EMAGE mouse gene expression spatial database

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EMAGE:12227

Nol4 nucleolar protein 4 ( MGI:2441684)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008266. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008266_01	euxassay_008266_02	euxassay_008266_03	euxassay_008266_04

euxassay_008266_05	euxassay_008266_06	euxassay_008266_07	euxassay_008266_08	euxassay_008266_09

euxassay_008266_10	euxassay_008266_11	euxassay_008266_12	euxassay_008266_13	euxassay_008266_14

euxassay_008266_15	euxassay_008266_16	euxassay_008266_17	euxassay_008266_18	euxassay_008266_19

euxassay_008266_20	euxassay_008266_21	euxassay_008266_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12227_wholemount_strong.wlz

12227_wholemount_moderate.wlz

12227_wholemount_weak.wlz

12227_wholemount_possible.wlz

12227_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12227_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

neural retina

strong

regional

strong expression: see section 01 02 03 04 05 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9345
Entity Detected:	Nol4, nucleolar protein 4 ( MGI:2441684)
Sequence:	sense strand is shown >T9345 GGTTTCTGGAGGAGAAGGCTATGCTCTCTCTAAGGTGCATGATATTGGAGATATATGCATGAAAGGCTCT TCCAAGCACCATCTGGAGGACTCTCTCAGGATGGCGTAGGAGTTGATGAGAAGCTGTCTTTACGGCGAGT GGCTGTGGTTGAAGATTTCTTTGACATCATCTATTCCATGCATGTGGAGACAGGGCCGAATGGAGAGCAA ATCCGGAAACACGCTGGGCAGAAGAGAACTTACAAAGCAATTTCAGAGAGCTATGCCTTCCTACCAAGAG AAGCGGTGACACGGTTTCTAATGAGCTGTTCCGAGTGCCAGAAAAGAATGCACTTGAACCCAGATGGAAC GGATCACAAAGATAATGGAAAGCCTCCTACACTGGTGACCAGCATGATCGACTACAACATGCCAATCACA ATGGCGTACATGAAGCACATGAAACTGCAGCTTCTGAACTCACAGCAAGATGAGGATGAAAGCTCAATAG AAAGTGACGAATTTGACATGAGTGATTCAACACGAATGTCAGCCGTGAACTCTGACCTTAGCTCCAACCT GGAAGAAAGAATGCAAAGTCCCCAGACTGTCCATGGCCAGCAAGATGATGACTCTGCTGCAGAGAGCTCT AATGGCAATGAGACTCTGGGGCACAGCTCAGCTGCTTCAGGGGGAGCACATGGCAGGGAGCCCGAGGACT CCAGCAGTGATGGCAAAACCGGGCTGGAGCAGGAAGAGCAGCCACTGAACCTGAGTGACAGCCCCAGCTC TGCACAGCTCACTTCAGAGTTCAGGATAGATGACCAAGGCAGTGATGGGAAAAACAAGTATAAGAATCTT CTAATCTCTGACCTCAAGATGGAACGAGAAGCGAGAGAAAATGGAAGCAAGTCTCCAGCACACAGCTACT CCAGCTATGACTCTGGCAAAAATGAGAGTGTTGATCGAGGTGCAGAGGATCTGTCACTCAACAGGGGAGA TGAAGATGAAGATGAACATGATGAGCATGAGGATTCGGAGAAGGTGAACGAGACAGATGGTGTGGAAGCT GAGAGGCTGAAAGCTTTCAATATGTTTGTCAGGCTGTTTGTAGATGAAAACTTGGACCGAATGGTCCCAA TCTCTAAGCAGCCCAAAGAAAAGATCCAGGCTATCATTGACTCATGCAGGCGACAATTCCCTGAGTATCA AGAGCGTGCCAGAAAACGTATCCGTACTTACCTCAAGTCCTGCAGGCGGATGAAAAGAAGTGGTTTTGAG ATGTCTCGACCTATTCCCTCCCACCTTACTTCAGCAGTTGCAGAGAGTATCTTGGCTTCAGCCTGTGAAA GCGAGAGTAGAAATGCTGCCAAGAGGATGCGTCTGGATAAAGCACAGGATGAAGCTGCTCCTGCTGACAA ACAGTGCAAGCCGGAGGCAGCCCAGGCCACCTACTCAACAGCTACTGTTCCAGGCTCCCAGGAGGTATTG TACATCAATGGCAATGGGACCTACAGTTACCATAGTTACCGAGGGCTAGGAGGGCTGCTAAGCCTGGATG ACACTTCTAGTAGCGGACCCACAGATCTGAGCATGAAGAGGCAGCTGGCAACTGGCTCGGGATCGTCCAG CAGCTCAACCTCCAGACCTCAGCTCAGTCCGACGGAAATCAATGCCGTGAGACAGCTTGTCGCCGGTTAT CGAGAATCGGCTGCATTTTTATTGCGTTCCGCAGATGAACTGGAAAATCTCATTTTACAACAGAACTGAG GCAAACGATGTCTGTACTCACTGGGTTCTAAATTTGCAGTTTCCACTAATGACACTTGATTTTCCAGCAG AGATACTCCTAGGCTTGCTGCACAACCTTTTGAGGGAAGCATTTCCATCATGCCAGGCTGTCCTTGCTCC ATACCCTGGTGTGTTAAGGAACTTCACAGGAACTCTGACCTTGAAGTACTTGAGATGCTCAAATGTGCCC AGCCTGTTGCTTTATGTTTTAGTGTGTCAGCATAAATATCATAAGCAGATTTTTAAAAGCCTGGATATTC TTAATTCAAGGATAAAAATGAAGAAGCTTGTGGTATATATACATATATATATAAAGCTCAAGATCCAACT GAAATATTAGTGGAATTTTCTACTGACTCATTGGACTGAAAGATGAAGTATCTGTGGAAATAAAAAGCCA AATTTCTTGTTGCTACAGGATATGACAACAATGAAAAGGATCTTGTATTTTAAAAGAAAAAATGTAATTT TTATAAAAAGAAAACTTGTTTTTCATTCAGAATGGTCATTTTTACTTTGGTAACTTTTTCATAGGTCCTA AAAGAAAACTGTTTTTTGAGAAACTATTGTTAAGTACCCTTCCTGCATCCCTTTTGCCTTCTCCTCTTTC CAAATTCTTTCTACAAAAGAAAAATAACAACCCTCAATGCTGGAAAACCCTTTGCCTACTTTCTGTAAGT CAACTCATCAGGAACTACTTCCAAGAGATGCCTCCATTTCCAGTCTCAAAACACCCACGCCACACGGCAG CTTACCACAGAAGTTTTCATCCCAGAATTTTCTAAAAGTTATAAAAAAGACCATGTTGAAAACAAAACAA AACCCAGCCCCTTGCGCGTGCTTTCCTCAGAGCTGCTCGCCCCTGGTTGTGCTACCAAGCAACCCTCCAG AGCAGCGACTGGGTGTAGATGACTGAATGTTACTGAGGTTGCAAGTGACAATCTTAAACTTTGCACTCTT GTATGTATTTCTCATTTCTTTTGGAAATAGTTTCCAGGAAAGGCTGTTATGTCTCCTGCTATGATTTGTA TCATTTAAGCTTCTGTTCTTGTTTTAAATTGAGAGCTCTCAGCCGATCGAGCTGGATCTTAACAACGAAC TCTCCCCTCCTGTCTCCCAGGCTCTGCCATTTTCTCTATTTCTAGTGTTAGACACACTCTGAGTGGTATT TTCTGTGCACTGAAGCAGCCTATTTTTAAAATATTTAAGGAGAAGTACCTTAGCTCATGCTTGTACTAGT TCCCTTTCTGTTGTTTAGCTTCGGTTGAATTACAAATTCTTTGTGCATTCTTGACTTTGCCTTATTTTAT GTAAAATCTCTGTCATTCGGGTTTTGACAATGAGTTGAAGGCATTAGTGGCATTCCTTACTCCTTACATC TAGTTTTTCAAGTACTGACTGTGATTGTGACCAGGTAATGTGCACTTTTTCTTGTAACTGTGGACATTGC TATGCTTTTTTCTTCTAGTGTTTCTAGAACTATTGTTCCTTACAGTTACGTAAATAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6850243 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6850243 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826744 same experiment
	EurExpress:euxassay_008266 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source