EMAGE mouse gene expression spatial database

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EMAGE:12166

9430031J16Rik RIKEN cDNA 9430031J16 gene ( MGI:2443135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007004. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007004_01	euxassay_007004_02	euxassay_007004_03	euxassay_007004_04

euxassay_007004_05	euxassay_007004_06	euxassay_007004_07	euxassay_007004_08	euxassay_007004_09

euxassay_007004_10	euxassay_007004_11	euxassay_007004_12	euxassay_007004_13	euxassay_007004_14

euxassay_007004_15	euxassay_007004_16	euxassay_007004_17	euxassay_007004_18	euxassay_007004_19

euxassay_007004_20	euxassay_007004_21	euxassay_007004_22	euxassay_007004_23	euxassay_007004_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12166_wholemount_strong.wlz

12166_wholemount_moderate.wlz

12166_wholemount_weak.wlz

12166_wholemount_possible.wlz

12166_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12166_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9407
Entity Detected:	9430031J16Rik, RIKEN cDNA 9430031J16 gene ( MGI:2443135)
Sequence:	sense strand is shown >T9407 GCAAGGAGAAAGAGCATCTCTGGGGCTGAAAGGAGACATTTAGAGAGGATGTGTCTGATTTTTGTTTCCA TTTATTTTTTTTCTTCACAGAACATGATCAAATGAGGTACCTTAGCCAGCTTGGTGACTGCTTCGGGTCT TGTAATCATCTGTGTAGCAAAGTGGGTAAAATCATTCAGTTTCGTCCCTGCTGGACCTGGAAGGAGAGGG GCTGTGCGCTCATCCCCACCCATTAGCTATGCCCTCTTTTCTCCGAATGTCGATTTAACGAAGCAAGCAC CTGTCTTCTGCCACTGCCTCTTCCTTGCAAACTTTAAAAGGCATTTTATCCGATAGAAAGAACAACGAAA ATGCTCTGATCTCTAGTGATGAAGAATCTTGGATTCGACACTTTTCACATCCAGATATAATGCACTTTAA TTGAAGAAATACTGAACTGAACCTACAAGTCTCTTTCTTATGGTGCTGGATTACTCCTCCCGAACTGCCC CGAGACCAGCCCTGAGAGAGGGCTCTGACAGACCCAACTCACCTCCTGACTATTCCACTTAGTTCTCCCC CGGGGACTTAATTTTTGAAAGCATCCTGTCTCCATGATACCATCCAAGATGATGAGTGCTAATCCTGAAG AAGACCCTCTGGACACCTTTTTCCAATATATTGAGGATATGGGAATGAAAGCATACGATGGCCTGGTTAT TCAAAATGCATCCGACATTGCTCGCGAGAATGATCGCCTGAGAAATGAGACAAACCTGGCTTACTTGAAG GAGAAGAACGAGAAACGTCGACGACAGGAAGAAACAATCAAACGCATAGGAGGAGAAGTGGGGCGAGGCC AGGACGCCAGTTACGCGGGCAAGCATTTCCGCATGGGGTTCATGACGATGCCTGCACCTCAGGACAGACT CCCACATCCCTGCTCCAGTGGCTTCACTGTGAGATCTCAGTCCCTGCACTCTGTAGGGGGCACTGAAGAT GACAGCAGCTGTGGATCACGAAGACAACCACCTCCCAAGCCCAAGCGAGACCCCAGCACTAAGCTTAGCA CTTCGTCTGAGACAGTCAACAGCACTGCAGCCAGTAAGAGTGGAAGATCTCTGGAGAGAGCAGAAGGTAA ATTCACCGTTCCTGCTTCCCACTCACCTCCAAGGGCTTCAACTTCAGGACACCTGTTTCCTTCCCCTGGG TCCCAGGAACGAAACATCAAGGTGTCTGCCAAGCCAAGACCCCACAGTGATGAATATTCAAAGAAAATTC CCCCTCCGAAACCAAAACGGAATCCTAATACTCAACTTAGCACGTCTTTCGATGAAACCTACATCAAAAA GCATGTGCCAAGGAGAACCTCTCTCCCGCGAGATTCATCCCTGTCCCAGGTTTGCAGCCCCGCGGCAGAC CCCGAAGAAGAGGAGCCTGTGTACATCGAGATGGTGGGGAACATCCTCAGAGACTTCAGGAAGGAAGAGG ATGACCAGAGCGAGGCTGTGTATGAGGAGATGAAATATCCCATTTTCGACGACCTGGGCCATGATTCCAA ATGTGACTTTGATCATCACAGTTGTTCTTCGCAGTGTGCTACTCCCACGGTGCCTGACCTGGACTTTGTC AAGTCTTCAGGACCATGTACTCCCAAGGGTCTGCTTTGTGACATACCCCCGCCCTTCCCCAACTTGCTTT CTCATAGACCACCCCTGCTGGTGTTCCCACCGGCCCCAGTACACTGCTCTCCCAATTCCGATGAGTCTCC ACTGACCCCTCTGGAGGTCACAAAGCTTCCTGTGTTGGAAAACGTGTCTTACATGAAACAGCCACCTGGA GCATGTCCATCCTCACTGCCATCTCATGGCTCCAGCCATGCTAAAGACCAGACAGGAGCCTTGGGTCCTG CTCCTGGAGCATCCATTCTCTCTTCTTCCCCTCCACCTCCATCCACTTTGTACAGAACCCAGTCTCCCCA TGGCTATCCAAAAAGTCACTCCACCTCCCCATCCCCTGTAAGCATGGGGAGGTCCCTGACGCCCTTGAGT CTCAAGAGGCCTCCCCCTTATGATGCTGTGCATTCTGGCAGCCTCTCAAGGAGTTCTTCCTCAGTGCCTC ACACGACCCCAAGACCCGTGTCACAGGATGGGGCCAAGATGGTCAATGCTGCGGTGAATACATACAGCGC TGCGCAGAGTGGGTCCAGGTCCAGGACACCCACCAGTCCTTTGGAAGAGCTCACCAGCCTCTTTACCTCA GGGCGGAGCCTGCTGCGGAAGTCATCCAGCGGCCGAAGGTCCAAAGAGCCAGCAGAGAAATCCACAGAGG AGCTGAAAGTTCGAAGCCACAGCACGGAGCCGTTACCAAAGTTGGACAGCAAAGAGAGAGGTCATTATGG ATCGTCTTCCTCTAGAGAGCCAGTGAAAGCTCAAGAATGGGATGGAACCCCGGGTCCACCTGTGGTCACC AGCAGAATGGGCAGATGCTCTGTGAGCCCCACACTGTTGGCAGGAAACCACAGCTCAGGTAAGCACGACC CTGTCCTTCCAAATGGCTCGAAAAGTCTAGAAGCTGCTCATATGCAGATATCACTTAGGAAACCATCACC TCAGCTCAGTCTACTTCCACCCAGACTGGCACCCCAGACACACACATTAAGGAGTAGACACATGAAGAAT GCCACGGTACCACTATTCATAGCGATGGCAAGGGAAACGATGCTTCTTGAAGCTTTGCTGTGCAGCAGTC TACAGTTAGGAAAGACTTTGTACCAAATGTCTTCAAAAATGACTACAGGCCTTAACTCAAGCCACTGCAA TTTATGAAAATCGCACAATTTAGGGGTGGTGGTCCTTTTTCTTAAAAAAAACAAGGCGACTTGAATAATT CAAAAAGAAGTAAAGCGGAGGATAGAAATATATCAATAATGATCTGAACGCATTAGAGGGAGAAGCTGGT TTCATTCACACAGTCACTGGTCATATGGCATGTATGAAGTTCTCAAATCCAAACCAAAGGGAAAATGCTA ATGTACCTTTCAAATTCACATCTTGAGATTGAAATACGCAATGTAATCCTGCATATTTAACAGATAAGTA TTATTAAACATAAAAAGTTTTAAAGCAGCATATGTTTGCTTTGATCTGATTCATCTACATGTTGGACTCA CAGAGAGCTGTCAATTAAAATAAACAGTGAGTAACCAAGAAACTTTAGGTTTCTGCTATCCAAAGAGTGA TAAGACCGTGAAGAAAAGACCAATCCTTTCATGTCGTTTTCCACTCTGATACTCATCTAGGAAAGGAATT TACACGCATTTAACCTATCTACTCTCTAACAAAGGGGAGTTAAAATATAGGAAGGCTGATGCTCTTTGTA CTTGGAGAGGGATATTTCTGGGAAAAAAAAACCCAGCCATAGCTTCTCAGATTTAGAATACTATGTAAAT CTTGATGGTAGAGCTATCCCATACACACTGTGAAGAACTCACCTTAAAGCACAAACTAAAAACTGACAGA AGAAAAGCTCTATACATTTAGCAGAAATTTTAAATGATATTTATGTTAAAGGAAAAATGGCCATGGGTAT GCATGATGGTTAGTCTTACTTGTCCACATGACACACTCTAGAATCATCCTGAAAGGGTGTCTCGAAGGGA CTATCTACACCAAGTTAGCTTGTGGTCATGCTTGTGGGAGATTGTCCTGACTCTGTTAGTTGATATGAGA AGACTCAGCCCACTGAGTAGCACCGTTCCTTAGCGTGGGGCCTGAGCAGGGTATGAGTAGATCAAGTGAG CTGAGCAGTCAGCACACATACACTCATTTTAACTGTGTCAATAGCTGCTTCAAGTTCCTACTAACCTGGT ATTGTGAGATAGATAAACCTTTTCCTCCCAAATTTGTTTTTAAGATGTTTGTAATAATAAAATTAGAACT ACCTGTGCATGCTGTCAAAAAAAAAAAAAAATTATGACTATCTTGTACAGTAGTTCAAACACAAAATATA TAAATAAGAAGAGTAGACAACTATGGCTAATGGCTAATTTGGAGCTTATCAGTATCTTATAGACATCTAA AATTGTATAATTTGTACCTAATATATGTGAATGATATATTTGGAACCCAGACATATTTTATAAAAACCGA AAATCTTATATGAAAATCTGTGATCCATTAGCCAATGTCTTAGGGCCAGGGATCAAAATGACCAATCAAA TATTCTTACATTGGTTATTAAAAATCTAGTGATATACAAGTAGATCATCGTAATGTAATGCAACTAATTT ATTTTAGTGGCTGTGTTCCAAAGAAACAGAACAAATAGTTATGCTTATATAGAAAGAGATTTATTATGAG AGATCTCTTCACTTCACCATGAAAGGTTAGAAGATCCAGATTAGTTCCTTGTAAGCAAATGTTCCAGGAA AGCCCATGGCATAGTGCAGGTCAAATAATGTCAGTGGTACAAACCACAGTCTGAGTTTAAAGGTTTCAAA AATTACATTGTTAATGTCCAAGGGTGGGAGAAAATAAATTATCCCAGCTCAAGAAGAGAGATAGGATATG TCCTTCTCTTTCTAGACCTTCACTGGATACATGGCGCTTGCTCACACTGGTGAGGATGATTTTCTGTTGA CTCAAATATCCATCCCTTGCAGAAAACACTCCCTTACAGATACACGTAGAAGTAATGCTTTGCCAGTTAT TTGAATATCCCTTAGCTCAGTTGATATAAAAATTAAGATGTGAAGTAGAAATCAGTTAATGTTTCCTGGG AGACTTTGGCTTTGGAAGGGTTAATCGGGTGTTTTGGTTTATATGAGAGTGATTTCTAGAGGCTCACCAG GATGAGAAGAAGGCAAATGAGATACTTGCTGGTGAAACCTGAAAATCAACAGGGTGAAAAAAAAATGTTT AGTGTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30355797 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30355797 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12163 same embryo
	EMAGE:12167 same embryo
	EMAGE:12164 same embryo
	EMAGE:12162 same embryo
	EMAGE:12165 same embryo
	EurExpress:euxassay_007004 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source