EMAGE mouse gene expression spatial database

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EMAGE:12153

Fhod1 formin homology 2 domain containing 1 ( MGI:2679008)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008535. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008535_01	euxassay_008535_02	euxassay_008535_03	euxassay_008535_04

euxassay_008535_05	euxassay_008535_06	euxassay_008535_07	euxassay_008535_08	euxassay_008535_09

euxassay_008535_10	euxassay_008535_11	euxassay_008535_12	euxassay_008535_13	euxassay_008535_14

euxassay_008535_15	euxassay_008535_16	euxassay_008535_17	euxassay_008535_18	euxassay_008535_19

euxassay_008535_20	euxassay_008535_21	euxassay_008535_22	euxassay_008535_23	euxassay_008535_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12153_wholemount_strong.wlz

12153_wholemount_moderate.wlz

12153_wholemount_weak.wlz

12153_wholemount_possible.wlz

12153_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12153_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 15 16 weak expression: see section 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 20 21 weak expression: see section 10 18 19

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 19 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 10 18

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 17 18

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 13 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19

neural retina

weak

regional

weak expression: see section 01 02 03

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18 19 weak expression: see section 08 09 20

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 11 18 19 20 21 22 23

trachea

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9483
Entity Detected:	Fhod1, formin homology 2 domain containing 1 ( MGI:2679008)
Sequence:	sense strand is shown >T9483 CCCAGCCTGGCCCGGCCCTAACAGCAAAGTTTAGTCGAGTTGAAGTTGCAGCATTTGCAGGGGACACAAG AGGCGTCCAGCGGTAGCCGGTGGCAGAGCCATGGCGGGCGAGGAAGAGCGCGGGGATGGGGACCCAGTAT CCGTGGTAACTGTGAGAGTGCAGTACCTGGAAGACACCGACCCTTTCGCTTGTGCCAACTTCCCGGAACC ACGCCGGGCCCCCACCTGCAGCCTGGACGGGGCTCTGCCCCTGAGTGCGCAGATCCCTGCTCTGCACCGA CTCCTGGGGGCGCCTCTTAAGCTGGAGGACTGTGCATTGCAAGTGTCTCCCTCCGGATACTACCTGGACC CGGAGCTGTCCCTAGAAGAACAGCGGGAGATGCTGGAGGGTTTCTATGAAGAGATCAGCAAAGGGCGGAA GCCCACGTTAATCCTGCGGACCCAGCTCTCCGTGAGGGTCAATGCTATCTTGGAAAAGCTGTATGGCTCC AGTGGCCCCGAGCTCCGCCGATCTCTCTTCTCATTAAAGCAGATATTCCAGGAAGACAAGGACCTGGTGC CCGAATTCGTGCACTCGGAGGGTCTGAGTTGCCTGATCCGCGTGGGTGCTGCTGCAGACCACAACTACCA GAGCTACATCCTTCGAGCCCTGGGCCAGCTGATGCTGTTTGTGGATGGGATGCTGGGGGTGGTGGCCCAC AGCGAGACCGTGCAGTGGCTGTATACCCTGTGTGCTAGCCTGTCCCGCTTGGTGGTAAAGACAGCCCTGA AGCTGCTGCTGGTGTTTGTGGAGTATTCCGAGAACAACGCGCCGCTGTTCATCCAGGCAGTCAACGCAGT AGCCAGTGCCACCGGTACTCTTCCCTGGGCCAACTTGGTGTCCATCCTGGAGGAGAAGAATGGAGCTGAT GCAGAGTTGTTGGTGTACACTGTCACTCTCATTAACAAGACGCTGGCGGCACTCCCAGACCAGGACTCCT TCTATGATGTGACAGATGCTCTGGAGCAGCAGGGCATGGAAGCGCTGGTCCAACGTTTCTTGGGCACCGC TGGCACTGATGTTGACCTGCGAACCCAGCTAACGCTCTATGAGAGTGCCCTTCGGTTAGAGGATGGAGAT ATGGAAGAGGCTGCAGCCGCCGCTGCTGCAGGTGGCCGGCGAGAGCGGCGGAAGCCATCCTCGGAGGAGG GCAAAAGGAGCCGAAGATCACTAGAAGGTGGAGGCTGCCCTGTGCGTGCCCCAGAACCTGGCTCTACAGG CTCCGCCTCACCAGTAGGCTCCACCCCCTCCACTGGCTCCGCCCCGCCTACAAATCCAGCCTTCAGCTCT ACTGGCCCAGCCTCTGGCCTTCTTCGAACCTCAGTGAACCTCTTTCCTACCATTTCCGTGGGGCCGTCAG TGGACAGTTCCTGTGAGAGAAGCGTCTACAAAGCCCGGTTCCTGGAGAATGTGGCGGCAGCAGAGACGGA GAAGCAGGCTGCTCTGGCCCAAGGCCGAGCGGAGACGCTGGCTGGAGCCACGGTAGATGACACTGATGGA TCATCAGGCACAAGGGAACTGTGGGACTCCCCAGAGCCAGCCTCTGCACCCAGGACACCCCAGAGCCCTG TTTCCCGAATCCTGCTGCGCACCCAGCGGAGTCTTGAGCCAGAGCCCAAGAAGCCAGTGTCACCACCAAG CCCTAAGGCTGAGCCTATCCAGGAGCCTCCCACCTGTGTCCCCAAGCTCTGCATTGGGGACTTGGACTTC TCAGACCTAGGGGAGGATGAAGACCAGGACACACTGAATGTGGAATCTGTGGAGGCTGGAAAAGCATCTC CCTTCCTGTCATCTCTATCGCCCTCACTCTCTGGGGGTCCCCCTCCTCCGCCCCCACCTCCTCCACCCAT CACAGGCTCCTGCCCCCCACCTCCGCCCCCACCTCTCCCCCCACCCGCCACAGGCTCCTGCCCTCCACCT CCGCCCCCACCTCCCCCACCCATCATAGGCTCCTGCCCACCGCCTCCACCCCTGGCTGCTCCTTTTACCC ACTCAGCACTTGACGGCCCAAGGCACCCCACCAAAAGGAAGACAGTAAAACTTTTCTGGCGGGAACTAAA GCTGACTGGGGGCCCTGGGTGCTCTAGAAGCCGCTTTGGGCCTTGTCCTACCCTGTGGGCCTCGCTGGAA CCCGTCTCGGTGGACACAGCCCGCCTGGAACACCTATTTGAGTCCAGAGCCAAGGATGTGCTACCAACCA AGAAAGCTGGTGAGGGCCGCCGGACAATGACCGTAGTGCTGGACCCCAAGCGCAGCAATGCCATCAACAT TGGCCTAACCACTCTGCCACCCGTGCACGTCATCAAGGCTGCCCTGCTCAACTTCGATGAGTTTGCTGTC AGCAAAGATGGCATTGAGAAACTGCTGACAATGATGCCCACGGAGGAAGAGCGGCAGAAGATTGAGGAAG CCCAGCTGGCTAACCCCGATGTACCCCTCGGCCCCGCTGAGAATTTCCTGATGACGCTTGCTTCCATTGG AGGCCTGGCTGCGCGCCTACAGCTCTGGGCTTTCAAGCTGGACTATGAAAGCATGGAGCGGGAAATTGCA GAGCCACTGTTTGATCTGAAAGTGGGCATGGAACAGCTGGTACACAATGCCACCTTCCGCTGTATTCTGG CTACCCTTTTGGCTGTGGGCAACTTCCTCAATGGTTCCCAGAGCAGTGGCTTTGAGCTGAGCTACCTGGA GAAGGTGTCAGAAGTGAAGGACACAGTGCGACGGCAGTCATTGCTCTATCATCTCTGCTCCCTGGTGCTC CAGACCCGACCTGATTCCTCTGACCTCTACTCAGAAATTCCTGCCCTCACCCGCTGTGCCAAGGTGGACT TTGAACAGCTGACTGAGAACCTAGGGCAGCTGGAGTGCCGGAGCCAGGCTGCCGAGGACAGCCTCCGGAG CTTGGCTAAGCACGAGCTCTCCCCAGCTCTGCGTGCTCGCCTCACCCACTTCTTGGCCCAGTGTACCCGC CGGGTAGCCATGTTAAGAGTAGTGCATCGCCGAGTCTGCAATAGGTTCCATGCCTTCCTGCTCTACCTGG GCTACACCCCACAGGCAGCAAGGGATGTACGCATCATGCAGTTCTGCCACACACTGAGAGAGTTTGCCCT TGAGTATCGGACTTGTCGGGAACGGGTACTGCAGCAGCAGCAGAAGCGGGCTACATACCGTGAGCGCAAC AAGACCCGTGGTCGCATGATTACCGAGACAGAGAAGTTCTCAGGTGTGGCTGGGGAGGCCCCCAATAACC TGTCTGTCCCAGTGGCTGTGGGCAGCGGGCCAGGTCAGGGTGATACTGACAATCATGCCAGCATGAAGAG CCTGCTTACCAGCAGGCCGGAAGATGCCACACACAGCCGACGCAGCAGAGGTATGGTCCAGAGCAGTTCC CCCGTCTCACACACAGCAGTGGGGCCCTCCGCTGCATCCCCTGAGGAGACTGCAGCCTCCGGCTTACCCA CCGACACGTCAGATGAGATAATGGACCTGCTGGTGCAGTCAGTTACCAAGAGCGGTCCTAGAGCCTTAGC TGCTCGGGAGAGGAAGCGCTCTCGTGGCAACCGAAAGTCCTTGAGACGGACACTGAAGAGTGGACTTGGA GATGACCTGGTGCAGGCACTGGGACTAAGCAAAGCTCCTGGTCTAGAGGTGTGAGGTGCTACCACGCCTG GACATGACAGCGACATGCACAAGAGATGGAGTGCGGAAAGGGCCTGGAACAGAAACCTGGTTTCAGAAGC TTATACCCAGAAACCAGTATTGTGAACAGTATTAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTATACACGTACATGTATTTTGCATGTGTGAGTTCCTTATTTGGAACAAAATAAAGTTTACCTAG CTAATCTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30354865 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30354865 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12152 same embryo
	EMAGE:12155 same embryo
	EMAGE:12151 same embryo
	EMAGE:12150 same embryo
	EMAGE:12154 same embryo
	EurExpress:euxassay_008535 same experiment
	MGI:4824858 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source