EMAGE mouse gene expression spatial database

EMAGE:12164

Rpl34 ribosomal protein L34 ( MGI:1915686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007041. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007041_01	euxassay_007041_02	euxassay_007041_03	euxassay_007041_04

euxassay_007041_05	euxassay_007041_06	euxassay_007041_07	euxassay_007041_08	euxassay_007041_09

euxassay_007041_10	euxassay_007041_11	euxassay_007041_12	euxassay_007041_13	euxassay_007041_14

euxassay_007041_15	euxassay_007041_16	euxassay_007041_17	euxassay_007041_18	euxassay_007041_19

euxassay_007041_20	euxassay_007041_21	euxassay_007041_22	euxassay_007041_23	euxassay_007041_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8385
Entity Detected:	Rpl34, ribosomal protein L34 ( MGI:1915686)
Sequence:	sense strand is shown >T8385 CCTTCTTCTGCTCTGTGTTTTTTTACCGTTTACGATCCGTAGCCATCCGAGGCGTCAGGCGGGCGGGACG CCCTTGGGGTCGGGACTGTCGCTGGGGTCGGTGGTGAGGGACGAGGAGAGGTGCGTACCGGCCGAGGCCT CGGCTCGTAGACTCGGGAAGCCCGAGATCAAACCCGGCTTCCGAAGCGGCACTCAGGATGGTCCAGCGTT TGACATACCGCCGTAGGCTTTCCTACAACACAGCCTCTAACAAAACTAGGCTGTCTCGAACCCCTGGCAA CAGGATTGTTTACCTCTACACCAAGAAGGTTGGGAAAGCACCTAAATCTGCATGTGGCGTGTGCCCAGGC AGACTTCGAGGGGTTCGTGCTGTGCGGCCCAAAGTCCTTATGAGACTGTCTAAGACACAGAAGCACGTCA GCAGGGCCTATGGCGGCTCCATGTGTGCCAAGTGTGTCCGTGACAGGATCAAGCGGGCTTTCCTTATTGA GGAGCAGAAAATCGTTGTGAAAGTCTTGAAGGCACAAGCACAGAGTCAGAAAGCAAAATAGATATGCACA TTTTTTAAGTAATAAAAATCAAGACTTGATCTACGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5683990 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5683990 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12166 same embryo
	EMAGE:12163 same embryo
	EMAGE:12167 same embryo
	EMAGE:12162 same embryo
	EMAGE:12165 same embryo
	EurExpress:euxassay_007041 same experiment
	MGI:4827798 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source