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EMAGE:12154

Trmu tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase ( MGI:1919276)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154
"Pseudo-wholemount" of euxassay_008544. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008544_01 euxassay_008544_02 euxassay_008544_03 euxassay_008544_04
EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154
euxassay_008544_05 euxassay_008544_06 euxassay_008544_07 euxassay_008544_08 euxassay_008544_09
EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154
euxassay_008544_10 euxassay_008544_11 euxassay_008544_12 euxassay_008544_13 euxassay_008544_14
EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154
euxassay_008544_15 euxassay_008544_16 euxassay_008544_17 euxassay_008544_18 euxassay_008544_19
EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154 EMAGE:12154
euxassay_008544_20 euxassay_008544_21 euxassay_008544_22 euxassay_008544_23 euxassay_008544_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12154Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12154_wholemount_strong.wlz
12154_wholemount_moderate.wlz
12154_wholemount_weak.wlz
12154_wholemount_possible.wlz
12154_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12154_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9942
Entity Detected:Trmu, tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase ( MGI:1919276)
Sequence:sense strand is shown

>T9942
AGCTTGGACGCAGCCAGAAAGCTCGCGGTGTAGCCTGATGTCGGCGCTGCGGCACGTCGTGTGCGCCCTG
TCTGGCGGTGTGGACAGCGCGGTGGCGGCGCTGCTGTTGCGGCGGAGAGGTTACCAGGTGACAGGGGTAT
TTATGAAGAACTGGGACTCGCTGGATGAGCAGGGCGTTTGTGCTGCTGACAAGGACTGTGAAGATGCGTA
TAAAGTTTGTCAGATCTTAGACATCCCTTTCCACCAGGTGTCCTATGTGAAGGAGTATTGGAATGATGTG
TTCAGTGACTTTTTGAACGAGTATGAGAAAGGAAGGACTCCAAACCCCGACATCAACTGCAATAAGCATA
TCAAGTTCAGTTGCTTTTATCACTATGCTGTGGACAATCTTGGAGCAGATGCCGTTGCCACTGGCCACTA
CGCCAGGACCTCACTGGAGGATGAGGAAGTCTTTGAGCAAAAGCACACTAAGAAGCCTGACGGGCTGTTC
AGAAATCGATTTGAAGTCAGAAACCCCGTAAAACTTCTCCAAGCAGCTGACAGCTTTAAAGACCAGACCT
TCTTTCTCAGCCAGGTTTCCCAGGATGCCCTGAGAAGGACCATCTTTCCCCTGGGGGAACTAACAAAAGA
TTTTGTAAAGAAAATAGCCGCGGAGAATAGCCTCCATCACGTGCTTCAGAAGAGAGAGAGCATGGGCATC
TGCTTCATTGGTAAGAGAAACTTGGAGCATTTCCTTCTTCAGTATTTGCAGCCTCGGCCTGGAAAGTTTG
TTTCTATCGAGGATAATACAGTTCTGGGAACACACAAAGGTTGGTTCCTGTACACTTTGGGCCAGAGAGC
TAAGATCAGCGGCTTGAGAGAGCCCTGGTACGTGGTGGAGAAGGATGGCACCAAGGGTGACGTGCTTGTG
GCTCCCCGGGTGGACCACCCAGCTCTGTACAGGGACCTGCTCAGGACCAACCGAGTACACTGGATTGCGG
AGGAGCCACCTGCTGCCTTGGTCAGAGACAAGATGATGGAGTGCCATTTCCGATTCCGCCACCAGATGGC
ACTAGTGCCCTGCGTGCTGACTCTCAATCAGGATGGCACTGTGTGGGTAACGGCTGTGAAGGCTGTGAGG
GGCCTTGCCCTGGGACAGTTTGCAGTGTTCTACAAGGGTGAGGAGTGCCTCGGCAGTGGGAAAATCCTGC
GGCTAGGGCCGTCTGCCTACACACTCCAGAAGGGTAAGAACAGAACTCGAGTGGCTCCCGAGGCCTCCAG
TGACAGCCCAGGCCTGCACCCCACATCCTGACAAAGGCCAAGGAGGAGGCATTAGAGCAGAGTCGTCAGT
GATGGGTGTTAGTGCTGCCAGAATCAATAGGCGGGCTGGCTGCAGAGGAAGGAGGGTCTACGCTGCTGCT
CCAGGAAACCAGAGCGTGTGCTGGCTGTGGGCCCTGATGCAGCTGCTCTTCCACAGCATCCAGGAGGCCA
ATGGAGAAACCGCATCAAATAAAACTGTCAAAGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30305575 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30305575 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12152 same embryo
 EMAGE:12155 same embryo
 EMAGE:12151 same embryo
 EMAGE:12153 same embryo
 EMAGE:12150 same embryo
 EurExpress:euxassay_008544 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS