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EMAGE:12183

4933402P03Rik RIKEN cDNA 4933402P03 gene ( MGI:1918293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183
"Pseudo-wholemount" of euxassay_011174. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011174_01 euxassay_011174_02 euxassay_011174_03 euxassay_011174_04
EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183
euxassay_011174_05 euxassay_011174_06 euxassay_011174_07 euxassay_011174_08 euxassay_011174_09
EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183
euxassay_011174_10 euxassay_011174_11 euxassay_011174_12 euxassay_011174_13 euxassay_011174_14
EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183
euxassay_011174_15 euxassay_011174_16 euxassay_011174_17 euxassay_011174_18 euxassay_011174_19
EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183 EMAGE:12183
euxassay_011174_20 euxassay_011174_21 euxassay_011174_22 euxassay_011174_23 euxassay_011174_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12183Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12183_wholemount_strong.wlz
12183_wholemount_moderate.wlz
12183_wholemount_weak.wlz
12183_wholemount_possible.wlz
12183_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12183_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37413
Entity Detected:4933402P03Rik, RIKEN cDNA 4933402P03 gene ( MGI:1918293)
Sequence:sense strand is shown

>T37413
TAGTCCAGGTTACCGAATGTCCCCCCAAAGAGCCCCAACCAGCCAACGTCCAGGACGTCCACATCCACTG
TATCCTGGACCCTGTACAAGTGAAGATGGCCCAACCCACACAGTGTTCCTCTTCCTCTTCCCACTATTTT
TGCAAGCGCTCTAATGACAGGCGCAGCCGCAGGCGCTACGGCTACCAACAGGGCAACCTTCAGATCCACC
AAAGCAGCCAGCAACAGGGCTGCCTCAGCCACCAGCAGAGACTTCGCAACAGGCCACTCTCCCGTGGCTA
CCCACCCTTTCGTAAACAACCTCAAGGCCACAAGATGTCACAGATGAGGCCAATGCCCTTTTTTGACATG
GAAGACCGGGATTCCCTTCCGGAGGGTCATTCCTGCCCGCATGCCAAGCAACCCCGTCGGGGCTGGGGTA
GTTTGTGTAAGCCAGTCCGTCTGGCCTCCAATGTGGGACTGTGGGGTCGCCAGGGTGGAATCCTTGCCAG
CCTCCCATTACCCTCCCTCTACCTGTCACCAGAGCTGCGCCGCTTGCCTAAGCGAGTAGAGGCCAAATCA
GAACTGAGGCTACAGGGCTTCGGTCCTCATTATTCACAGTCCCGGATTTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68557. Forward Primer - name:068557_F_cDNA_4933402P03Rik, sequence:TAGTCCAGGTTACCGAATGTCC; Reverse Primer - name:068557_N_SP6_cDNA_4933402P03Rik, sequence:CCAAATCCGGGACTGTGAATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12181 same embryo
 EMAGE:12185 same embryo
 EMAGE:12182 same embryo
 EMAGE:12186 same embryo
 EMAGE:12184 same embryo
 EurExpress:euxassay_011174 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS