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EMAGE:12155

Rictor RPTOR independent companion of MTOR, complex 2 ( MGI:1926007)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155
"Pseudo-wholemount" of euxassay_008438. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008438_01 euxassay_008438_02 euxassay_008438_03 euxassay_008438_04
EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155
euxassay_008438_05 euxassay_008438_06 euxassay_008438_07 euxassay_008438_08 euxassay_008438_09
EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155
euxassay_008438_10 euxassay_008438_11 euxassay_008438_12 euxassay_008438_13 euxassay_008438_14
EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155
euxassay_008438_15 euxassay_008438_16 euxassay_008438_17 euxassay_008438_18 euxassay_008438_19
EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155 EMAGE:12155
euxassay_008438_20 euxassay_008438_21 euxassay_008438_22 euxassay_008438_23 euxassay_008438_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12155Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12155_wholemount_strong.wlz
12155_wholemount_moderate.wlz
12155_wholemount_weak.wlz
12155_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12155_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35641
Entity Detected:Rictor, RPTOR independent companion of MTOR, complex 2 ( MGI:1926007)
Sequence:sense strand is shown

>T35641
AAGACAGTGTCCCCTGACCTAAGTGTTTCTAATAAAGAGTAAGTGTAGAGACATCCCACAAGACAGCAGC
AGTGCAAGGCTCTTAGGAAGTGTGTGCACTGTTGCTGTGGTGATGTATCCAAGTCACCAGGAGCCGTGTC
CTCAGGAGCTTTCGGCTCTTGTCCAGAGCTTGAGACAGTTGAAGCTGGGTTCCATCACTGAGACCAGATG
TGCGTAGGGGCTGCTAGTGTGTGTGTAAACTGCCCACTGCTGTTTCCATGCACTCTCAGCCCCTTCAGCT
GATGCCCCTGGTGCCCCACTCAAAGCACAGACCATTAAGCAATAAACAAAATTGTCATTCAAATTATAAT
TGTGGTTATTCTCCTGTGATAAGAGGTGCTAATTTTGTGTGAGAAGAACATGTGACCTCTTTGGAAATAT
AGTTTGGAAGTAAAGTTTTTCCCCCTCTTCTTGTACTTCCATTTTGTCCCAAATTCACAGTTCATGAAAG
AAAAGTAAAATTGTTTAGGAGACTTCTAAGAAGAGCTGCTTGCAATAAACATTTCAGATTTTCCCGAGTG
TGAGTTGGGAAGGTCTCCATTTCCTCCATACTGACAAAGCCTGTTGTTCCAACGTCTCTTAGCTTGGAGA
GTGTCTCTACACTGAGTCTCACCTCAGCCTGCCAGTGACCCTGAGGTCACTGCTGAGGAAGACACAGGAG
TCTGCACTAGTCTTTACAAGAGCCGTTGTCATTTCAGGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73102. Forward Primer - name:073102_F_cDNA_4921505C17Rik, sequence:AAGACAGTGTCCCCTGACCTAA; Reverse Primer - name:073102_N_SP6_cDNA_4921505C17Rik, sequence:GACCTGAAATGACAACGGCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12152 same embryo
 EMAGE:12151 same embryo
 EMAGE:12153 same embryo
 EMAGE:12150 same embryo
 EMAGE:12154 same embryo
 EurExpress:euxassay_008438 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS