak expression: see section 10

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 14 15 16 17 18 weak expression: see section 03 04 05 06 07 08 09 10 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 14 15 17 18 weak expression: see section 11 12 13 16 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35693
Entity Detected:	Stox2, storkhead box 2 ( MGI:1918319)
Sequence:	sense strand is shown >T35693 GAGTATGAATTTTGCGACCCTCTAACCAGGGCCCCCAGAGAGGGCTGCTTCATCATTGAACACAAAGGGG ACAACTTCATCATGCATAGCAACACGAACGTGATCGAGTCCCATTTCCCCATGACGCCAGAATGGGATGT GTCTGGCGAACTGGCCAAAAGGAGAACGGAGATGCCCTTTCCCGAACCCTCCAGGGGAAGCTCCCACTCC AAAGTGCACCGAAGCCACAGCCATACCCAGGACCGGAGATCCAGGAATGAGAGGTCCAACAAGGCCAAGG AGAGATCCAGGTCTATGGATAACTCCAAAGGTCCCCTGGGCGCTTCTTCACTCGGGACTCCGGAAGACCT GGCTGAAGGCTGCAGCCAAGATGACCAAACACCGAGCCAATCATACATTGATGACAGTACTTTAAGGCCT GCGCAAACGATTGGTCATCAAAGGGCTCATATTCCTTCTGCCAGCTACAAAGAGGTATGCATTCCAGAAA TAGTTGGTGGCAGCAAGGAACCTTCTAGTGCTTGTAGCCTTCTGGAGCCAGGCAAAACCCCTGAGAGTAT GCCGTCCTATGGGGAACTCAGCCCTTGCCCGGCTAAAACAGCTGTGGATGACTATTTTCAGTGCAACACC TCGAGTGAGACCGTACTCACAGCACCGTCCCCTTTAGGGAAGAATA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68202. Forward Primer - name:068202_F_cDNA_4933409N07Rik, sequence:GAGTATGAATTTTGCGACCCTC; Reverse Primer - name:068202_N_SP6_cDNA_4933409N07Rik, sequence:TATTCTTCCCTAAAGGGGACGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12105 same embryo
	EMAGE:12102 same embryo
	EMAGE:12100 same embryo
	EMAGE:12104 same embryo
	EMAGE:12103 same embryo
	EurExpress:euxassay_008526 same experiment
	MGI:4828512 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source