EMAGE mouse gene expression spatial database

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EMAGE:12150

Polr1b polymerase (RNA) I polypeptide B ( MGI:108014)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008540. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008540_01	euxassay_008540_02	euxassay_008540_03	euxassay_008540_04

euxassay_008540_05	euxassay_008540_06	euxassay_008540_07	euxassay_008540_08	euxassay_008540_09

euxassay_008540_10	euxassay_008540_11	euxassay_008540_12	euxassay_008540_13	euxassay_008540_14

euxassay_008540_15	euxassay_008540_16	euxassay_008540_17	euxassay_008540_18	euxassay_008540_19

euxassay_008540_20	euxassay_008540_21	euxassay_008540_22	euxassay_008540_23	euxassay_008540_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12150_wholemount_strong.wlz

12150_wholemount_moderate.wlz

12150_wholemount_weak.wlz

12150_wholemount_possible.wlz

12150_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12150_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9484
Entity Detected:	Polr1b, polymerase (RNA) I polypeptide B ( MGI:108014)
Sequence:	sense strand is shown >T9484 CCGTCCGGGACTGGCAGAAGCATGGATGTCGACGGCCGGTGGCGGAACCTGCCCAGCGGGCCCAGCCTAA AGCATTTAACCGACCCCTCGTACGGGATTCCCCCAGAGCAGCAAAAGGCAGCTTTGCAGGACCTGACGCG GGCGCACGTGGACTCCTTCAACTACGCGGCGCTGGAGGGGCTGAGCCACGCGGTGCAGGCCATACCTCCC TTTGAATTTGCTTTCAAAGATGAGCGCATATCTCTTACCATTGTGGATGCTGTCATCAGTCCACCCTCAG TGCCCAAAGGGACCATCTGCAAAGATCTCAACGTTTATCCAGCTGAGTGTCGTGGCCGAAAGAGCACCTA CCGAGGGAGGCTGACGGCTGATATCAGCTGGGCCGTGAATGGAGTCCCCAAAGGGATCATTAAACAGTTT CTCGGCTATGTTCCCATCATGGTGAAGTCCAAGCTTTGCAACTTATACAACCTTCCTCCTCGAGTCCTCA TTGAGCACCATGAGGAGGCAGAGGAAATGGGCGGTTATTTTATAATCAATGGCATAGAAAAAGTCATCCG CATGTTGATTGTGCCCCGGAGAAATTTCCCCATCGCCATGGTAAGGCCGAAATGGAAAAGCAGAGGACTC GGCTACACTCAGTTTGGGGTCTCCATGCGCTGTGTGAGAGAAGAGCACTCCGCCGTGAACATGAACCTGC ACTATGTGGAGAACGGCACGGTTATGTTAAACTTTATTTACCGCAAAGAGCTCTTTTTCCTTCCTTTGGG ATTTGCACTTAAGGCACTCGTGAGCTTTTCTGATTATCAGATTTTCCAGGAGCTTATCAAGGGCAAAGAG GAGGACTCTTTCTTTAGGAATTCTGTTTCTCAGATGCTGCGGATTGTCATAGAAGAGGGCTGTCACTCAC AGAAGCAGGTTCTTAACTATCTGGGAGAATGCTTCCGAGTGAAGCTCAGCCTTCCTGATTGGTACCCTAA CGTGGAAGCTGCCGAGTTCCTGCTTAACCAGTGCATCTGCATCCACCTGCAGTCCAACACTGACAAGTTC TACCTGCTCTGCCTCATGACCCGGAAGCTGTTCGCTTTAGCCAGAGGGGAGTGCATGGACGACAACCCTG ATAGCCTAGTGAACCAAGAGGTCTTCAGCCCTGGGCAGCTCTTCCTGATGTTTCTGAAGGAAAAAATGGA AAACTGGCTAGTGTCTATTAAAATAGTTTTAGATAAGAGGGCTCAGAAGGCCAATGTTTCCATAAACAAC GAAAATTTGATGAAGATTTTTAGTATGGGAACAGAGCTAACAAGACCATTTGAATATCTTCTTGCTACTG GGAATCTGCGTTCTAAAACAGGTCTCGGCTTCCTGCAAGATTCTGGCCTGTGTGTTGTGGCTGACAAGCT GAACTTCCTTCGCTACCTCTCCCATTTCCGCTGTGTGCACAGAGGGGCTGCCTTTGCCAAGATGAGGACC ACCACCGTGCGCAGGCTGCTGCCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACTCCAGACGGGGCGC CTTGTGGGCTGTTGAACCACTTGACTGCCGTGTGTGAGGTTGTTACCAAGTTTGTGTACACGGCATCTAT