EMAGE mouse gene expression spatial database

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EMAGE:12234

Lgi1 leucine-rich repeat LGI family, member 1 ( MGI:1861691)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008470. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008470_01	euxassay_008470_02	euxassay_008470_03	euxassay_008470_04

euxassay_008470_05	euxassay_008470_06	euxassay_008470_07	euxassay_008470_08	euxassay_008470_09

euxassay_008470_10	euxassay_008470_11	euxassay_008470_12	euxassay_008470_13	euxassay_008470_14

euxassay_008470_15	euxassay_008470_16	euxassay_008470_17	euxassay_008470_18	euxassay_008470_19

euxassay_008470_20	euxassay_008470_21	euxassay_008470_22	euxassay_008470_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12234_wholemount_strong.wlz

12234_wholemount_moderate.wlz

12234_wholemount_weak.wlz

12234_wholemount_possible.wlz

12234_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper leg muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

neural retina

moderate

spotted

moderate expression: see section 01 02 22 23

nasal cavity olfactory epithelium

moderate

spotted

moderate expression: see section 08 09 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9281
Entity Detected:	Lgi1, leucine-rich repeat LGI family, member 1 ( MGI:1861691)
Sequence:	sense strand is shown >T9281 CAGAGAAGGGTGGACTCCTATGTGACCTGTTCTTAGAGCAAGACAATCAGCAGCTGAATTCCAGAAGCCC TGTTCATGTGTGGGGATATTCTCTCGACTGCATGGAATCAGAAAGCAGCAGAAGGATGGGAAATGCCTGC ATTCCCCTGAAAAGAATTGCCTATTTCCTATGCCTCTTTTCTGTGGTTTTGCTGACTGAGGGGAAGAAAC CAGCGAAGCCAAAATGCCCTGCCGTGTGTACTTGTAGCAAAGATAACGCTTTATGTGAGAATGCGAGATC CATTCCACGCACCGTTCCTCCTGATGTTATCTCACTATCCTTTGTGAGATCTGGTTTTACTGAAATCTCA GAAGGGAGTTTCTTATTCACACCATCGCTGCAGCTCTTGTTATTTACGTCGAACTCCTTTGATGTGATCA GTGATGATGCTTTTATTGGTCTTCCACATCTAGAATATTTATTCATAGAAAACAACAATATCAAGTCCAT TTCAAGACATACTTTCCGGGGACTCAAGTCTTTGATTCACTTGAGTCTTGCAAACAACAATCTCCAGACA CTCCCAAAAGACATTTTCAAAGGCCTGGATTCCTTAACAAATGTGGACCTAAGAGGGAACGCATTTAATT GTGACTGTAAGTTGAAGTGGCTGGTGGAATGGCTCGGCCATACCAATGCAACTGTGGAAGACATCTACTG TGAAGGCCCACCGGAATATAAGAAACGGAAAATCAATAGCCTCTCCCCCAAGGATTTTGACTGTATCATT ACAGAATTTGCAAAGTCCCAAGACCTACCTTATCAGTCGCTGTCCATAGACACTTTTTCTTATTTGAATG ATGAATATGTAGTCATTGCTCAGCCTTTTACTGGAAAATGCATTTTCCTGGAATGGGACCACGTAGAGAA GACCTTCCGGAATTATGACAACATTACAGGCACGTCCACTGTGGTGTGCAAGCCCATAGTTATTGACACT CAGCTCTATGTCATTGTGGCCCAGCTGTTTGGTGGCTCTCATATCTATAAGCGAGATGGTTTTGCAAACA