EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12195

Gnb1 guanine nucleotide binding protein (G protein), beta 1 ( MGI:95781)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008601. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008601_01	euxassay_008601_02	euxassay_008601_03	euxassay_008601_04

euxassay_008601_05	euxassay_008601_06	euxassay_008601_07	euxassay_008601_08	euxassay_008601_09

euxassay_008601_10	euxassay_008601_11	euxassay_008601_12	euxassay_008601_13	euxassay_008601_14

euxassay_008601_15	euxassay_008601_16	euxassay_008601_17	euxassay_008601_18	euxassay_008601_19

euxassay_008601_20	euxassay_008601_21	euxassay_008601_22	euxassay_008601_23	euxassay_008601_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12195_wholemount_strong.wlz

12195_wholemount_moderate.wlz

12195_wholemount_weak.wlz

12195_wholemount_possible.wlz

12195_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12195_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6858
Entity Detected:	Gnb1, guanine nucleotide binding protein (G protein), beta 1 ( MGI:95781)
Sequence:	sense strand is shown >T6858 GCCGCGCCCATCCTGATGGCTGGAGGCGTCTGAGGGGCGGACGGCGACGGCGGCGGCGGCGGCGGCGGTG GCGGGCGCGGGCGGCGAGGGCAGCGGGGCTGGGACTGGAACAGCCGCCGCGCGGACCCGACCAGCCTCGC CGACTCCTGCCCGCAGGCCGCGAGCGCGACGACCAGGCGGCGGCGTGGGCGCCAGACCGGGCGAGGGCGA GATAGACTTACATGTGTGGGAAACCAGACCAGAAACACTTCTGAGTCAAGAGCTTGGATTCTGAAGTGTG GAAAGCACTGAGACGTGAAGATGAGTGAACTTGACCAGCTGCGGCAGGAGGCCGAGCAACTGAAGAACCA AATTAGAGATGCTCGTAAAGCGTGTGCCGATGCGACTCTTTCTCAGATCACAAACAATATTGATCCAGTG GGAAGAATCCAAATGCGGACCAGGAGAACACTGAGGGGGCATCTGGCAAAGATTTATGCCATGCACTGGG GCACAGACTCAAGGCTCCTTGTCAGCGCCTCTCAGGATGGAAAACTCATCATCTGGGACAGTTATACCAC AAACAAGGTTCATGCCATCCCTCTGCGCTCCTCTTGGGTCATGACCTGCGCATACGCTCCTTCTGGGAAT TATGTGGCCTGTGGTGGCCTGGATAACATCTGCTCCATTTACAACCTGAAAACTCGTGAAGGGAATGTGC GTGTGAGTCGTGAGCTGGCGGGACACACAGGTTATCTGTCCTGTTGCCGGTTCCTGGATGACAATCAGAT AGTTACCAGTTCTGGAGACACCACATGTGCCCTGTGGGACATCGAGACTGGCCAGCAGACAACCACATTT ACTGGACACACTGGAGATGTCATGAGCCTGTCTCTTGCTCCTGACACCAGACTGTTTGTCTCTGGTGCTT GTGATGCTTCAGCCAAGCTCTGGGATGTCCGAGAAGGGATGTGCCGGCAGACCTTTACAGGACACGAGTC TGACATCAATGCCATATGTTTCTTTCCCAATGGCAATGCCTTTGCCACTGGCTCAGACGATGCCACATGC AGGCTGTTTGACCTCCGTGCAGACCAGGAGCTCATGACCTACTCCCATGACAACATTATCTGTGGTATCA CATCTGTTTCCTTCTCCAAGAGTGGCCGCCTCCTCCTTGCTGGGTATGATGACTTCAACTGTAATGTCTG GGATGCACTCAAAGCTGACAGAGCAGGTGTCTTAGCTGGACACGACAACCGAGTCAGCTGCTTGGGGGTG ACTGATGATGGCATGGCTGTGGCAACAGGGTCCTGGGACAGCTTCCTCAAGATCTGGAACTAAAGCCAGG AGCAGGTGGATGGATGCCATGGCGACTGGAAGACCATTCCAACCTTGAATCGTTACAGCGATAGCATATC CTATCCAACCATACTAACGTGGACACCCACACCGCCCCTCAGAACTTCAAAAGGGCAAGATTTTTTTTCC TTCACTTATTGCTGAAACCAAGAGCACAATTCCCATTAAGAGAAGGATTTTTGTGCTGTAAACTAAACAA ATTGTGCATTTTTTTTGGGGCCATTGTTTTTGGTTTATTTTGTTATTTTGTTTTGTTTTTTGTTTTTGTT TTTTTGGTTTCATTTTGTTTTGGGTTGGTCCAATTTTAAAAGGAAATACTACAATAAAAATGTTAACCAG AAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964965 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964965 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12191 same embryo
	EMAGE:12194 same embryo
	EMAGE:12193 same embryo
	EMAGE:12192 same embryo
	EMAGE:12196 same embryo
	EurExpress:euxassay_008601 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source