EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12221

Bace1 beta-site APP cleaving enzyme 1 ( MGI:1346542)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008281_01	euxassay_008281_02	euxassay_008281_03	euxassay_008281_04	euxassay_008281_05

euxassay_008281_06	euxassay_008281_07	euxassay_008281_08	euxassay_008281_09	euxassay_008281_10

euxassay_008281_11	euxassay_008281_12	euxassay_008281_13	euxassay_008281_14	euxassay_008281_15

euxassay_008281_16	euxassay_008281_17	euxassay_008281_18	euxassay_008281_19	euxassay_008281_20

euxassay_008281_21	euxassay_008281_22	euxassay_008281_23	euxassay_008281_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12221_wholemount_strong.wlz

12221_wholemount_moderate.wlz

12221_wholemount_weak.wlz

12221_wholemount_possible.wlz

12221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9212
Entity Detected:	Bace1, beta-site APP cleaving enzyme 1 ( MGI:1346542)
Sequence:	sense strand is shown >T9212 AGCCTGCCTAGGTGCTGGGAGCTGGGAGCTGGATTATGGTGGCCTGAGCAGCCGACGCAGCCGTAGGAGC CCGGAGTCCCTGTCGGTCCCCAAGCTGCAAAGCCCGCCTGGAAGACCCCGAAAGCTACGGGCTCGGATAG CCATGCCCGCCCCTCCCAGCCCCACAAGGGGCCCGATCCCCCCGCTGGCGGCCGGCGTCCAGATGTAGCT GGGTCCCCTGGATCGCCATCGTCGTCTCCTCTCGTGCGCTACGGATTTCTCCTGCCCACTCTCCGCCGCC TGGACCGGGAACTGAGCGAGGGGCCTGCAGACTCTGCAGTCCTGATGCCGCCGAGGCCGCTCTCCTGAGA GAAGCCACCACCACCCAGACTTAGGGGCAGGCAAGAGGGACAGTCACCAACCGGACCACAAGGCCCGGGC TCACTATGGCCCCAGCGCTGCACTGGCTCCTGCTATGGGTGGGCTCGGGAATGCTGCCTGCCCAGGGAAC CCATCTCGGCATCCGGCTGCCCCTTCGCAGCGGCCTGGCAGGGCCACCCCTGGGCCTGAGGCTGCCCCGG GAGACCGACGAGGAATCGGAGGAGCCTGGCCGGAGAGGCAGCTTTGTGGAGATGGTGGACAACCTGAGGG GAAAGTCCGGCCAGGGCTACTATGTGGAGATGACCGTAGGCAGCCCCCCACAGACGCTCAACATCCTGGT GGACACGGGCAGTAGTAACTTTGCAGTGGGGGCTGCCCCACACCCTTTCCTGCATCGCTACTACCAGAGG CAGCTGTCCAGCACATATCGAGACCTCCGAAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAGG GGGAACTGGGCACCGACCTGGTGAGCATCCCTCATGGCCCCAACGTCACTGTGCGTGCCAACATTGCTGC CATCACTGAATCGGACAAGTTCTTCATCAATGGTTCCAACTGGGAGGGCATCCTAGGGCTGGCCTATGCT GAGATTGCCAGGCCCGACGACTCTTTGGAGCCCTTCTTTGACTCCCTGGTGAAGCAGACCCACATTCCCA ACATCTTTTCCCTGCAGCTCTGTGGCGCTGGCTTCCCCCTCAACCAGACCGAGGCACTGGCCTCGGTGGG AGGGAGCATGATCATTGGTGGTATCGACCACTCGCTATACACGGGCAGTCTCTGGTACACACCCATCCGG CGGGAGTGGTATTATGAAGTGATCATTGTACGTGTGGAAATCAATGGTCAAGATCTCAAGATGGACTGCA AGGAGTACAACTACGACAAGAGCATTGTGGACAGTGGGACCACCAACCTTCGCTTGCCCAAGAAAGTATT TGAAGCTGCCGTCAAGTCCATCAAGGCAGCCTCCTCGACGGAGAAGTTCCCGGATGGCTTTTGGCTAGGG GAGCAGCTGGTGTGCTGGCAAGCAGGCACGACCCCTTGGAACATTTTCCCAGTCATTTCACTTTACCTCA TGGGTGAAGTCACCAATCAGTCCTTCCGCATCACCATCCTTCCTCAGCAATACCTACGGCCGGTGGAGGA CGTGGCCACGTCCCAAGACGACTGTTACAAGTTCGCTGTCTCACAGTCATCCACGGGCACTGTTATGGGA GCCGTCATCATGGAAGGTTTCTATGTCGTCTTCGATCGAGCCCGAAAGCGAATTGGCTTTGCTGTCAGCG CTTGCCATGTGCACGATGAGTTCAGGACGGCGGCAGTGGAAGGTCCGTTTGTTACGGCAGACATGGAAGA