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EMAGE:12144

Prlhr prolactin releasing hormone receptor ( MGI:2135956)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144
"Pseudo-wholemount" of euxassay_008477. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008477_01 euxassay_008477_02 euxassay_008477_03 euxassay_008477_04
EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144
euxassay_008477_05 euxassay_008477_06 euxassay_008477_07 euxassay_008477_08 euxassay_008477_09
EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144
euxassay_008477_10 euxassay_008477_11 euxassay_008477_12 euxassay_008477_13 euxassay_008477_14
EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144 EMAGE:12144
euxassay_008477_15 euxassay_008477_16 euxassay_008477_17 euxassay_008477_18 euxassay_008477_19
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euxassay_008477_20 euxassay_008477_21 euxassay_008477_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12144Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12144_wholemount_strong.wlz
12144_wholemount_moderate.wlz
12144_wholemount_weak.wlz
12144_wholemount_possible.wlz
12144_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12144_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35182
Entity Detected:Prlhr, prolactin releasing hormone receptor ( MGI:2135956)
Sequence:sense strand is shown

>T35182
AACAGGTAGCCATGACCTCACTGTCCACTGAGACCACTGGAGACCCCGATTTGTCTTCTGGGCTGTTGCC
AGCCAGCTCCACTCCAGCCAACCAGAGCGCAGAGGCCTCGGAGGGCAACCTGTCTGCGACGGTTCCCCGA
GCTGCAGCAGTCACGCCGTTCCAGAGCCTGCAGCTGGTGCACCAGCTGAAGGGGTTGATCGTGATGCTGT
ACAGCATTGTGGTGGTCGTGGGTCTGGTGGGCAACTGCCTGCTTGTGCTGGTGATCGCGCGCGTGCGCCG
GCTGCACAACGTGACCAACTTCCTCATTGGCAACCTGGCCTTGTCCGACGTGCTCATGTGTGCCGCCTGT
GTGCCTCTCACGCTGGCTTATGCCTTTGAACCTCGGGGCTGGGTGTTCGGTGGAGGCCTGTGTCATCTGG
TTTTCTTCCTGCAGCCGGTCACCGTCTATGTATCGGTGTTCACACTCACCACAATCGCCTTGGACCGCTA
TGTGGTTCTGGTGCACCCGCTACGTCGGCGCATTTCACTGAGGCTCAGCGCCTACGCGGTGCTGGGCATC
TGGGCTCTATCTGCAGTGCTGGCGCTGCCGGCCGCGGTGCACACCTACCATGTGGAGCTCAAGCCCCACG
ACGTGCGCCTCTGCGAGGAGTTCTGGGGCTCGCAGGAGCGCCAACGCCAGATCTACGCCTGGGGGCTGCT
TCTGGGCACCTATTTGCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96569. Forward Primer - name:096569_F_cDNA_Gpr10, sequence:AACAGGTAGCCATGACCTCACT; Reverse Primer - name:096569_N_SP6_cDNA_Gpr10, sequence:GAGCAAATAGGTGCCCAGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12142 same embryo
 EMAGE:12146 same embryo
 EMAGE:12143 same embryo
 EMAGE:12145 same embryo
 EMAGE:12141 same embryo
 EurExpress:euxassay_008477 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS