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EMAGE:12142

Rtn3 reticulon 3 ( MGI:1339970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142
"Pseudo-wholemount" of euxassay_008415. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008415_01 euxassay_008415_02 euxassay_008415_03 euxassay_008415_04
EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142
euxassay_008415_05 euxassay_008415_06 euxassay_008415_07 euxassay_008415_08 euxassay_008415_09
EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142
euxassay_008415_10 euxassay_008415_11 euxassay_008415_12 euxassay_008415_13 euxassay_008415_14
EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142
euxassay_008415_15 euxassay_008415_16 euxassay_008415_17 euxassay_008415_18 euxassay_008415_19
EMAGE:12142 EMAGE:12142 EMAGE:12142 EMAGE:12142
euxassay_008415_20 euxassay_008415_21 euxassay_008415_22 euxassay_008415_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12142Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12142_wholemount_strong.wlz
12142_wholemount_moderate.wlz
12142_wholemount_weak.wlz
12142_wholemount_possible.wlz
12142_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12142_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 09 10 11 21 22 23 moderate expression: see section 06 08
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 20 21 22 23
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 08 09 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23
vagus x ganglion
strong strong
regionalstrong expression: see section 09 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 09 10 18 19 20
trigeminal v nerve
strong strong
regionalstrong expression: see section 11 12 18
spinal cord
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 11 12 13 17
cervical ganglion
strong strong
regionalstrong expression: see section 10 11 17 18
thoracic ganglion
strong strong
regionalstrong expression: see section 13 14 15 16
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 12 17 18
neural retina
strong strong
regionalstrong expression: see section 01 02 03 04 05 06
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 17 18 19 20 21
tongue
strong strong
spottedstrong expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9283
Entity Detected:Rtn3, reticulon 3 ( MGI:1339970)
Sequence:sense strand is shown

>T9283
TTCCGAACAGGCGTCGCCGGGAACTTCGGAGAGCCAGCTGCTGGATTATTCTGTGTCCCCTCCCACTCTC
CCGCCCTACGTCTCTCTTCACCCTTCTCCCGCCCTTGCTCGTACAGCCATGGCGGAGTCGTCAGCGGCCA
CTCAGTCCCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAGCTCTCGGCGGCGGCGGCGGGAG
CCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCGTGTGCGGATTCCTTTGTTTCTTCC
TCTTCCTCTCAGCCTGTATCTATATTTTCGACCTCACAAGCGGGATTGAGCTCTCTTTGCTCTGATGAGC
CACCTTCAAAAAGTATGACTTCCTCCTTTCTTTCATCTTCTGAAATACATAACCCTGACCCTACAACACC
GCTTGGAGAAAAGAGTGAAACATTAGGTAGCCAGTTTGTTCTAGCTAAAGGAAAAGACCCCTTGGTTCTT
CTAGATAAGAAAAAACTGGACTCACCTCAGGGGACCAACAAGGACAGAGTAGACGCTCCGGTTTCTCTTG
CAACTGGCATTCCTTGCAGCCACCCTTCTATTCCAGACAGTTTCCCAGAGCAACCTGCTTTTCTGTCAAA
AGAAATTGGTCCAGCAGAAGAGTGGGTAGTTAAAGACCAAGAACCCAAGAACCCAAACAAGGTTCCAGAT
GGGGAGGACAGAAGTGCACTGGATTTTGGGCAGTCTAAGGCAGAACACATTTGTACATATTCCTTGTCCC
CATCTGAACTTCCAGTAGCCAGTGTAGAAAAAGATTCTCCTGAGTCACCGTTTGAAGTAATTATTGACAA
AGCAACATTTGACAGAGAATTTAAAGATTTGTATAAGGAAAACCCAAATGATTTGGGTGGCTGGGCAGCT
CATGGCGATAGAGAATCCCCCGCAGACTTGTTGGAAATGAATGACAAACTCTTTCCACTGAGAAATAAAG
