EMAGE mouse gene expression spatial database

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EMAGE:12226

Csde1 cold shock domain containing E1, RNA binding ( MGI:92356)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007159. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007159_01	euxassay_007159_02	euxassay_007159_03	euxassay_007159_04

euxassay_007159_05	euxassay_007159_06	euxassay_007159_07	euxassay_007159_08	euxassay_007159_09

euxassay_007159_10	euxassay_007159_11	euxassay_007159_12	euxassay_007159_13	euxassay_007159_14

euxassay_007159_15	euxassay_007159_16	euxassay_007159_17	euxassay_007159_18	euxassay_007159_19

euxassay_007159_20	euxassay_007159_21	euxassay_007159_22	euxassay_007159_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12226_wholemount_strong.wlz

12226_wholemount_moderate.wlz

12226_wholemount_weak.wlz

12226_wholemount_possible.wlz

12226_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12226_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9223
Entity Detected:	Csde1, cold shock domain containing E1, RNA binding ( MGI:92356)
Sequence:	sense strand is shown >T9223 CTTATGGCGGCGCTGGAGAGGGGGCGCTGAGCTGTTGGGTATGAAGTGTAACAGAACAGACTTTACCACC TGAAACTGCTGCTTCAAGTTCAGATCAGGCAAGGAACAGACCCGCAACAACCAACAACACCAAAGAAGAG TACACAAGTTGAAGACACAACACTTGATCTGAAACAAGAAGTTTGTGCCTACTCAACAGCTTTGAAAGAG CACTTCCCAACGCTGCTAGTAGTCTTTGTTTTCTTCAGTGCTGTACTGTGAGATTGCCCGGTGCAGCAGC AGTTGTATTCTTTATTAGCTTGGTAGATCATTTCCTCTCGCTCTCCTTTTTTTTTTCTTTTTTTCTTTTT CTTTTTTTTTTCTTTTTTTAAATACTAGCAACTTTCATCCTTTGGAACTTGTGCTGAAAAAAGAAGAGTC AGCAAATACTACTGAAAGTGCAATATTTGATTATCACTGCGAGATGAGCTTTGATCCAAACCTTCTCCAC AACAATGGACACAATGGGTACCCCAATGGTACTTCAGCAGCACTTCGTGAAACTGGGGTTATTGAAAAAC TCTTGACCTCTTACGGATTCATTCAGTGTTCAGAACGGCAAGCTAGACTTTTCTTCCACTGTTCACAATA TAATGGCAACCTCCAAGACTTAAAAGTAGGAGATGATGTTGAATTTGAAGTATCATCTGACCGGAGGACT GGGAAACCTATTGCTATTAAATTGGTGAAGATAAAACCAGAAATACATCCTGAAGAACGAATGAACGGAC AAGTTGTGTGCGCTGTTCCTCACAACTTAGAGAGTAAATCTCCAGCTGCCCCGGGTCAGAGTCCAACAGG GAGTGTATGCTACGAACGTAATGGGGAAGTATTTTATCTGACTTACACCTCTGAAGATGTGGAAGGGAAT GTTCAGCTGGAAACTGGAGATAAAATTAACTTTGTAATTGATAACAATAAACACACTGGTGCTGTAAGTG CTCGTAATATTATGCTGTTGAAAAAGAAGCAGGCTCGCTGTCAAGGAGTAGTTTGTGCCATGAAGGAGGC GTTTGGCTTTATCGAAAGAGGTGATGTTGTAAAAGAGATATTCTTTCACTATAGTGAATTTAAAGGTGAC CTAGAAACCCTACAGCCTGGAGATGACGTGGAATTCACAATCAAGGACAGAAATGGTAAAGAAGTTGCAA CAGATGTCAGACTATTGCCTCAAGGAACAGTCATTTTTGAAGATATCAGCATTGAACATTTTGAAGGAAC TGTAACCAAAGTTATCCCAAAAGTACCCAGTAAAAACCAGAATGACCCATTGCCAGGACGAATCAAAGTT GACTTTGTGATTCCTAAAGAACTTCCCTTTGGAGACAAAGACACAAAATCCAAAGTGACCCTGCTGGAAG GTGACCATGTTAGGTTTAATATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGT TCTATCAAATACATTTCAGTTCACTAATGAAGCCAGAGAGATGGGTGTGATTGCTGCCATGAGAGATGGT TTTGGTTTCATCAAGTGTGTGGATCGTGATGCTCGTATGTTCTTCCACTTCAGTGAGATTCTAGATGGGA ACCAGCTCCACATTGCAGATGAAGTAGAGTTTACTGTGGTTCCTGATATGCTCTCTGCCCAAAGAAATCA