EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12121

Klhl23 kelch-like 23 (Drosophila) ( MGI:2683536)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008410. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008410_01	euxassay_008410_02	euxassay_008410_03	euxassay_008410_04

euxassay_008410_05	euxassay_008410_06	euxassay_008410_07	euxassay_008410_08	euxassay_008410_09

euxassay_008410_10	euxassay_008410_11	euxassay_008410_12	euxassay_008410_13	euxassay_008410_14

euxassay_008410_15	euxassay_008410_16	euxassay_008410_17	euxassay_008410_18	euxassay_008410_19

euxassay_008410_20	euxassay_008410_21	euxassay_008410_22	euxassay_008410_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12121_wholemount_strong.wlz

12121_wholemount_moderate.wlz

12121_wholemount_weak.wlz

12121_wholemount_possible.wlz

12121_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12121_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9180
Entity Detected:	Klhl23, kelch-like 23 (Drosophila) ( MGI:2683536)
Sequence:	sense strand is shown >T9180 AGTAACTCGAGAATCTTAGGTGCCTGGATTCAGCCTTGACCTAGGCTACCCCCACTTTCCCTGGAAGCTT CCAGGTTAACTCCTAGTGTGCTTAAGCAGCCCCAAAGCTCCTTCCTGCGCGCTCTGCTGGAGGCAGCATG GAGTTTATGGGAAAGCAGCTACTTGTGGCATTTGAAGGCAGCCCTAATAGAAGACCGTAGTTTCGCCTTC TTCTCTCGGCACTTTCTCTCTCCCCGCACGCCAAGGGCCAGGGCCGGCAAGAGGACAGAGAAGCATGGCT CTAAAAGGACAAGAAGATTACATTTTTCATTTCAAGGATTCGTCGCATCCAGTGGATTTTCTGGATGCAT TCAGAACGTTTTACATGGATGGACTATTTACTGATATTACCCTTCAGTGTCCTTCAGGCATAATCTTCCA TTGTCACCGAGCTGTTTTAGCTGCCTGCAGCAATTATTTTAAGGCAATGTTCACAGCTGACATGAAAGAA AAATTTAAAAGTAAAATAAAACTGTCGGGCATCCACCATGATATTTTGGAAGGCCTTGTAAATTATGCAT ATACTTCTCAAATTGAAATTACTAAGAGAAATGTACAGAGCCTTCTTGAAGCAGCGGATCTGCTGCAGTT TCTTTCTGTAAAGAAGGCGTGTGAGCAGTTTCTGGTCAGGCACCTGGACATTGATAACTGTATCGGGATG CACTCTTTTGCAGAATTCCATGTGTGCTCAGAACTAGAGAAGGAATCCCGCAGGATCCTCTGCTCCAGGT TTAAGGAAGTTTGGCAGCAAGAAGAATTTCTGGAAATCAGCCTTGAAAAGTTTCTCTTTATCTTGTCCAG AAAGAATCTGAGTGTCTGGAAGGAAGAGGCTATCTTAGAGCCGGTCATTAAGTGGACTGCTCACGATGTA GAGAATCGAATCGAATGCATCTACAACCTGCTAAGCTATATCAACATAGACATAGACCCAGTGTACTTAA AGACGGCTCTAGGCCTTCAAAGAAGCTGCCTGCTAACTGAAAATAAGATACGTTCCCTAATATACAATGC CTTGAATCCCATGCATAAAGAGATTTCCCAGAGGTCCACAGCCACCATGTATATCATTGGAGGCTACTAC TGGCATCCTTTATCAGAGGTCCACATATGGGACCCTTTGACAAATGTTTGGATTCAGGGAGCAGAAATAC CAGATTATACCAGGGAGAGTTACGGTGTTACCTGCTTAGGACCTAACATTTATGTGACTGGCGGGTACAG GACAGATAATATAGATGCTCTTGACACAGTGTGGATCTATAACAGTGAGGGAGATGAGTGGACAGAAGGC CTGCCCATGCTCAATGCCAGGTATTACCACTGTGCAGTCACCTTGGGGGGCTGTGTCTACGCACTAGGTG GTTACAGGAAAGGGGCTCCTGCCGAAGAGGCCGAGTTCTATGATCCTCTGAAAGAGAAGTGGCTTCCTAT CGCAAACATGATTAAAGGTGTGGGAAATGCTACTGCCTGTGTCCTACATGAAGTCATCTATGTCATTGGT GGCCACTGTGGCTACAGAGGAAGCTGCACCTATGACAAGGTGCAGAGCTACAATTCAGACATCAACGAAT GGAGCCTTATTACTGCGAGCCCACACCCAGAATATGGACTGTGCTCAGTTCCATTTGAAAACAAGCTCTA