EMAGE mouse gene expression spatial database

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EMAGE:12222

Ttyh3 tweety homolog 3 (Drosophila) ( MGI:1925589)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008337_01	euxassay_008337_02	euxassay_008337_03	euxassay_008337_04	euxassay_008337_05

euxassay_008337_06	euxassay_008337_07	euxassay_008337_08	euxassay_008337_09	euxassay_008337_10

euxassay_008337_11	euxassay_008337_12	euxassay_008337_13	euxassay_008337_14	euxassay_008337_15

euxassay_008337_16	euxassay_008337_17	euxassay_008337_18	euxassay_008337_19	euxassay_008337_20

euxassay_008337_21	euxassay_008337_22	euxassay_008337_23	euxassay_008337_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12222_wholemount_strong.wlz

12222_wholemount_moderate.wlz

12222_wholemount_weak.wlz

12222_wholemount_possible.wlz

12222_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12222_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9214
Entity Detected:	Ttyh3, tweety homolog 3 (Drosophila) ( MGI:1925589)
Sequence:	sense strand is shown >T9214 GGTGAACAAAGAGGCGGCGGGCGCGGGCGGCCGAGCGGAGCCGAGCGCAGCCGAGCCGGGCCGAGCCGGG CCGAGCCGGGCCGGGCCAGGGCCCCTGGAAGCGGGCACGATGAGGGCAGCTGAGCAGGGGTCGGCGGGTC CCTGAGCCCCGTGCATCCGGCAGCGAGGGAAGCCCGGCGCAGCGCCCGTGGCCGCCCGCCCGCCATGGCC GGGGTCAGCTACGCGGCGCCCTGGTGGGTGAGCCTCCTGCACCGGCTGCCGCACTTCGACCTGCGGTGGG AGGCCACCAGCAGCCAATTCCGGCCCGAGGACGCCGACTACCAGCAGGCGTTGCTGCTGCTGGGGGCCAC CGCCCTGGCCTGCCTGGCCCTAGATCTCCTCTTCCTACTCTTCTATTCCTTCTGGCTGTGCTGCCGGAGG AGAAAGACAGACGAGCACCTTGATGCAGACTGCTGCTGCACTGCCTGGTGCGTCATCATCACCACACTGG TCTGCAGCGCGGGCATTGCAGTGGGATTCTACGGCAACGGCGAGACCAGCGATGGCATTCATCGAGCCAC CTACTCACTACGCCATGCCAACCGCACAGTGGCAGGGGTCCAGGACCGCGTGTGGGACACGGCTGCCGCC CTGAACCGCACAGCGGAGCCCAACCTGCAGAGCCTGGAGAGGCAGCTGGCTGGGCGGCAGGAGCCACTGA GGGCTGTGCAGCGGCTGCAGACCCTGCTGGGGACCCTGCTGGGCTACACCGCTGCCATCCCCTTTTGGAG GAACCCCGGAGTATCGCTGGAGGTGCTAGCTGAACAGGTGGACCTCTATGACTGGTACAGGTGGCTGGGG TACCTGGGCCTGCTCTTGCTTGATGTTATCATCTGCCTCCTGGTGCTGGTGGGCCTCATCCGAAGCTCCA AGGGTATCTTGGTTGGGGTCTGCTTGCTGGGTGTCTTGGCCCTGGTCATCAGCTGGGGTGCGCTCGGCTT