CTGTGGCTACAACATTCCCCAGACAGATGAGTCAACACTTATGACCATAGCCTATGTCATGGCGGCCATC TGCGCCCTCTTCATGTTGCCACTCTGCCTCATGGTATGTCAGTGGCGCTGCCTGCGTTGCCTGCGCCACC AGCACGATGACTTTGCTGATGACATCTCCCTGCTCAAGTAAGGAGGCCCGTGGGCAGATGATGGAGACGC CCCTGGACCACATCTGGGTGGTTCCCTTTGGTCACATGAGTTGGAGCTATGGATGGTACCTGTGGCCAGA GCACCTCAGGACCCTCACCAACCTGCCAATGCTTCTGGCGTGACAGAACAGAGAAATCAGGCAAGCTGGA TTACAGGGCTTGCACCTGTAGGACACAGGAGAGGGAAGGAAGCAGCGTTCTGGTGGCAGGAATATCCTTA GACACCACAAACTTGAGTTGGAAATTTTGCTGCTTGAAGCTTCAGCCCTGACCCTCTGCCCAGCATCCTT TAGAGTCTCCAACCTAAAGTATTCTTTATGTCCTTCCAGAAGTACTGGCGTCATACTCAGGCTACCCGGC ATGTGTCCCTGTGGTACCCTGGCAGAGAAAGGGCCAATCTCATTCCCTGCTGGCCAAAGTCAGCAGAAGA AAGTGAAGTTTGCCAGTTGCTTTAGTGATAGGGACTGCAGACTCAAGCCTACACTGGTACAAAGACTGCG TCTTGAGATAAACAAGAACCTATGCGATGCGAATGTTTATACTCCTGGGGGCAGTCAAGATGAGGAGACA GGATAGGATAGAGACAGGAAGGAGATGGTAGCAAAACTGGGAAAGGCAGAACTCTGATCACTTTCTAGTT CCAAGTTTAGACTCATCTCCAAGACAGAAGCCCATCTGGACTAAGAGGTATCATTCCCCAATGTGCCTGT GGTTGTAGTCTGAACTGAAATGAAATGGGGGAAAAAGGGCTTATTAGCCAAAGAGCTCTTTTTAACACTC TTAGAGGAACAGTGCTCATGAGAAAAGTCCCACTGGACAGATGAATTCCTATCTTGTTAATTCTGTCTCT CTCTGCTTCTTCAACATGCTAAGTGGCACCAAAATGACCCAACCCCAAGGTCTTAGGTGCCCTATGGGAC AACAGTTAGAATATTGTAGGGCTAGGGATGGTCTTCCCAGCATAGGTTCACTCCAACCAAGGTGCTAAAA GGAACAGACAGGAGAGTCCTCCTCTCTGATCCACAAAGGCAGAGCCCTCAAGATTCATCCAGCAGGGTTA GGGCTGATGCATTTGCCTCTGCCTGGATTTTGTTTTTATTTTCTTTCTTTTTGCCCAGTGGTACAAAACG ATAAGCTCTTTATGGAATACTGAGTGGGTTCATTCCTCTCTTGCCCTCTCCAATGGCCCCTCTATTTATC TGGCTAAGGAAACACCACGCATTGGCTAGTATTAAACAGCAACTGTAAGATAGAGGGCTTTCTGTTCTAT GTCATTGCCTTCAGTATCAAGGCTGCCTGGAGAAAGGATGGCAGCCTCAGGGCTTCCTTACTTTCTTCTC CTTTCCTGACAGAGCAGCCTTTCTGTCCTGCTCTCTGCTGCCCCTCCCAATATAATCCATGGGTACCCAG GCTGGTTCTTGGGCTAGGTTGTGGGGGCCACACTCACCTCTTCCCTGCCAGTTCTAACACGACAGACATG AAGCCAGTGTTAGTGGGAAGAGCTGGGTTTTCCCAGGATGACCACTGCATCCTCTCCTGGTACGCTCTAC ACTGCTTTCAGGCTGGGGACCTGCCAAGTGTGGGACAGTTGATGAGGAAGAGACATTAGCAGGGCCTCTG GAGTTGCTGGCCCAGCCAGCTGCCCACAAGCCATAAACCAATAAAATAAGAATCCTGCGTCACAGTTTCC AGCTGGGTCCTCTTCCTTGCCCTCGCACTGGTGCTGCTCTGGCTGAGTAGGAATACACCCACAGACTGCC AGGAAGATGGAGACTGTCCGCTTCCGGCTCAGAACTACAGTGTAATTAAGCTTCCAGGATCACTACCATG AAAACGCCGCATTCTGCTTTATCATTTCTACCCATGTTGGGAAAAACTGGCTTTTTCCCCATTTCTTTAC AGGGCAAAAAAAAAAAAAAAAAAAGGGAGAGAGAGAGAGAACTCAACCTAGTTGTTATTTACCCTAGTAA CTGGTGTTCTATTTTTTTTTAAAGGGGGAAAATTTGCATTTATTTTTCTTTTGATGGTTAACTCCTTTGT ATCATAAAATTATGAACTCTGATATGTAAAACAGAAAAAAATCTTGACAACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831622 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831622 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12226 same embryo
	EMAGE:12224 same embryo
	EMAGE:12225 same embryo
	EMAGE:12223 same embryo
	EMAGE:12222 same embryo
	EurExpress:euxassay_008281 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source