CCTAGTCGGCGGACAGACCACGATCACAGAGTGCTATGACCCGGAACAGAACGAGTGGAGAGAGACGGCG CCTATGATGGAAAGGAGGATGGAGTGTGGGGCTGTCATCATGAATGGATGCATCTACGTAACCGGGGGCT ACTCCTACTCAAAGGGGACGTATCTGCAGAGTATCGAGAAATACGATCCAGATCTTAATAAGTGGGAAAT AGTGGGCAATCTCCCGAGTGCCATGCGGTCTCATGGATGCGTTTGTGTGTATAATGTCTGAGTCCACAGG ACTAACCAGGTCCTCACTAGGAATGTAGTCAAGAGTGCAGAGTCTATGGTGCCTTGGTATTGTGTTTTGG CACATGCTAGGGCACTGGAAATTTTTAATTCTAACCCTGCACCATTCTCAGACCCAGTAATTAATGGGAA TAACAAAAGAAAAATGTGATGTGTGTTTAACAGTTCAGTTAGGAGAGTTAACCCCTCCTAGAATCTCTGC TTTTTAAAGGTAGAATGGTGTATTTGACGCTTGGTAAAGGTGAAAAGTCTTTGTAGTGAATCTTTTCTTC CTGGTAGCATCAGCACTGGGTATAGGTCAAAATCATTCATTCTGAGTTATAAAATGTCTGTTAAAAGTCT GATGGGCATGGTACACATGCCTTTAATCCCAGAACTTGGGAGGCAGAGGCAGGCAGATCTCTGAATTGAA GGACATCAAGACCAGCCTGGTCTACATAGCAACTTCCAGGCCAGCCAAAGCTACATTGAGAACCTGCCTT TAAAAGTCTATGATGTGCTGTGTATGTTACAGGTCTCTAGCTGGCAGGGATCTGAAATGTAAAGAGGGAA CCTTCTCTACCTCTACAAAACAGCACTTTAAAAGTTTCCCTTTAGAATTAGTTAGAACGTGGATTTACTC ACAGTCTCTTTCCTTTGTGGTGTATATGATTTATTGCTAATATTCATTATTTACTATCAGAGCTACACTA AACACTGTACAAGTGATTGTAACTGTTCAAAGAGTTTACAACTCAATTTAAAAGAATAGCAACTCTAAAA AGGACTGAGTCAACAGTCAAATATTTGCTGATGCTAAGGGCTGTTTTCATTTTTAGCTTGGGTATAAGTT GCTAGTTTAGGTTCTGGCTTAAGCATGTTGGCAAATCCTCTTTCTAGTTAGATGTCTGCGTAGACAATGG AATGCCAGGGCCAGAAAGCATGTAGAACAGCTGCTAGGCAGTGGAACACACATCAAAGAGAAAAATTTGC TTTTATTTGCCAGCTCTGCCACTAACCAGATTTTGTGACTTCAGTCTCTGAGTACATCTGCTTCTTCATC CATGTACACCGGAGACATGGATTTTTCTGTATCCAGAGCCGCTGGACCCTTTAAGCCAGATGAGGTCTGG GTGGCCTATGAGCCAGATATACCCTTTTCCTCTGTGTGCTTGCAGGGTCAGTCCCCAAATGTGCCATGAC CTGAGAAAAGGGGGAAGCGCTGCTCAAGTCACTCATCTAAACGTGAGACTGTCTGCTCCACGCCAGGTTG GTTTTATTGTTTTGTTTTTAAAGAGAGAATACTTAATTTTTACTTTAAATAAAAACTACTTCTGACGGAA AAAAACTTTTAAGAAGACTATTTAATAATCCCTTCGGGTGGCTTTTTAAATAAATACAACCCTTTGGAGT TGCTATACGGTACTTTTTTATAAAGAGTCATAAAAGTGACTGTCCTGGGCTAGTGAGATGACTAGCAGGT GAGATTGCTTACCTCGCAGGCCCAAGAGCTGCATTTAACCCTGAGAGGAGAACATGGACTCCTGGGGGCT GTCCTCTGCCTTTCACATGTGTGCTGTGCTACATGTACACACACACACACACACACACACACACACGATG GAAGGAAATTCGATTTTTAAAGATGACTGTGTCCTTTGCCCTGTGAGCTGTCATTATTTTTAAAGAATGA TGAAGGATGTAAATGCATGGGAATGTTTATGTCTGCATTGTAAACAATAGTCACAGTACAGAGAAGATCT TCATGTTAAAGAAAGTGTGCTAATGGAATAGTGAGGTGTGCCGCCATGGCAGTAACTGTTGGGAAGACTG TAGACGTACGATGTGTCTATGCTAAGCAAGCACGGTGCAAGCTAGTACTCACACTGTCACACCTTTACGC ATGCGAAAGGTAATGTGCTAAATAACACAAGCTGTTAGTGTAAGGGGATGTGGAATGGTCTTAATGAATT TTTTTCTTTGTATTTTCAATAAAAATAAGTAAGTCACCGTACATGTGGTTAAGATGTCCTACATGTGAGC CAACTGGGTTGAAGCAGTCTAACAGTGAAATAAATTATATTGCACTAAAATCTGTAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827841 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827841 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12122 same embryo
	EurExpress:euxassay_008410 same experiment
	MGI:4825797 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source