EMAGE mouse gene expression spatial database

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EMAGE:12225

Mtmr6 myotubularin related protein 6 ( MGI:2145637)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008326. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008326_01	euxassay_008326_02	euxassay_008326_03	euxassay_008326_04

euxassay_008326_05	euxassay_008326_06	euxassay_008326_07	euxassay_008326_08	euxassay_008326_09

euxassay_008326_10	euxassay_008326_11	euxassay_008326_12	euxassay_008326_13	euxassay_008326_14

euxassay_008326_15	euxassay_008326_16	euxassay_008326_17	euxassay_008326_18	euxassay_008326_19

euxassay_008326_20	euxassay_008326_21	euxassay_008326_22	euxassay_008326_23	euxassay_008326_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12225_wholemount_strong.wlz

12225_wholemount_moderate.wlz

12225_wholemount_weak.wlz

12225_wholemount_possible.wlz

12225_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12225_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 17 moderate expression: see section 16

pons mantle layer

moderate

regional

moderate expression: see section 07 17

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 10 11 18

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 16 17 18

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6608
Entity Detected:	Mtmr6, myotubularin related protein 6 ( MGI:2145637)
Sequence:	sense strand is shown >T6608 GTCACCGCCCCGTTTCTCTCCCGATGGTGCTCTCCTGAGGCCAGCTGCCTGCGGGCGTCGCCCCGGGATG GAGCACATCCGGACAACCAAGGTTGAACAAGTAAAGTTACTTGACCGGTTTAGTACCAACAACAAGTCAC TGACAGGAACACTGTATCTTACGGCCACACACCTATTATTTATTGACGCTCAGCAAAAAGAAACCTGGAT ACTACACCACCATATTGCCTCTGTGGAGAAACTTGCTCTGACCACTTCTGGATGCCCACTTGTGATTCAG TGCAAGAACTTCAGGATTGTGCATTTCATTGTTCCCAGGGAAAGAGATTGCCACGATATTTATAACTCTC TCCTGCAGCTATCAAAACAAGCAAAATATGAAGATCTCTATGCCTTCTCTTATAATCCCAAGCAAAATGA TACTGAACGACGGAATGGTTGGCAGCTTATTGACCTTGCTGCGGAGTATGAGAGGATGGGAGTGCCCAAT GCAAACTGGCAACTGTCGGATGCCAACCGAGAGTACAAGGTCTGTGAGACTTATCCCAGAGAGCTGTATG TTCCTCGGACAGCTAGCCGGCCGGTGATCGTGGGCAGTTCCAACTTCCGGAGCAAGGGCAGACTTCCGGT CCTATCCTACTGCCGGCAGGGCACTGAGGCTGCCATTTGTCGATGTAGTCAGCCATTGTCAGGCTTCAGT GCCAGGTGCCTAGAGGATGAGCATTTGCTTCAAGCCATTAGTAAAGCCAACCCAGGAAACCGCTACATGT ATGTTGTGGATACCAGGCCCAAGCTCAATGCAATAGCTAACAGAGCAGCTGGAAAAGGTTATGAAAATGA AGACAACTATTCTAATATTAGATTTCAGTTCGTTGGAATAGAAAATATTCATGTCATGAGATCCAGCCTT CAGAAATTACTGGAAGTGAATGGTAGCAAGGGGCTTTCTGTCAATGATTTCTACTCTGGTTTGGAAAGTT CAGGATGGCTTCGCCATATCAAGGCTGTTTTGGATGCTGCAATCTTCTTAGCCAAAGCAATAGTGGTTGA