TCCAGCCTTGCTCTGTGGCTTAGGAGTCACTCCTGTTGATACAGCGCCATGTCGACCGTACAGTGACTGC TACCCTGTCCTGCTGGATGGCGTCATGGTGGGCTGGGTGGATAAGGACCTTGCTCCTGAAGTTGCAGACA CTCTCCGTCGATTCAAGGTGTTGAGAGAAAAAAGAATTCCTCCCTGGATGGAGGTGGCCCTGATTCCCAT GACAGGAAAACCAAGTCTGTACCCAGGTCTGTTCCTCTTTACCACTCCCTGCAGGCTGGTGAGGCCTGTG CAGAACTTGGAACTGGGCAGAGAAGAGCTCATTGGAACCATGGAACAGCTCTTCATGAACGTCGCCATCT TTGAGGACGAGGTGTTTGGTGGCATCTCCACACACCAGGAGCTCTTCCCTCACAGCCTGCTGAGCGTGAT CGCCAACTTCATCCCCTTCTCGGATCACAACCAGAGTCCTCGGAACATGTACCAGTGCCAGATGGGTAAG CAGACCATGGGCTTTCCGCTGCTCACTTACCAAAACCGATCAGATAATAAACTCTATCGACTCCAAACCC CTCAGAGCCCTCTAGTGAGACCGTGCATGTATGATTTCTATGACATGGACAACTATCCGATCGGGACAAA CGCCATTGTAGCTGTGATCTCCTACACTGGCTATGATATGGAGGACGCCATGATTGTCAACAAGGCTTCC TGGGAACGAGGCTTCGCTCATGGAAGCGTCTACAAGTCTGAGTTCATAGACCTCTCAGAGAAGTTTAAGC AGGGAGAGGATAACCTGGTGTTCGGGGTCAAGCCTGGTGACCCTCGGGTGATGCAGAAGCTGGACGATGA TGGGTTGCCATTCATAGGCGCAAAGCTGGAGTATGGCGACCCTTACTACAGCTACCTCAACCTTAACACT GGGGAAGGCTTCGTGGTTTACTACAAGAGTAAAGAAAACTGTGTTGTGGACAACATCAAAGTGTGTAGTA ACGACATGGGCAGTGGGAAGTTCAAGTGTATCTGCATCACAGTCCGCATCCCCCGGAACCCAACCATTGG AGATAAGTTTGCCAGCCGACATGGTCAGAAGGGCATTTTGAGCAGATTGTGGCCAGCCGAGGACATGCCT TTCACTGAGAGTGGCATGATGCCGGACATTCTGTTTAATCCTCACGGGTTTCCCTCCCGTATGACCATAG GTATGTTAATCGAGAGCATGGCTGGGAAGTCTGCAGCTTTGCACGGTCTCTGCCATGATGCTACACCCTT CATCTTCTCCGAGGAGAACTCTGCCTTAGAGTACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAACTTC TACGGCACCGAGAGACTGTATAGTGGCATCAGTGGGATGGAGCTGGAGGCTGACATCTTCATTGGTGTGG TTTATTACCAGCGCTTACGACACATGGTGTCAGACAAATTTCAAGTCAGAACAACTGGAGCCAGGGACAA AGTCACCAACCAGCCCCTCGGGGGACGAAATGTTCAGGGTGGAATCCGCTTTGGGGAGATGGAGCGGGAT GCTCTGTTGGCACACGGTACGTCTTTCCTTCTTCATGACCGGCTTTTCAACTGCTCTGACCGTTCTGTCG CTCACATGTGTGTAGAGTGTGGCAGTTTGCTTTCTCCGCTACTTGAGAAGCCTCCTCCGTCTTGGTCTGC GATGCGCAACAGAAAGTACAACTGCACTGTCTGTGGCCGCAGTGACACCATCGACACCGTCTCTGTGCCG TATGTCTTCCGGTACTTTGTAGCTGAGCTGGCAGCCATGAACATCAAAGTGAAACTGGATGTCATCTGAC TTGATCCCCGCCATCTGAGCTAGGAGATAAGAACAGGAGGTGTCCTGTATAACACAAGTGAGGGTATTAT GGGCTCACTCTGGGTCTATACAAGAATTTCAGTACTGAAGCCAAGAACAGGCAGGTGAAAATTCAAGACT TCTGAGTCTCTGTAACCTACTGAAGTTGGTTTTCATATCTTTTTCAAGACAGGGTTTCTCTGTGTAGCCC TGGCTGTCCTGGAACTCACTTTTTAGACCAGGCTGGTCTCAAACTCAGAAATCCACCTGCCTCTGCCTCC TGAGTGCTGGGATTAAAGGCGTGCGCCACCACGCCCAGCCTCATATCTTTTTTTAAAAAAGTTATTTTTG TGCCTTTAAAAAATGACTTAATTGATAATAGATCATGCAGGGCAGGGCGCCTTCTTTCTGGGCCCAGGTT CACTGCTCCCTGCTTTCAGTAGCAGAGGGTGTCTGTTATCCAGAGCAGGCCAGTACAATAAACAGAAAAT GGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30355130 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30355130 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12152 same embryo
	EMAGE:12155 same embryo
	EMAGE:12151 same embryo
	EMAGE:12153 same embryo
	EMAGE:12154 same embryo
	EurExpress:euxassay_008540 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source