EMAGE mouse gene expression spatial database

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EMAGE:12230

Slc35f1 solute carrier family 35, member F1 ( MGI:2139810)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008274. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008274_01	euxassay_008274_02	euxassay_008274_03	euxassay_008274_04

euxassay_008274_05	euxassay_008274_06	euxassay_008274_07	euxassay_008274_08	euxassay_008274_09

euxassay_008274_10	euxassay_008274_11	euxassay_008274_12	euxassay_008274_13	euxassay_008274_14

euxassay_008274_15	euxassay_008274_16	euxassay_008274_17	euxassay_008274_18	euxassay_008274_19

euxassay_008274_20	euxassay_008274_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12230_wholemount_strong.wlz

12230_wholemount_moderate.wlz

12230_wholemount_weak.wlz

12230_wholemount_possible.wlz

12230_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12230_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9152
Entity Detected:	Slc35f1, solute carrier family 35, member F1 ( MGI:2139810)
Sequence:	sense strand is shown >T9152 AGCCCTAGGCACTGGGAGAGAAGACGAAACCCGGCAGCCCCGTTCCTCTCTCTGCACGGTCCTTGCAGTG GACCCGGCAGCTCCGGGTCCCCGCCGACCCCCTTTGCAGCCACTTGGCGGGGTGTGCCAGGACTTGGTTG CAGCTCCGGCGGGAGGTGGCAGCGGCCCGGAGAGAGGGTGTGCAGCCAGGACTGGGTAGCAACCCGAGAG CACTGGGCTGGGAGGAGGCGGCCGGAGCCCGCCGGTGTCTCCCGCTTCGTCGCTTCGCAAGCGGCACCGC CTCCCCGGGCCGGGGCCGCTTGGCCGGGTCCTCTGCTTGTTCCCGGGCCCGGAGCCCGCGCACTATGTGC GGGGCAGCGTTCTGATAGCCATCCACCTCCGCCTCCGCCGCGATGATTCCCCCCGAGCCTCCGCAGCCCC AACTGCAGCCACCACCTCCACCGGCCCCGCCGAACCATGTGGTGACCACCATCGAGAACCTGCCAGCTGA GGGCAGCGGCGGCGTGAGCCTGTCCGCCTCCTCCCGGGCTAGCATGCGCCAGAGGATCCGCAAAGTGCTG AACAGGGAGATGTTAATCTCTGTGGCTTTGGGTCAAGTGTTATCCCTTCTCGTTTGTGGAATTGGCTTGA CGAGCAAGTACCTGGCAGAGGATTTTCATGCGAACACACCAGTCTTCCAGAGCTTTCTGAACTACATCCT CCTCTTCTTGGTATACACCACCACCCTAGCTGTCCGACAAGGAGAAGAAAACCTCCTGGCAATTTTACGA CGAAGATGGTGGAAGTATATGATCCTTGGACTCATAGACTTGGAAGCTAATTACCTGGTGGTCAAGGCTT ACCAGTACACAACTCTGACCAGTGTCCAGCTCCTGGACTGTTTTGTGATCCCGGTGGTGATTTTGCTCTC CTGGTTCTTCCTGCTGATCCGGTACAAGGCTGTGCATTTCATCGGCATCGTAGTCTGCATCCTGGGTATG GGCTGCATGGTGGGAGCGGATGTGCTCGTGGGAAGGCACCAGGGAGCAGGAGAAAATAAGCTGGTAGGAG ACCTTCTGGTCTTAGGAGGAGCCACCCTGTACGGGATCTCAAATGTCTGGGAAGAATCCATTATCCGAAC CCTGAGCCGTGTGGAGTTCCTGGGAATGATTGGTCTCTTTGGTGCATTTTTCAGTGGAATTCAATTAGCC ATCATGGAACACAAGGAACTGTTAAAGGTACCATGGGATTGGCAAATAGGACTGCTCTATGTTGGCTTTA GTGCCTGCATGTTTGGTCTCTACAGCTTTATGCCAGTTGTCATAAAGAAAACGAGTGCCACCTCGGTCAA CCTTTCTCTGCTCACAGCAGATCTGTACAGCCTGTTCTGTGGACTCTTTCTCTTCCACTACAAGTTTTCA GGTCTTTACCTCCTGTCTTTCTTCACCATCCTCATCGGGCTGGTGCTGTATTCCTCCACCTCTACATACA TAGCCCAGGATCCCCGAGTGTACAAGCAGTTCCGCAATCCTTCAGGACCTGTCGTGGACTTACCCAGCAC GGCTCAGGTAGAGCCTTCAGTCACCTACACCAGCCTGGGCCAGGAGACAGAAGAAGAGCCCCATGTACGT GTGGCCTAGGGGAAGACCTGCCCTGCCCACTGAGGACAACTCAGAGGCCATGTGTTTGCCCATCATCTGT ATTGTACATAGAGAAAGGTATTTAGTAGGTGCAGTTTCACAGGTGGACTGCAAGGTAGCTAATCCTTTCA TCAAAGGAAGAAGCTGAAGACCACTGGAGACTCAGGCTTCAATCCACCTGACTCAGAGAGAGGTTTTGAT AGTGTGTATCCTGACCCAGTCCCCACCCCTCCATTGATTACTGTGAAAATTTTTGAATCAAAAACAAGTA TCATTGTTACTATGATCTTTATTATTAATTGTTACTGTTATTACACGACTTTCAGAAGGTTTTTTATTCC