AAATGCAAGTGTGTTGGTCCACTGTTCCGATGGGTGGGACAGGACCTCCCAAGTGTGCTCCCTCGGCTCA CTGTTGTTGGATTCCTACTACAGGACAATGAAAGGATTCATGGTTTTAATAGAAAAAGATTGGATATCAT TTGGGCATAAATTTTCAGAGAGGTGTGGCCATTTGGATGGAGACCCGAGGGAGGTCTCACCAGTGTTCAC TCAGTTCTTGGAGTGTGTCTGGCACCTGACTCAGCAGTTCCCACAAGCCTTCGAGTTCAACGAAGCGTTT CTTCTTCAGATCCATGAGCACATTCATTCATGCCAATTTGGGAACTTCCTTGGCAACTGTCAGAAGGAGA GAGAGGAACTCAGGTTAAAGGAGAAAACTTACTCCCTGTGGCCTTTTCTTTTGGATGACAAAAAGAAGTA CTTAAATCCTCTCTACAGTTCCAAATCTCAGAGACTGACAGTCTTGGAGCCAAATACAGCCTCTTTCAAT TTTAAGTTTTGGAGAAACATGTACCACCAGTTTGATCGGACGCTCCATCCTCGGCAGTCTGTGCTGAGCA TAATTATGAATATGAATGAGCAAAGCAAGCAGTTAGAGGAAGACATTAAAGACCTCGAGGCTAAAATTAA GCAATGCAAAAATGGCATCCTAACGAAGGAGTTGCTGCATGCTGTCCACCCTGAATCACCTGCCCTGAAA ACCTCGCTGTGTCTCAAAGAGCCGAGTCTGCTTCCCGTGAAGGACACACTCCGAGCTATAGAGGGCAGCA GCCCAGCAGATAATCGCTACTGTGACTATGCCGAGGAATTTTCTAAGTCGGAGCCTACCGTGGTCAGCTT GGAGTACGGGGTGGCCAGAATGACTTGTTAGCCTCCTTCCGTGATCCCTGGATGACTGTAGTGTAAGAAA TGTATAGTTGTGAGGATGGTCTGTACACATGGGCAGGAGGGGTTGTTGAGTGACTTTGGGGCTTAGCAGT AAGTGAGAAAGTAGGTAGCTGAGAAAAGGATCATAGCAACTCTTGAGAGGAGTGAGTCAGCTAAGTGCCA TTTGTAACAAGAACATACTCATTTCTGTAGGAAGTGAGTGCTAGTGTGTGTATGTACTGGAAAGGGGTTA CACAGGACTGGTGAGCTGACAGTCTGTTGATTCAGATGTTAATTCCAGCCACGCTCAGTAGCTTTCCTCT AACTCTGAGAAAACAGACCTGACAACCTGAACCCTCAGTGACTGATGTGGCATGTGCTTTCAGTTCTGGA GCTGTGTGACTGTGGATTTTGCAGTCACAAAAATCCAAATATATAGTCAAAAATATAGTGACGTTTTAAC ATTAAAGTGCCATGAAAAGTACTTATGGGACAGCCTTAAAGTAGACTCTCAGTTTATTCTGTGGGTTTTA TAGCCGAGGACAGGATGTTTGATCTCTCCTTTAATCAGCCTTATTTCATATGAAGAACTAACTGCACTCT GATTACTTGGGGGCCGCACACTGCAGTGCTCCACCATGGAAAGTTGTAGACATACTCTGCTAGTTTGTAA TTAGCCAGCTCCACCCGTATTTAAAATCAGTGTTACCGAAGGCTTTGTTCTCGTTGTCATTTGTTCCAAG CTGCAGCTGACAGTTCATTATTCACAGCTTAGAGGACTCATTTCTTAGGCTAGTATAAGCACATTTGGCA TTTACTTTCGGCTTACTGCAGTCTATTAATTCTTACTCTGTGGTTGGCTTTAATCCTTTGATAACTGTAC ATTTAAAGTGGCAAAGCTCTAAAGCGTTCTGAGTACTAAATACATTTTTAACTTGACTGTAGCTGCCTAT AAACTTTCAGGTAAAGGAAAAAGGGAAGTGCGGGTAGTGGAGATTTTGCTTGGTTACGAATTCTAAAGCA ATCATTTTTGTGTGGAGCATATTTCCTTACTCTTAATATAGGAGGGGCAGAGAAGGGAGCACTGGGGGAG CCAGCTAATGTCCAACAGAAGCAGCTTTGACGCAGGAAAAGGTTTATTTTGCTTT
Notes:	The probe template was PCR amplified from IMAGE:3596732 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3596732 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12226 same embryo
	EMAGE:12221 same embryo
	EMAGE:12224 same embryo
	EMAGE:12223 same embryo
	EMAGE:12222 same embryo
	EurExpress:euxassay_008326 same experiment
	MGI:4826497 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source