EMAGE mouse gene expression spatial database

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EMAGE:12224

Gmfb glia maturation factor, beta ( MGI:1927133)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008311_01	euxassay_008311_02	euxassay_008311_03	euxassay_008311_04	euxassay_008311_05

euxassay_008311_06	euxassay_008311_07	euxassay_008311_08	euxassay_008311_09	euxassay_008311_10

euxassay_008311_11	euxassay_008311_12	euxassay_008311_13	euxassay_008311_14	euxassay_008311_15

euxassay_008311_16	euxassay_008311_17	euxassay_008311_18	euxassay_008311_19	euxassay_008311_20

euxassay_008311_21	euxassay_008311_22	euxassay_008311_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12224_wholemount_strong.wlz

12224_wholemount_moderate.wlz

12224_wholemount_weak.wlz

12224_wholemount_possible.wlz

12224_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12224_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9211
Entity Detected:	Gmfb, glia maturation factor, beta ( MGI:1927133)
Sequence:	sense strand is shown >T9211 GGGAGTTGGCTGCCGAGCCATTCTTAAAGGGGCCCAAGAGATCGCACGGCAACGGGCCGCTGACGACCGG AAGGAAAATGAGTGAGTCTTTGGTGGTTTGTGATGTTGCTGAAGATTTAGTGGAAAAGCTGAGAAAGTTT CGTTTTCGAAAAGAAACCCACAATGCTGCTATTATAATGAAGATTGACAAGGATGAACGCCTGGTGGTGC TGGATGAGGAGCTCGAGGGTGTCTCTCCAGATGAACTTAAAGACGAACTACCCGAGCGGCAGCCTCGAAC CTTCATTGTGTATAGTTATAAATACCAACATGATGATGGCAGAGTCTCGTACCCGCTCTGCTTTATTTTC TCCAGTCCTGTCGGGTGTAAGCCTGAGCAGCAGATGATGTACGCTGGGAGTAAGAACAAGCTAGTCCAGA CTGCCGAGCTAACCAAGGTATTTGAAATAAGAAACACTGAAGATCTAACTGAAGAATGGTTACGTGAGAA ACTTGGATTTTTCCACTAATGTGAACTTCTGTGTTTCTAAAGTATTTATGTATTAACCTGACCATACTGG AACCAGACATAAATAGTTATTTATGCCTAAAAATGCACTGTTACTTACAGTTTGTTTCCTGCAGTAAAGA AAAATTCTTCATTTGTGCAGTTTGAACAAAGGGGAAATCATCTTCATAGTAATGAGACGTTGTGAAGTGT TCCTGCATCTGTCATTGTTAGGGGGACTACTTTTCTCCAGGGATGCTTTGCACCCTTTTGACTAATTTCT ATAATTTATGATTCAGAATTTGTAACTGTTTGCAGACACTATAGGAAGGTGGGTATTAGACTGTGATAGA CAACAGTTCCTCCTCTGACCACTCCTGGGTACTGGCAGTTTATAAGTGCAAGTAGCATTTTATCACCTGT CACATCATTGCGGTTCATTTGGGACTGAAGCGTAAGTGACCGATTCTGAGCCTTGAGTAGAGATGTAGTG GGACCTCTGTTGAACTCTCCGCCAGTTTTCCTCCTCTCTTGGGTTCCTGTATCTTTGCAATCAAGTGTAG AATCTTCATCAATCACTCCTCCTTAAGGTCCGGAAGTTGATTTTTATGGTAGAGTTAGGATTGTTAGGTT TTCTTCTTATGGAGAATAGCATCTGAATCTTAGAAACTGGGAGCTCTATAATCAAGGGCTTCGTTCTTGG TTATGTCACTGGCAGGGAGCTTAGACTGTAGCTTGGTAACACTGCAAAGCACCTCCTAATATGTTAATAC TGTGCCTTTGATTGGTCAGCTTTACCATTAGCTTAAGACTGCTACACTGCCAGTGTTCTAGATTTGGTGA AATTAATACTTTTTTAAAGGGTTCAAATAAAACAATTTTCTAATATATATAATTAAAATTTGTAATAAAC CAACTTGTTACTTTTAAAATTCCAACTATCTGCCCAAAGTTATGATAGTAGGTGAGAGTTATAATTTGGG GTATATTTGCTATTAGTTTTGTGCCATATGTAAGCATTATTTAAACATTGGCCACAATTAATAACAACAC ATCAATAGATGTCATACCTTTTATCCTAAATGTCAGAAGAAATTCTAATATGAACTAAGCCAGTCACAGT TTGATGACACCATCATCAGGAAGCAAGATTTCTAATCTACTTGGAATTCTTAAGTTTCACTTAAGAAAAC AATGTAAGAAATGCTGAATAGTAATTTGACTTAAAGAGACAAGTTAAGAGACTGTAGAGACGACCAAATT CTTATATTAAGAAGGTAGTGATTTTGATTGGGCATTGGTGGCACATAACATTTAATCCTGGCATTTGGGA GGCAGAGACAGGCAGATCTCTGAGTTCAAGACCAGCCTGGTCTGCAGAAGCAAGTTCCAGGGTAACCAAG GCTAAACAGATGAACACCCTGCTCCCCTAAAAAGAAAAAAATAGCAAAACATCTTTATGTTGGCATGTGT TCATTGCATAGTATTTGTCTCATTTAGGGCTTTGTGCCGGTTTTACTGGAGGGAAGATAGCTGGAACTGT CACAGTAGAGTGGAGTGTGTTGTATTCCCAACTGTGTGCTCACTAGCCTGTATGTCAGGAAGCAATTGCA AGTGCTAGAGGAGGTGATCATATTGGTTCATGAATGGAAAAGTCCCTTTATTTCAGTGTCCAGAGAGCAA GAAAACTTCATCTAAGAAGTTTAGGACTTCATTATACAGGTATTTTGGTATGAGCTAGAGAATAACTTGC TTATAATCCCACAGGAATGCAGATGAGACCCAAGTAAAGGGGGACTTTGATGTCTCTGACTGATGCACAT AAGGGTTCGTTTCACCTGGTTATAGAAGCACGCTTTCAGAAGCAGTGCTACAAGTCTACGTTTTAAATTC AGTTCTTTATTTTGGCTTGCATATTTAAATCAGTTTTTGAAATTTCCAAGTCTTTCATAGAGTGAATGGC CCTGACCTGCCACACACAGGACTCTTAGTCTCTAGTTCAAGCAGCAACACCAGTGGTGAGCAGCTTTCCT TTTCAAGCTTGTCTGACTCTTACCTGTAGATGGGTCGTTTGTAAGGTAGCACACTGAGTTGGGGCATTAA TAGAACATGGTTATTCTGTCTGTCCTTAGACAAGTTTGTAAGTCCTGTGCCCTGCAGTGTTCATGAAATA TTTATGTAAGTTTCCCTTTAGCTAAAATTTACAGTATTTTTCTTATTCTTTTTCTGCTGTTCCGTAAAGA GTATGTCCAGATTTCATAATTCCAGGTTTTTTACACTCCATGTCTTTCAGCAACAACCATTCCCACTCAG CCTTAATCTTAATCTTTCCTTTTTGGAAGCCACTCTTCCCGGGAACCTTTTAAGCAAGCATTGCTTTGGA AATTTCTGATCTTACTAAGTTGTGCATAGCATCTAATGTCAGAACCGACTAGGAATCACAATGTATATGT CATGTGGTTTTGGTGACAACATGAGTGACACATGAAGAGGGGTATTCTCAGGTTACCGTGCTGCAATTCC AAAAGGTCTGAGTTTTAAACAGTGATTTGTTCTGGTATTGACACAATTATGTTAGGAAAAAAGCTGGTTG ACTGTTTTGTTTAATTGACTTCTAAACTATGTTTGAATTGTCTGAGGAGTTTACTGGATGCTTAGCGCAG TTAAACATTCTCGTCCTGTGAGAGAAACATTCTCTTGTAGGAGAGGTGTTGCAGATTGTGATTATAAAAT AATTTGTAGCAAAAATATGTGTTAGCATTTTCCCTCCCTTTTTGTCTCCTATTTTCAGGAGACAAACTTA GCCATAAACTGAATCCTGGTCTGACACTTCCACAAATGGTTTCCAGTTAGAGGAGTTTATTGCCAAATTA AACTTGGCTCTTTTCCCACTTAGATAGAAACTAAGAAAGCTGCACATAAGAAATGTTTGGACACTTGTAA AACTCAAGTGACTACATTAATCTGTATGTAAACTAGTGAGCAGACACATCCATAAGAATTGAAATGCCAA TATTTGGGAAGTAGAGAGTAACTTATATCCTATCAGATCTATTGAGTGTTAATATGGTAGCACTCTGTAT TTGTTCTGTAGACTGATAATACTGGAATCCATAAAGTTTGAGCCTTTACAACTTATGTAAAGAAATGTTG CAAACATTTCTGGATGAGTCTTACTTTGTATCTCCAGAAGAATGTAGATGTCTCCTAGGCCTCCTCGTGC TGATGGTTCTGAGCTGAATATTTCTTGGTAGGCCATGTGACACTTCAGATCAGTGATCCTACTCCTGTAA TGTTAGGACAATTATATTACGCTTATGATAGAAACAGTTTCTTGCTTTTCGGAATATTATTACTTGAATA CCACTTGAGATCATTAGCATGCCAGGAAAAGTCCATTGATTTTGATGGTCCAATGTAATCTCATTCAGGA GGCTTGAAACATTGGTGGTTTAGTCTTGTGAATTTTAACAGTTCTCTGTCATTGTTTAAGAAAACCAACA ACTGGCACTACTTCTTAAGCTGTGGTTTCAGTCTCTGCTAGTTCATACCGCATGTTTATTTTGGAGTCTT TTGGTAAGCATGGTGCTTGTACTGGTTTAAATAAAATGCTAACATGAAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831501 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831501 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12226 same embryo
	EMAGE:12221 same embryo
	EMAGE:12225 same embryo
	EMAGE:12223 same embryo
	EMAGE:12222 same embryo
	EurExpress:euxassay_008311 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source