AGGCAGGGCGTTACCCATCCTCTGTACTGCTCGGTAGACAGTTCTCACACACTACAGCAGCACTGGAAGA
GGTGTCTAGATGTGTGAATGATATGCATAACTTTACTAATGAAATACTGACTTGGGACTTAGATCCCCAA
GCAAAACAACAGGCCAATAAAACATCTTGCACCACAGAAAGTACAGGACTAGACAGGAGTGAACTTCGCT
CAGAAATTCCAGTTATAAATCTTAAAACAAACCCTCAACAGAAAATGCCTGTATGTTCTTTTAATGGGAG
CACTCCCATTACCAAATCCACAGGTGACTGGACAGAAGCATTTACAGAAGGAAAACCTGTAAGAGACTAC
CTCAGTTCCACAAAAGAAGCTGGTGGCAACGGTGTGCCAGGCAGTTCTCAGCTTCATTCTGAGCTGCCTG
GCTCTATGCCTGAGAAATGGGTCTCAGGCTCTGGAGCAGCCACAGTGGAAGTAACTTTACCTAACCTGAG
GGGTGCCTGGCCTAACTCTGTGATGGGGGAAGTCACAGAGGTTGATAGTTCTGGGGAATCTGATGACACA
GTAATAGAGGACATCACAGAAAAACCTGACTCCCTTCCAAGTGCTGCTGCAAAAACAAGTGAAAGGGAAA
TCAAAGAGACTCCCAGTCGTGAAACTGTGAGGAGTGAAATGTGTGAAAACTCTGAGCAGCCGCAGGCCCA
GCCAGAAACTCCTACTCAGAAGAGTCTGGAAGGTGAGGTGGCTTCACAAGTACCTAATACCCTGAATGAA
GTCACACCTGAAAAGCTTGATATGACTAACAACCCCAAAGTTTGCTCAGCAGCACCTCCAAGTGTTCTTA
ATGAGACAGGATTCTCACTAACTGTGCCAGCTTCTGCCAAGTTGGAATCTTTGCTTGGAAAATATGTTGA
AGATACAGATGGTTCCTCCCCAGAGGACTTGATGGCTGTCCTCACAGGAGCCGAGGAGAAGGGGATAGTG
GATAAAGAGGAAGGTGATGTTTTGGAAGCAGTGTTAGAGAAGATAGCAGACTTTAAAAACACTTTGCCTG
TGGAACTCTTGCATGAAAGTGAGCTAAGTGGTTCTGAAACCAAAAATATTAAAAGCAAATACAGTGAAGA
CAGCAGAGAAACAACTGGAGGTGCCCCTACGATGTCTCCCGACTTAGAGCAGGAGCAGCTCACCATCAGA
GCCATTAAAGAATTAGGAGAAAGGCAAGCTGAGAAGGTGCAGGATGAAGGAATATCTTCTGGAGGAAAAC
TCAAGCAAACCTTTGCTCCACAGTCTGGGCCACAGAGTTCATCTGACATCCTAGAACACACAGATGTCAA
AACTGGATCTGATCTTGGAATTCCCAAAAATCCTACTATCATCAAAAACACTAGAATAGATTCTATTTCC
AGCCTTACCAAGACTGAAATGGTTAACAAGAATGTTCTAGCAAGACTTCTCAGTGATTTCCCAGTGCACG
ATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACACTGATCATGCTGCTCTC
TCTGGCAGCCTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGCTCTTCTCTCTGTCACCATCAGC
TTCAGAGTCTATAAGTCTGTCATTCAAGCTGTGCAGAAGTCAGAAGAAGGACATCCATTCAAGGCCTACT
TGGATGTGGACATTACCCTGTCTTCAGAAGCTTTCCACAACTACATGAATGCTGCGATGGTGCATGTCAA
CAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGTTGACTCCTTGAAGCTGGCTGTC
TTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAATGGAATTACCCTTCTGATTCTCGCCGAGCTGC
TTGTCTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTGACCACTATGTTGGGATTGCCCG
GGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGGAATCGCCAAAAAAAAGGCAGAA
TAAGCACGTGAAGCAAGAAGTGCATCAGTTACTAAAACACCATTTAATAGTTATAACATCATCACTTGTA
CTATGAAGGAACTTGCTCAGTGTCAGCTAGAGTTTGGTTCCAAGTTGTTTGTTCATTTTAATTTGGTGTT
CTCTCCCCTCCTCTCCCTTTACCCTCAGTACCAAGCACAAGATGTGTTAGACTGAAAAAAGAACTGATCT
CTTAGAACCGTAAAGAATAAAGATTGAATCAGATTACTAGAATTAATCAGACCATAGTGGGGTGGAGCTT
TACATGTACCATAAAAGGAGAAAAAGAGAAGTGTCTCTAGGGTCCTCTCTCCAGTTCCAAGGACTCGTAT
ACAGCTCCCATTTATAATTTTGCCCTTATTTTTAAGTTAGGAAATAATGATTAACTTATGAATAAATAGT
ATGGGGCAAGAGTGGGATTTCTTCCGTTGGGTTTGTATGGCCCTCCAGTCCCACCTTCCTGCCTTACACA
TAAGCAGCTACTCTTACTGCTCCTCTTCTTCCTTAATGAAAGAACTCTCAGCTCTGAATAACTTACAGAA
CTCCCAGAATGCCACCTGAGAAATGGGGGTTGGGGGGACAGTGGGTGGAGCAGTCAGCAGGAGGGCAGCC
ACCACCCTCTCTGCGGCTGCTCCTAGGGAAGGAAGGGCTCTGGGGCTGGGAGGGCCGTGTACAGCCCTTG
GTCTGGGATCCATGTGTTGGTTGAGAAATCATTCCAGAGTGTGCTTTCCCCTCTCCCTGCCCACCTTACC
TCACGTTTAATAAATATGGTTGTACTTTTCTTATTATGAAATAAATGTCTGTAACTGCTGTACACTGCTA
TAAACTTGTTAAAGAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6839323 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839323 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12146 same embryo
 EMAGE:12144 same embryo
 EMAGE:12143 same embryo
 EMAGE:12145 same embryo
 EMAGE:12141 same embryo
 EurExpress:euxassay_008415 same experiment
 MGI:4827829 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS