EMAGE mouse gene expression spatial database

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EMAGE:12223

St8sia2 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 ( MGI:106020)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008334. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008334_01	euxassay_008334_02	euxassay_008334_03	euxassay_008334_04

euxassay_008334_05	euxassay_008334_06	euxassay_008334_07	euxassay_008334_08	euxassay_008334_09

euxassay_008334_10	euxassay_008334_11	euxassay_008334_12	euxassay_008334_13	euxassay_008334_14

euxassay_008334_15	euxassay_008334_16	euxassay_008334_17	euxassay_008334_18	euxassay_008334_19

euxassay_008334_20	euxassay_008334_21	euxassay_008334_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12223_wholemount_strong.wlz

12223_wholemount_moderate.wlz

12223_wholemount_weak.wlz

12223_wholemount_possible.wlz

12223_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12223_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9222
Entity Detected:	St8sia2, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 ( MGI:106020)
Sequence:	sense strand is shown >T9222 GGGCTCGCCGCGCCAGAGCAACCCTTGTCTGTTGCTGCTGCTGTCGCCTTGGCCGCCGCCGGCCAGGACT CGCCCGGAGCGCAGGGTGTGTGCCCTGCTGCGCGTGGAGCGCGGAGGCTCCTTCAGCCCCAGCTCCTCGC CCCGGCCCGCGCGCGGGTCCCGGAGGGCACCGACCCCACCATGCAGCTGCAGTTCCGGAGCTGGATGCTG GCCGCGCTTACGCTGCTCGTGGTCTTCCTCATCTTCGCAGACATCTCAGAGATCGAAGAAGAAATCGGGA ATTCTGGAGGCAGAGGTACAATCAGATCAGCTGTGAACAGCTTACATAGCAAATCTAATAGAGCTGAAGT TGTAATAAATGGCTCTTCACCGCCAGCTGTCGCTGACAGAAGTAATGAAAGCCTTAAGCACAACATCCAG CCAGCCTCATCCAAATGGAGACACAACCAGACGCTCTCTCTGAGGATCAGGAAGCAAATTTTAAAGTTCT TGGATGCGGAGAAGGATATTTCTGTCCTTAAGGGGACCCTGAAGCCTGGAGACATTATTCATTATATCTT TGATCGAGACAGCACAATGAACGTGTCCCAGAACCTCTATGAACTCCTCCCCAGAACCTCACCTCTGAAG AATAAGCATTTCCAGACTTGTGCCATCGTGGGCAACTCGGGGGTCTTGCTGAACAGCGGCTGTGGCCAGG AGATTGACACACACAGCTTTGTCATAAGGTGCAACCTGGCTCCAGTTCAGGAGTATGCCCGGGATGTGGG CCTCAAGACAGACCTAGTGACCATGAACCCCTCTGTCATCCAGCGGGCCTTTGAGGACCTTGTGAACGCC ACGTGGCGGGAGAAGCTGCTGCAGCGGCTGCACGGCCTCAATGGGAGCATCCTGTGGATCCCTGCCTTCA TGGCCCGGGGTGGCAAGGAGCGTGTGGAGTGGGTCAATGCTCTCATCCTGAAGCACCATGTGAACGTGCG CACGGCTTACCCTTCCCTGCGCCTGCTGCACGCAGTCCGAGGATATTGGCTGACCAACAAAGTCCACATC AAAAGACCAACCACCGGCCTCTTGATGTACACCCTGGCCACACGCTTCTGCAATCAGATCTACCTTTATG GCTTCTGGCCCTTCCCACTGGATCAGAATCAGAACCCCGTCAAGTATCACTATTATGACAGCCTCAAGTA