GGAGCTGGCTGTGTCTGTGGGCTCCAGCGACTTCTGCGTAGACCCTGACACCTTTGTGACCAAGATGGTG GAGGAGCACTCAGTACTGAGTGGGGACATTTTGCAATACTACTTGGCTTGCTCACCCCGTGCCACCAACC CCTTCCAGCAGAAGCTGTCGGGCAGCCACAAGGCTCTGGTGGAAATGCAAGATGTTGTCGCGGAGCTGCT GAGGAACGTCCCCCGGGAGCACCCCGCCACCAAGGATCCCTTGCTCCGAGTCCAGGAGGTGCTAAATGGA ACGGAAGTGAATCTCCAGCACCTCACCGCCCTGGTGGACTGCCGCAGCTTGCACCTGGACTACGTGCAGG CTCTGACAGGCTTCTGCTATGATGGTGTGGAGGGCCTCATCTACCTGGCCCTCTTCTCCTTTGTCACAGC CCTCATGTTCAGCTCCATCGTCTGCAGCATCCCACACACCTGGCAGCAGAAGAGAGGTCCAGATGACGAT GGGGAGGAAGAGACTGCGCCAGGGCCGCGGCAGGCACATGACAGCCTTTACCGAGTGCACATGCCCAGTT TGTACAGCTGCGGAAGCAGCTATGGCAGCGAAGCCAGCATCCCAGCCGCTGCCCACACCGTCAGCAATGC CCCAGTCACCGAGTACATGAGCCAGAATGCCAATTTCCAGAACCCCCGCTGTGAGAACACCCCCCTCATT GGGCGCGAGTCCCCACCGCCCTCATACACCTCCAGCATGAGAGCCAAATACCTCGCCACGAGCCAGCCTC GCCCCGACTCCAGCGGCAGCGGGCACTAGACCGCACACCGGCCAGCCATCTGCCCCCACGTGCCAATGCC CCACCCTTCCTATGCCAGCACCGCTGCTTCCGCCCTGCTGGACCTTCCACAAGCCACACCGGGCCAGCCC TAGGCACACCTGAAGGCCCCCCCGCTATACCCACCCTGCAGGAGGCATGGAGGTGCCCAGCATGAGCTCC GGGCCAGCCTGTGAAGTGAGGCTTCTGGGGTGCTCACCTGGACAGTCGCTGCCACCAGCAACCTGACCCT GCCTGCCTCCGTCTCCCCTGCCCTTCTGTCCTCTTGGGCTCCCTGAGCCCTGCTCTGTGCCGGGTGCTTC CACCCTGCCTGCGTTCCTTCTACAGGTGACCCTTCATGCTGCCCCTTGCACTCTGGGCGTCCCCCTCCCC ACAGCTACAGCGAGCCTTTCCTGGTACTGCCCTACCGCCACACTCCTGAGAATCCCATCTCCATTCACAG GCAGGGGACCCTGTGCCTGGCGCCCACCTGCTCATCACCGAACGGCCCCTTCATACTCTCTGGTCTTTGC AACGTCCGGCGCCTGCCTTCCCTTAGCCACCTGGCTTACTTGGCTCTTCCTGTTGGTTCTGAGCTGCCTC TAGGGTGGGGCTCTTCTTACACCTCCTCCCCACATGACTCTTCCCTCAGTGCCCAGCCAGCCCTCCCCAG ACAGCAGGTGCCAAGCTGAGCCCAGGTCACTCCCTGGCCTCTCAGTGCTGGCCACCTTCTTGGTGCCAAA CCCTCTTTCCCATTCATTCCTGAAGAGTGGCAGCTTTGTCTTCCCCTTTGTTTTTGCACTGACCACACTT GTCATCTCCAGGGCAGTCTGGGACATAGGCGACGCAGGACACTGCCCACTTTCCTCTCGGTTTCATTCTC ACCCAGGACAAGCGGCAGTGCCCTCTGGAGCCCAGCCTTGGGGGCTGGACCTGTGACGGCTGTGAATGTG CCCCCAGCCTCAGAAGGCCCCCCCCACCCCCCGCTGGGACTAACCACTAACCTCACTCTCAGCTGCCATG GGCACTCCTGGCTGACCCTGAGCCAGCAAGCGTGACCCTAAGTCTAGCCTGGAGCCAGGGCTAGAGTGGT CATTTCTTTGTGGGGTGCTGCCAGGGAGGGGCCAGACCCACAGGCTACTCAAAGGGCCTAGAGACCCCTC CCCAGGCAGGTGCTGCCCCAGGAGGAGCATGTCCTGGGGTCCGGGGACTGAAGTCCATGTGGCCTCAGCC