EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12134

Efhb EF hand domain family, member B ( MGI:3045296)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008468. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008468_01	euxassay_008468_02	euxassay_008468_03	euxassay_008468_04

euxassay_008468_05	euxassay_008468_06	euxassay_008468_07	euxassay_008468_08	euxassay_008468_09

euxassay_008468_10	euxassay_008468_11	euxassay_008468_12	euxassay_008468_13	euxassay_008468_14

euxassay_008468_15	euxassay_008468_16	euxassay_008468_17	euxassay_008468_18	euxassay_008468_19

euxassay_008468_20	euxassay_008468_21	euxassay_008468_22	euxassay_008468_23	euxassay_008468_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12134_wholemount_strong.wlz

12134_wholemount_moderate.wlz

12134_wholemount_weak.wlz

12134_wholemount_possible.wlz

12134_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12134_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

weak

regional

weak expression: see section 11 12 13 14

choroid invagination

weak

regional

weak expression: see section 05 06 07 08 09 10 15 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35648
Entity Detected:	Efhb, EF hand domain family, member B ( MGI:3045296)
Sequence:	sense strand is shown >T35648 CCTGCTGGAGCAGAAAGAGTATTTTATGGCAGAGCAAATGACCCCCAAATTGCACCTTATTTGACACATG GACTAAGATCTAAAATCTCAATACCGATGGGATCATTGATAAACCCACAGCCCATTACCACGTTTCAACA GAAAATTAAAGACAAAAAAGAATCTATATATTTTAGCCATCAACGAGCACCATTGGGAAAATCTCATGAT CAAACACCAGGATTGCCAAAAGGAATGGATGTGATCAACACAACATTAGGGACACCGACTATCAGAGAAC TGTCTGTTCGTGACACTGTGAATCCATCGAAATCCTTCGAAGATGTGCTTAAAGAAGGACAGGAAGGACA TGATTTGTACACTGTCTCTCACAATGATTATTTTGCAGGAGAAGCAAAGAATCGAAAATATAACCCGGCA AGCTTCCATAGGTTTAACTTGTATGGAATCCCAACACCTCACTTTAATGATGGGCGCACCATGGCTAAAG CTTTACATTGGCTCCATGAACTACAAATGGAAAGAGGAGCGAAAATTGTATCCAAAAGAGTTGATGACTT CAAAGAGAAATTTCAACACAAACTTGGAAAAGTTTTAGACCCCATTGCAGAAACTATGAATGTTCCCCCA GGTCACACATTTGGAAGTTGCCTCCATCCTGAGGAATATGGAGCTGGCGATCTCATCCATTATAGAAGCC CAGATGAATATCTTCGTGGGAAGGACCACCAGAGAGCCGTGGTTGCAGCTGCTCGCCACCACTTGAAGAA ATTTAATCACCAGAACTTTGACACTTTGCAGGTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67197. Forward Primer - name:067197_F_cDNA_4921525D22Rik, sequence:CCTGCTGGAGCAGAAAGAGTAT; Reverse Primer - name:067197_N_SP6_cDNA_4921525D22Rik, sequence:CCACCTGCAAAGTGTCAAAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12130 same embryo
	EMAGE:12132 same embryo
	EMAGE:12129 same embryo
	EMAGE:12131 same embryo
	EMAGE:12133 same embryo
	EurExpress:euxassay_008468 same experiment
	MGI:4824481 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source