TGCTATTAGGATTAAAAAACTTCCCAAGGGCACGGTTTCATTCCATTCCCATTCAGATCATCGTTTTCTG GGCACCGTAGAAAAAGAAGCCACTTTTTCCAATCCTAAAACTACAAGCCCAAATAAAGGCAAAGACAAGG AGGCAGAAGATGGCATTATAGCTTATGACGACTGTGGGGTGAAACTGACGATTGCTTTTCAAGCCAAGGA TGTGGAAGGATCTACTTCTCCTCAAATAGGAGATAAGGTTGAATTTAGTATTAGTGACAAACAGAGGCCT GGACAGCAGATTGCAACTTGCGTGAGACTTTTAGGTCGTAATTCTAACTCCAAACGTCTCTTGGGTTATG TGGCAACTCTGAAAGATAATTTTGGATTTATAGAAACAGCTAATCATGATAAGGAAATATTTTTCCACTA TAGTGAGTTCTCTGGTGATGTTGATAGCCTGGAACTGGGAGACATGGTTGAATACAGCTTGTCCAAAGGC AAAGGCAATAAAGTCAGTGCTGAGAAAGTAAACAAAGCCCACTCAGTGAATGGCATTACTGAGGAAGCTA ATCCCACCATCTACTCTGGTAAAGTCATTCGCCCTCTGAGAGGTGTTGATCCAACACAGATTGAGTACCA AGGAATGATTGAGATTGTGGAGGAGGGGGATATGAAAGGTGAAGTGTATCCTTTTGGCATAGTTGGGATG GCCAACAAAGGGGATTGCCTACAGAAAGGGGAGAGTGTCAAGTTCCAGTTGTGTGTACTTGGCCAAAATG CACAAACTATGGCCTACAACATCACACCCCTTCGTAGGGCTACTGTGGAGTGTGTGAAAGATCAGTTTGG CTTTATTAACTATGAAGTAGGAGATAGCAAGAAGCTCTTTTTCCATGTGAAAGAAGTTCAGGATGGCGTT GAGCTACAGGCAGGAGATGAGGTGGAATTCTCAGTGATCCTTAATCAGCGCACTGGGAAGTGCAGTGCCT GTAATGTCTGGCGAGTCTGTGAAGGCCCCAAAGCTGTTGCAGCTCCTCGACCTGACCGGTTGGTCAATCG CCTAAAGAACATCACCCTGGATGATGCCAGTGCTCCACGTCTAATGGTTCTTCGTCAGCCAAGGGGACCA GATAACTCAATGGGATTTGGTGCAGAAAGAAAGATCCGTCAAGCTGGTGTCATTGACTAACCACGTCCAC AAAGCACATCATTAATTCACTATGGTCGAGTTGGGGGGATTCTGGTGAAGGGTTCTGAATATCTTTCTCT TCATCCCTCCCAAAATCTGGAATACTTATTCTATTGAGCTATTACACCAGTTTTAACACCTTCCTCGTGT TATGTTTAAAGAAAAAAATAAATAAATTTAAGAAACCATTTTAAAATTATGCACAGTTGCAGCCTGGAAA ACTTAAAGGTGGCGCCTTATAGTGTCAATTTAGGAGCTTTATTTGGTGCATTTAACGCAACTGGTAATTG CAAATTCCACTTGGCCTGTGTAAGTGAACAATGCAGACTGTTACTTTGTTGGCCCTATGAAATTCTGCAC TCTAGTCAGTATATACTCTACCTTCATGACTTAACACTCAGCTGCATTCTTTTCCTATTTTTTCCCTGTT CATTATGCTTTAATTCTGAGGACCATATGAAGGTAGAATATATTACCTTTAAAAATTGCAAAAATTTATA TAGCAAACGATATTCTTAAGTTGATGGCCAAATGTTAAAATGTTATTTTCATATCATTTATAATCTTGTC ACAATCCACTTAACGAAGTTTGATTACATTTCAGTGAAAATTATCTTCCCGAGTAGTTTTTTTTTTTTTT TCTTCCTGGGATGGGGGGGGTAAATAACTTTACTACAATTAGTATGTGTGGTGCAGAATTTCATGCAAAT GAAGTGTGCCAGCAGTGTGATAATTTAAACATATTTAAACAAAAGACGAATGCACAAAATTGCTGCTGCT TAGATCACTGCAGCTTCTAGGACCCAGTTTCTTTTACTGATTTAAAAACAAAATGAACAAAAAAATAATA ATAATAAAAAAGTTGTGCCTGAAATGAATCCTGTTTTTTTAATAAATAGCCCCGTGGTTCCAGTGCCCTG TTCAATACAGGCACTTGATCCTTGGTGTAGCTTCTGTTCAACTTTGTATCACGGGAACGGATTGGCCTGA TTTCTCGGCCTTCATCTTGAATTGGCCCCAAACAGGGTCCCTGGCAAGTGGAGTGAAGGCTTTTTGTCTA ACAATGACAAGGGTCAGCTCAGGGGTTGTCGGGGAGGGCGCTTTCATCTTCCCCGTTGTCATTTGAGGTT TTGAACTCTGGGTAAAGAGGCCGTTTATCTTTGTAAACACAAAACATTTTTGCTTTCTCCGGTTTTATGT TAATGGCGAAAGAATGGAAGCGAATAAAGTTTTACTGATTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833505 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833505 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12221 same embryo
	EMAGE:12224 same embryo
	EMAGE:12225 same embryo
	EMAGE:12223 same embryo
	EMAGE:12222 same embryo
	EurExpress:euxassay_007159 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source