TGGCTACACTTCCCAGGCCAGCCCCCACACCATGCCCTTGGAATTCAAGGCCCTCAAGAGCCTACATGAA CAGGGGGCATTGAAACTGACTGTCGGCCAGTGTGATGGGGCTACGTAAGGAGGACAGCCCATGGGACTCA GTGGCTCACTTTCCTGCCAGCTACACCACAGGCAGATGGGAGTCGGGGTGGCACAAACAATAGAACAAGC AGGCTAGTGGTTTTCTTTGTTAAAGTGTAAAACAGTGACCAGAATATATATATCTATACCTGCATATATA TATTGACCTGAGTATTTATAACTGTGTGGTGTTCATCTAGCACTAGGCAGATAAGCCACAGGAAGAAGGT GTGGAGAACCCACCTGGAACAGATGGAGACTCAGTCCATCTTTGGGGGCAGAAGGACTTGACATGAAAAG AAGCCAGTCCCATGACTTTGGATGACAACCCACTTCCTGGGCTGGATGGATCTTTGGGCTCATGGATGAA TGGAGAGCCACCTGTTGTCAGCTTCCCCGAACCTCCAAGTGAAGTGGATTCTGGGTAGCCCAGACCCAGA GATGACAGCTTGGACCAGTCTAAAGAGGTGCAGTTCATGGAGGAGAGACTTGGAAAATTCCATTGGGCAA AGAGGCACAAGGAGAGCAAGCAGCACTGATCACAACGGAGCTGAGGGCTTTGTTCATGAACTTGAGAGTG CAGCTGCCCAAAGGACAAGTATGCATGGCACCTGCGTCCCAGACAAGCCTTCACAGCTATGGAGAAAGGC TGGAGGAAACTCAGTGACAGCCCCATATCCTGGTTAGACCAGTACCACCCGGGACTTAGTAGGGCAGAAG AGCACATGGCCCTAAAGAGGTAGATTGTTGCAAATCGTGGTTTTAGAAACTTGGCATCTTCTAGGAGTTT GCTCCACCTCAGAAAAGGACAAAAGGGTGTACAAAATAGGATCATTTTGTTGGGTGCAGCCCACGGAGGC AGAAGTAACCCTAGGACTTCAACTTCCAGGTTAGCTTTTATCCCATCCTCTTAGAATGTCACATGAAGGA AACTCTACTGTCCCATCTACTGCCAGAAAGACACAGCAAGTGCTTGAAAGAGCACAGTCACAATAGGTGT CATGTTGTTCAAAACATATGCAGTTTTCTAGGCACCTCACAGAGCCCTAGAACTGGACACCTGGAAGACT GTGACAAATCTAACCCTGGGTCTCTGCAGTCACTCCCTGTTTGCTTTCTGCATCCTGCACTCTGGAGTCG CCTGCCGTGTCAATGTCACCTTGTCACTTTCCCATCTTGACAGTCATACCTGCCCACACCAAGCTCTTCT GGGAGAGGTTCCAGTGTCTACCCTACTACATTCCTGCCCACCCTCACCCCCCTACCCCATCAACCATTGC AGAGTGTTTGCAAAAGCCTTCCCCACTCTGTTAGGCCTCCCACTTAAGATTTGTTTTTCTTGTAAAAAAG CCCAAAGCCAAACACAGTTCCAAACACTGGACATAATCTCCGGGGGAAATCTGGGCTCCATGCCAACAAC TTAGGGGTTGGACTTACAGGTCACACACAGCAAAGCATTAGCCAGCTTGATGTGTTAGATGCAGTTCCCT CGCCTGATAAATAATGGTGTTTTAAAATATATTTGAAAGATAAATGTTTAAAAAAAAAAACACTATTATC TTGAAGATGAAGAGAGGTTTTTTTTTTAAGTAATCAAGATCTGCTCCCAAGCATATGAGGCCTGACGTAA TTAATCAACAGCCATTCAGAACACGATGCTTGCTCTAAATTAATGGCTGATGGGAAGTGAGCAGAGACCA TGGAGGGATACCCTAGAACTATTAGCCCTGCGTACATTCTCTCCAGCCCCAAACAGGCCAAGGACTGTAC ATCTCTGTTCCCCTCTTTGTTCTTGTTACAGTATGAAGCTTGAGATTTGGGATTTGCATCATAAAATGAA TATTTAAAAAAGTAACAGCCATAACAGCCTCCCTGGGAGCTGAGTGGCTCAGCCTCCTCATGAGCAGTGG AGATAATTAGTATCATATTTTACATGTCTCCAGAGCCTTTCATCTGAAGGTTCTCAAAGGCTCTAGAAGA CATTTAATTTCTAAAGTTTGCCGTGCCCTGGAGGAATAGGTACGATCACCAACAGGGCCTCGAATTAGTA AAACCCTTGGATGTCAGAACATCCCATGTGAATCATGAAGAAGGCCCAGAGGTCAGGTTCTGGAGGTGAC