CCCCACACCCAGAACACCGCTTGCCTAAGGTGCTTTTGGCTTTAGTGTGTGATGTTTGCTGTGCTTCTGG GCTGAATTAGCTTCCAAATCAGGACCTGGAGCCTCTACCCTGGCCCAGCCAGCCAGTGTGAGCTCTGGTC TGTGAGATGGGCAGCTACGGGCCAGTGGAGCAGCATGTGGTGGGAGGGGCAAGGCTGGGACCCAGTGGTT TACAGACCTGTGGCCCTCCTGGAGCAACCTGGCAGCTACGGATCCCAGAACCCCCTGGGCTTCAGCTCCC CCAGAGGGGAGAGGCTCCACGTTGCTTTCCTTCCCCAAAATCCCTTTCTTTGTGCTGGTGTCTGGGACCA AAAGGAGTGGGCAGAGGACTCGGAGGGCCTAGGGGTCCCAGTCGGGGCATCTGTAGCTCCTAAGCACGAC AACATCAGTGCAGGGGACCCTGGCCTTGACTCCAACTGGCCTGGCGCCAGGAACCTCCAGGGCCAGAGCA GCCCAGCTGCAGCCAGCCTGCCCACTATGGGTATGTTCCTGGCCTAAGGTCCGGAGGGAGGTTTGGGGTA TCCCTGCCTGGGTGCCTGGGTGTGCCCTGGGGCCTCTCAGAAGCACAAATGCTGCCCCCTGGCCGTGAGC AGGCCACAAGGTGAATGTATATAGCATGAGAGGCGGGCACTGCCCGGACGTGGCTGTGAACTTGTGCTGT CTCGGGAGTCCTGACCTTCTGTGCGTGAGTGCCCCCATCTGTGACGTTTCACTCACCGAGGCTGAAGAAA GGAAGCAGGGGAAATGAAAGCAGGGGTTTCTCGCCCTGACCCCTGCGGAGGAGACGGCTCCTACCACTGC GGTTGGCTTCATTTCGTTTTCCTGATTTCTGGGGTGCCACTTACCTACTCAATCCCAGTGGTCCACCCCC ACATCCCCAGGGAGTGAGCAGTCCAGTGCCAGCTGCCTGTGATTGGTCCCCAGTCCCTATTACCCAAGGG GACCCTACAGCTCTGGTGGGTAACAAGGAGGGCTAAGCCACCAAACCAGAGCCCGATCCCTTGCCGAGCC AGGAGGAGGGATCTGGCTGAGAAAACTGATAGGACTGGAGGCCCCCACCCCAACCAACACTCTCTGGTTT ATGTGAGTAGCAGAAGATCCCGGCCTGGAGCATCCTTCAAGCCCTTCTCCCTGTGCCCACCCCGCCCCCC CCCCCCCCCATATCACTATGCAATTCTTGACCCCAGCTCCAAAGCTTGCCCTACCCGGTCCCAGCTCTGT CCGGCCCAGAAGGTGGCTAGCTGGTGGGCCACAGGTGACCAGGGTCTCTTTGTTTTTCATCACAGCGGTG GTGTGCCGCACCCTTCCTCCCATATGTGATTTTGTGAGATTGCCTCCCAGTTACGGTCCCTCTGCCTGCA TCTGCCCCCAGTGGACTATGTCATCTGAATCGAGCCAGCCCCAAGTTCCCCTCCAGCCTCTGTAGGGCCA TGGCTGTGTGTTACTGTTGCTGTGCTTTCATTTTTTAAACTGGGTTTGGGGTTTGATTTTTATTTCTGTG GGGAACTTTATTTTTCTTGGCAAATAACTAAAGTTCTTGTCCATGTAATTTCTGTGGTCTCTATTCAGCT TGGGTTTCATGTTTTAAAATAAACAATTTTAAGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831835 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831835 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12226 same embryo
	EMAGE:12221 same embryo
	EMAGE:12224 same embryo
	EMAGE:12225 same embryo
	EMAGE:12223 same embryo
	EurExpress:euxassay_008337 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source