TGATGGGAATTATTACCAGACCCTCACCTGCTCAACATAAATCCCACTTTTCTATGGCAAGTCACTTTTT AAGAATCATACTTTTTGTTTGTTTGTTTGTTTGTTTGTTTTGCACAGACTATGTGAGTGATGCATGCCAC AGAAGCTTGCCCATCCTGGTGACCTTGTGGCTAATGGTGTCCTCTGTCACCTGTTGGATTGCCTGTTCGA TGAGGATTTTGTGCTGTGACCCTTGTAGGGAGGGGAAACTGACCATACAATTCTCTATTCTAGGAATAGA TATAAAACGAACATTATAGCCATTTTATATTTCATTTTCTGCTTATTCCAGGCCACTGGCTTTCTGCTGG CCTAGGAGATTCATCTAACCAAGCCCTGTAACTACATGATAGATAGGATAGAGACTGTCCCATTTTTGCC ACTTAAAGAAACCCTAGAAATATGGGTTATATTTAGGGTTATATGTTTTTAATTTCTTTTTCTGTCTTTC TTTATATCGATCTCCTCAAAAATTAGTTTTTATTTTCATTTTTGTTCATAATGATGTCATGGAAATTAAT CAAGGGACTGGAAAAGCCATGACTTTCACCCTCTTATTTACCTTTCTGGAAGTATGCCAACATGGAAATT ATCTTTATTGTGGAGTCCCACTGTTCGTCCCACCTCTTTGAGTCAAGGTACTAGTATGGAGAAATCTCTG TTAGTAGCTCGTGCTCTGGTCACTTAGCGATAAAAATGTCACCATTGAAAAGTCTGGCACTTTGATCACA CTCAATACCCACCTAATAGTAGAATATAAGGAAAAGCAACTCTACGCACAAAGAATGCTACAGCATCCCA ATAAAGCTTAATACATCACTAGTTTAATCAGTTGTCTTCAAGAATATTTCCCCCACACCCACCCCACTCC AAACACAGAAAATTAAGAAATACCACAAACCTTTATAATGTAGAAAAATTTCACGTGTCAGTGTGAAAGT TCTCTGTAGCTTGGCTGACTTCAATACCATTCAAATGGATCCTTGATGGAGTTACAGGGTTTGTATAAAT GTTTATGTAATTTGAGGCAATTCAAGTCAACATATTTTAAGCAAGCAAGCAAAACAAAACACTTGTGGGA GGTGGGGGTCTCTCATAAAGTAAGACCAGATTGCAAAGTAGGACCTCAACAAGTCATAAACATGGCTGGC AGAAGCTCTTCGGCTGTCTACCAGACTCTTTGCCTGCCTTCTTCTGTGAGCTATTTTGATAAGTCTTATG AGAACAGCACATACACAAAATAAGGAATGCGCTTGAACACTGTAACTCAGTTTTAGGTTTTTTTCCATCG AAAAATATAACATTTCCCTCTAACATATTGTTTAGTCCTGTCCTAGGAGATAGAAAATAAAACTATTTTC CTGGTGTGCAAATTTCACATCAGTTCAACTCCTTTGAAAATAGGTTTTCTTTATTAGGGTGGAGTGGGCA GGGGCAAGAAAGGTTTGTGCTAAAGCTACATCACATAATAACCTTGCAAATATCTGCTCTGTGCTACCTT TTAAGCCAATTAAATATCTTTCTCTGAATATTTTGTCCATTCAGATCTGAAGACCCTTAATGACTGTGGT TTTACTTTTATGTTTACATTCCTTCAGTTTGCATAATTTACTTGATTTATTGCATTTTTAGTATTTATTT TAAGCCAAATGTCTTTGTCTTTTATTCATTTTTATGACACTAATTTGTAGACACTCTGTAAACTCTAATG AGAAACAAATGGCTGAGATTATTTCCAAGTCCATTCAGTAGGCTGGGAATAATTTTAAAGTTACATTTTG AAAAGGTTTGCAATTAATTGATGGCTAAAGATGGCTGTCTGGACCATTTCATAGGATGAAATTGACTCCA TTGTATGGAAACCAAATGTGAATATCAGGATGAACTCATTGTATACTTTTTGTCATCCAGTGTCTTTGTG ATCAGACATTAATGCACCTGTGTTGGTTTATCTCCATTCATGAAGCTGGTAACATTGGCTCATCCAAGAC TAGGAGTTTAGGATTGACCCTTAAGGGAAAAAGACAAAAAGTTGCAAAGAAAAATGTTTTTAATCTGAAA GCATTGGTGGTTTGACAGCGGGGCGGGAAGTGGGAATAGGGTAATAGTAACTGTAGCAGTTTTAGTTTCT GAGATTCCGTAACCTCAGTAGACACCAGCAAGAATTCCGGCATTTTAATCTACCACTCTTCTCACCTCAA GCAAGAACAGTCCTCTGTATGAAGTTGATAAGAAGCAAACCACAGCTCTCTTAAACCCTACCAGAGATGA GTGGTGGTTTTCACTTTCTCGGTGGAGACTTTCACTGAGGACAGAGCTTGCCTTGGTCAGACCTACTCAC AGCTTCGAACACATATACAGGTAGACCAAGAAATGGACAAGTTCGCACCACTGTGTCCCATGAAGATATT TCAATGTTTAGTTTAGGTATTGCTAACTTGTGGTATTAACCTTCTGACAAGCTTGTAGTATTGTTTGATT GGCATTTATTTTTGATTTGTAACCATTTAGTAGTTCCCAGCTAGCTATCAGTGCAGATTTTACCCCATGG GTAGGAGTCTATCTTCACACCACATAACAAAGCACACTAATCCTGACACCACGATGGGAAATATGAACCA AAATATGAAAGAAAGAAAGAAAGAAAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826829 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826829 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12231 same embryo
	EurExpress:euxassay_008274 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source