EMAGE mouse gene expression spatial database

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EMAGE:12231

Slc6a1 solute carrier family 6 (neurotransmitter transporter, GABA), member 1 ( MGI:95627)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008275. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008275_01	euxassay_008275_02	euxassay_008275_03	euxassay_008275_04

euxassay_008275_05	euxassay_008275_06	euxassay_008275_07	euxassay_008275_08	euxassay_008275_09

euxassay_008275_10	euxassay_008275_11	euxassay_008275_12	euxassay_008275_13	euxassay_008275_14

euxassay_008275_15	euxassay_008275_16	euxassay_008275_17	euxassay_008275_18	euxassay_008275_19

euxassay_008275_20	euxassay_008275_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12231_wholemount_strong.wlz

12231_wholemount_moderate.wlz

12231_wholemount_weak.wlz

12231_wholemount_possible.wlz

12231_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12231_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

cerebral cortex marginal layer

strong

regional

strong expression: see section 10 11 12 13 14 15

glossopharyngeal ix ganglion

moderate

spotted

moderate expression: see section 06 07 15 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 15 16 17 18 19

spinal cord

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

neural retina

strong

regional

strong expression: see section 01 02 03 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9165
Entity Detected:	Slc6a1, solute carrier family 6 (neurotransmitter transporter, GABA), member 1 ( MGI:95627)
Sequence:	sense strand is shown >T9165 GAGGTTGCAGGCGAGCTGCGCGAGCCCAGGAGCCCAGGCGCCCGCGTCCAAGCGCGGCCGAGCCCGGAGC GGCCCGCGCAGCCCCGGCCGCAGGCTCTGTGGAGAAAGCCTTTAGGAGAAGACTCCTAGCAGAGATCGAT TAGGCTGCAAAGCTGCTGTCCACGTGGACTGGAGCTGACATCTCGCGCCCACCTGCCAGGATCCCCGCTG CCAAGTTTGTGCTCCGAGACATGGCGACTGACAACAGCAAGGTGGCTGATGGGCAGATCTCTACTGAGGT CAGCGAGGCCCCTGTGGCCAGCGACAAGCCCAAAACCCTGGTAGTCAAGGTGCAGAAGAAGGCCGGGGAC CTCCCTGACCGGGACACATGGAAGGGACGCTTCGACTTCCTCATGTCCTGCGTGGGCTATGCCATCGGCC TGGGCAATGTGTGGAGGTTCCCTTACCTCTGTGGGAAAAACGGTGGCGGGGCCTTCCTAATCCCATATTT CCTGACGCTCATCTTTGCGGGTGTTCCTCTCTTCCTTTTGGAGTGCTCCCTAGGCCAGTACACCTCCATT GGGGGCCTGGGCGTATGGAAGCTGGCGCCCATGTTCAAGGGTGTGGGCCTCGCGGCAGCTGTGCTGTCCT TCTGGCTGAACATCTACTACATCGTCATCATCTCCTGGGCCATCTACTACCTGTACAACTCCTTCACCAC GACCCTGCCATGGAAACAGTGTGACAACCCGTGGAACACTGACCGCTGCTTCTCCAACTACAGCCTGGTC AATACCACCAACATGACCAGCGCCGTGGTGGAGTTCTGGGAGCGCAACATGCACCAGATGACAGATGGAC TGGACAAGCCAGGACAGATCCGCTGGCCTCTGGCCATCACACTGGCCATTGCCTGGGTGCTCGTGTATTT CTGCATCTGGAAGGGTGTTGGTTGGACTGGAAAGGTGGTCTACTTCTCAGCCACGTACCCCTACATCATG CTTATCATCCTGTTCTTCCGTGGAGTGACGCTTCCCGGGGCCAAGGAGGGGATCCTCTTCTACATCACAC CCAACTTCCGAAAGCTGTCTGATTCTGAGGTGTGGCTTGACGCCGCCACCCAGATCTTCTTCTCCTACGG GCTGGGCCTGGGGTCCCTGATTGCTCTGGGAAGCTACAACTCTTTCCACAACAATGTGTACAGGGACTCC ATCATCGTTTGCTGCATCAACTCCTGCACCAGCATGTTTGCCGGATTCGTCATCTTCTCCATCGTGGGCT TCATGGCTCATGTCACCAAGAGGTCCATAGCTGATGTGGCAGCCTCAGGCCCGGGGCTGGCATTCTTGGC GTACCCTGAGGCTGTGACACAGCTACCCATCTCTCCCCTCTGGGCTATCCTCTTCTTCTCCATGCTGCTG ATGCTGGGCATTGACAGCCAGTTCTGTACCGTGGAGGGCTTCATCACTGCCCTGGTGGACGAGTACCCCA GACTTCTCCGCAATCGCCGTGAACTCTTCATTGCTGCCGTGTGCATCGTGTCCTACCTGATTGGCCTGTC TAACATCACCCAGGGTGGCATTTATGTCTTCAAACTGTTTGATTATTACTCTGCCAGCGGCATGAGCTTG CTGTTCCTGGTTTTCTTCGAGTGTGTCTCCATTTCCTGGTTTTATGGTGTCAACCGGTTCTATGACAACA TCCAGGAGATGGTTGGCTCCAGGCCCTGCATCTGGTGGAAGCTGTGCTGGTCCTTTTTCACACCCATCAT TGTGGCGGGCGTGTTTCTCTTCAGTGCTGTGCAGATGACACCACTCACCATGGGAAGCTATGTTTTCCCC AAGTGGGGCCAGGGCGTGGGCTGGCTCATGGCTCTGTCCTCCATGGTGCTCATCCCCTGGTACATGGCTT ACATGTTCCTCACCCTGAAGGGCTCCCTGAAGCAGCGTCTCCAGGTCATGATTCAGCCCAGTGAAGATAT TGTGCGCCCTGAGAATGGCCCTGAGCAGCCGCAGGCTGGCAGCTCAGCCAGCAAGGAGGCCTACATCTAG GGGTGCAGCCCCCCATCACCCCTACACTGGCACTCTGGACTGGCTGTACCCACACCCCTTGAAGACTGAA GATACTCTCTGTCTCCACCTACCTCAAGGGGCAGGTCCAGACACCATGACCATGCAGAGAGGGGCGGTGG GGGACAGTCTGACCCTGGGTGGGCCCTGAGTGGGCAGCCGCCTCTGGAGGCTTCCGTAGAGGCCCCTTAG CAGGAGCAGGTGGCTAGCCTTTGTCACTGCCACTGTAGCTCCTTTTTATGCTGCCAGAGAGGGTGATAGC TGCGGCCACACGCTCCTGGCTTTTAGTCTTTTTTTTTTTTTTTCAGACTGTTGTCCTGTGCCCCAACTAT AGACTGTTATCCAGACTTTTCTGCCCGCCTTGGCTCTGAACTGGTCATGGACCGGGGCTTCGGCATTTTG TTCATGGCCGCTGGGAGCCAGCAGGCTCTGCCTCTTCCTTCTCAATTTCTCCTTCCTGAGGCAGCTTCCA GGACCCACCCCCAGACTGTTCACAGATCAGTGCGGAGAGGGTCCTCTGTCCCTGTGACCCTGTGACCCGG GCAGCTCAGTGTCCCCTGCCCTACCTCCCCAGGTGGCATTAACAACAACAGCCCATCCAGAAGGGTCCTG TGTACTGGGAAGGAACAAAAGAAATCACAAGCACAATTGCCTTTTTTGGTCACCATCCCAGGACTTTCCC CAAGTGGGAGCTGTGGTCCTTCTGAGCTGCCCAGTTTGGCACAAACAATGACCAAGGAAATCAAGGTGAA GGTTCTAAGTCAGGACCAATCCAGCTCACTCTGGTTTCCCTCTTAGACTGTCACTGCCACTCTGTTCTGG TGTCCCCCCTCACCTTTCTGGAAAACTTTCAGATGTACACGCCCACTCATGGAGCAGGGTTACTCCAGAG GCTCCGCCCACCCTACCCTGGCATTAAGCTTCCCTTGCTAGCCTGTTCTAGTGGGGACATCGCATGTCCC TTCTGGCCTTGGGTTCTGTGTTGGTGAAGCCAGCGGAGACAGTTTCTGGAAAGTTCCAGAAGCTCTGTCT CTCTCCTCTGCGGAGAGGGTGGACTCCCATGTAGCAAAGCGTATGTCTCGTCCCGTAGCTTCTTAGCTTG ATAGCTCACAAACTGTGTTTATGACTAATCCTTAATAACTATGGTGAATAACTGTGACCGTGGGTTTTTT GAATCTCTTGTCATTCTCATCCAGAAGTGACCAGCACACCAGTTCTTGCAATAAGCTATCTCCCTCCCTC AGAGCATTAAGCACACTGTAGAGAATGCAGACACGTATGCACATAGAAATGCACACACATGCATACCCAT CCTCACATGTGGCATTTAGCGTCCCGTGTGATATTGTGTAGGCAATCTACCAGCTCTTCCCGAGGCCACT TGTAACAGGGTTGTGTGGCTGAGGCATCTGCCTGCTCGGTGGAGACTCTGCGACTGACCAGCGTGCACAG ATGCCTGTCGACAGAGTCCCAGGTGTGGAATGCAAGGACCCTCCACTGTGTCTCGTGGCCTCAACCCCAC CCCACCCCCACCTGTGTAGGAAGCCCTTTAGGATGAGGGCAGGAGGTCTCCTTCTTGCTGCTCGGTGTTC TTTGATGTGAAACTGAGAACAAGTCTTTTTGAGATAAATGCAGTGTATTTCATGTTTGTAAGCACCTCTG AGATGTTTGGCAAGAAATCCCCTGATTTCCACCCAAACTTACCTTATAGAGCACAACGTTAAAGGTCGTA CAATTACTGTGAGAACTGTGAATATGTGTAACTTTTTTTTTCAGTTTTTGCCCAGAGGGAAGAAGATAAT TGTATTATCATATATGCTTTCTTTTTTTTGCAATAAGGATTTATTCTCAGAACACCAAGTAAATTTATCT CTATATAAAAGTATATGTAATATATGCCTATTCAGACTATATACAGAGCCTGTTTTAAAAAAATTACAGT ATTATTTAGTAAAATTATCTGTTCTATGGACCAAATGTAAAATATTTATACATGAAGATGTGTTTTAAAT GTCTATCAAATGGAATCACAGCTAGAACACGGGCGTCATGTACGTTTCTAAGAATTTAGAGGAAAAATAA TAAAGGTTCTATGATAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827121 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827121 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12230 same embryo
	EurExpress:euxassay_008275 same experiment
	MGI:4828258 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source