CCTGAGCCCCACCCAGCATCCTCTCTGTGAAGAGGAGTTAGAGGGGTAATTGTCTAACTAGACTTATGCA GGCTGTAACCTGATGGGAAAGGGCAGAGGTCCCAGGACTACCCATGTATGCCCTTTCCCTAACTCAGCAG CCCCTGTGCTGTTAGAATGGTCTGGACATGTAGCTGCAGGGTCTCTGTGGACCTTGGCTTCCCCTCTTGC CATTCTTCTCCAGTCAGCAGCCAAGTGTCCTTTACTGCCTAGTGAGAGCGCACTTGGAGCTGGGCTCCAC TGAAGTCTTTTAACATGGGCTGGTTGACTTTGGCTGGCCCCAGGGAGACACAGCCTGCACTGTTAACCTC ATAGCTACCACACAACATCACATCAGCCTCAGCCAGTCCCCTCAACTGTTGGCTTTCAGTCACCTCCTCA GTCTGCCTGCCTAAGCCTGCCAGACATCCATTTACTCTAAGCCCCCAGTGACACCACCCTCAGCCTCTGC CATCACCAAAAAAGAAAAAGAAAAAAAAGGGGGGGGGGACCATTTGGCATGAGACTTTATCTCATAATCT CTTGGCCTCTCCCTCCCACTTAACCTTCATTCTTTGACAAATGAGGTACCTCCTGTAAATGGGGAGTCTT TCCTCTCATGATCTCAGACTTTTCCCAGCCCCCCTTGCATTCACCCTGATTCCTATAGCCTCTCCACTCA ACTCCTCCACTACCAGACCTTGGCAGGGTCTGCAGTCCCTACTGCCACCAGTTCCCTGTACCACACCTTA ATGTCCCCTCTTACCAGCATCTCAGGCCCCTCCGTGTGCTAAATGCAAAGCCCTACAGCAAATTCCCCTG GGCAAGACACTAGGTTCTTAAGATTTTTATCCCTCCCATCCCAACCTCCATGACCATTTATTTATATATT TATTTATGTATTTATGAGGTATTTATTGGCATTCCTACCTTGAGCTGAAAAGAGCCAAGGAAGATCTGCC CATTTCCCCTCTCTTCTCCCTGTACCACAAAGTAGCCATTCCCTCCAACAAGGTCCTCCCCAAAGGCTTA TAAGCTGCGCATCCACAGTTCCATGCCTTCCTTCAGACTCAACTACTACAACCCAAAGAATACCCTTCAC TCCCCTTAGCTGTTTAGCTCCACTGGAAGCTCCCAGTTCCAGGATGATACACTGCTTTTCCAGCATACTC TATCTGCTCTGACATCTTGAACTCAAGCTCATCTGAAGCTAAGTAGACCATCTCTCTTCCCTGCATGATC CCTTATTTTCTGGTTGGAACACCAATGATTCTTTCAACACATTCTGGGACCCTAAGATGATCACTGACTC TTCCATGTATCCCCTCATTACCCTTTCCAACTAGTCCCAACCTCACCTCTCCTTGTCCCTTGGACTCCTT AGTAGTGGTCTCCTCTCCCAGACCTCCCTGAGCATGCACGATGGTATTCTCAGCTCTTCACGAACTCAGC TAAGATCTCCTGACCCACAGAAATGTGCCCATCTTTATCCTGACATTACACTCAATCAGGTCCCTTCATA GCCTGCACTGGCCAATCCAGAAGAGTTGTCAGTATTGGGGAAAGCACCACGTTTCCTGGCCCTGTGCCTT TGGCCTTGCAGTTCGCATCTTCTGGATCATTCTTGAGTTTCTTCCAAGGCCCCTGCAGAGACCACATCTG CCATGAAGCCCTCCCCGATCCATCAACGCCGGGAACTCTCCCCCCACAGAACCACCACAGCACTTTATTT CCCCCTTTCTCCCGGCACTTACCATACTCTGCCTTGTATTATAGCTCCTTGTGCACTTGTCCTATGTCTG TTTGTAGACTGTAACCCTGCCAAGGGCAGGCACTGTTTTATTCATCTGTATCCATCACAGCATTAGGCGT GGTGCATAGCACACAACAGTTGCTCAATAAATGTTTGTTGGATTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833487 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833487 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12226 same embryo
	EMAGE:12221 same embryo
	EMAGE:12224 same embryo
	EMAGE:12225 same embryo
	EMAGE:12222 same embryo
	EurExpress:euxassay_008334 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source