AATTCATAAAAATCCAAGATATTGAAGTTCTCAAAATTCGAAAACCCAATGACATCGAAACATTCAAGAT TGAAGACAACTGGTACTTCGTTGTCGCTGACAGTTCCAAAGCTGGGTTTACTACCATTTACAAGTGGAAT GGAAACGGATTCTACTCCCACCAATCCTTACATGCCTGGTACAGGGATACTGATGTGGAATACCTTGAAA TAGCCAGACCACCATTGGCCCTCAGAACACCTCATTTAATCCTGTCCAGTAGTTCTCAGCGCCCTGTTAT TTACCAGTGGAGCAAAGCAACACAGCTATTCACTAACCAAACCGACATTCCTAACATGGAGGACGTATAC GCGGTGAAGCACTTCTCTGTGAAAGGTGATGTGTACATCTGCTTGACCAGATTCATTGGCGACTCTAAAG TCATGAAGTGGGGCGGCTCCTCCTTCCAGGACATCCAGAGGATGCCATCTCGAGGATCCATGGTGTTCCA ACCTCTTCAGATAAATAATTACCAATATGCAATTCTCGGAAGCGATTACTCCTTTACTCAAGTGTATAAC TGGGATGCAGAGAAAGCCAAATTTGTGAAGTTTCAGGAGTTAAACGTTCAGGCACCCAGATCATTCACAC ACGTATCCATTAATAAGCGTAATTTTCTCTTTGCTTCCAGTTTTAAGGGAAACACGCAGATTTACAAACA TGTCATAGTTGACTTAAGCGCATGAGACACCAAACTCCACGGCTGCCATCGGAAACTTTCTACAGTGCGT GACCTGGATGAACTCAATGCATGATGACTCTTCTTATCACACTTGCAGATGAATACCTTTCAACACTGAG ACTGCTAAAGCCAATACTACCAGTATCACCATCCTTAACTGTCCGGTCCAGTGGTGTGGGAAGTTACCTT TTATAAGACAAAAGTTTCCTTATGTAACTGTTCTTTGCAGTGAAGATGTGTAAATAAGCGTTTAATGGCA TCTGTTACTCCGAAAAGAAATATTCATATGTACTTTTCCATTTATTTATTCACGTGTTCAGAAACAACTG CCAAATAAAATGTTTACATTTTCTTTCATATCCCAAAGGTAATTATTTACAAGAGCTGTTTGATTCCTGT TGATTGTATGTTGAGGAAATAGTTTTATACACTAGGATTATATTTGGATCAAGGATTTATTGTATGAATA TTGAGTAAAGATAACAAATGAAAATAATTTCCCATAATTTATAAGCACCTTTATCCGGGTTCTATCCGTG GGTTTTGAATACTCAGAAGTCACGACGTTTAGGGACACTTAAAGGTGAAAGGGTAACACCCTTCAATGAG AGGGAGTTTGCCTATTATGATAAGGCTAACCTGTAATGAGAAGGAAGACCACCCTGTTCTATCTGATAGC CTGGGTTAAATACCACTCAGCCATTTTATAGAAGAAATTTCAGATTTAGGATTGAAAAGCTCCTGATCTG GGACCTTCTGAATATCTAACTCTAGAGTCTCCTGACAGCTGGTAGATTGTAAATTATGGAGAGCACTTGC CTCCCAACATGCTGGCCCTGGAGGCCTCTCTATCTGGCAGAGGACATTTTGCTTCTAGACACTGGATTTG CTGTGACTGACCTGCCACACAGTGCCTGTGAGGCTCATGTTTGATTTTCTAGATTCCTGCTTCTGGATCA CTTGAGTCATTAGTAGCTAGCACAATGCCTGTTATGTACATGTAGGCTCTCAAACATTGCTTTTCAAAGG CTGAGGATATAGTTTCACGGAAAAGTACTCAATGTGGCCTGTGTAAAACCTGGAGTTCAATTCCCAGCAT AAAACAAAAGAGTTTGCGGGGGTGGAGGGGGAACGAGTGACTCCTTCTCTTATAATAAACTCCACGGGTC CCTCTGACCTATCGAGTACTTACTGGCTACTTGTTCAGAATATGGTGTGGTTGTGGGTAACTAATACAGG AGGAGGGTGCAATGCTCACCCTCTGTAGTCTTGAAAAAGATTGGCAGCGGGCTAAATATACAAAAACATC TATAGATTAAGCAATGTGTACAAAGAATGACCTGAAAATTCTAGATTTTGAGTATCATGGGAACAACCAG AGCAACTTTGGGGAAGCTATGCTCTTCGTCTGACTTAAAGGGAAGAGGCAAAAGAAAGTGTGTAGGGAAG ACATTGCGGGCACAAACTGGTTATGTGTGCTGCCTCTATGGAGAGCTTTTGAGCTTGCTATGTTCCCACT GGATGGAGGTAGGCAGACAGCAGTACCCTCTGCCCAGGATAAGTCTGGTTGCCTGGAGCATCAGGGGTCT CCACAATAATCACACAGTCTCCAAAGAAGCAACACCCTGGGTCACCACGCACAAAGACAGAATTTCATTA CTCATCTAGAAAAGATAGTAGATTTGGCTTCAGAGTGATAAGGAAGAGGAAAGCTTAACTCCTGATCATT CGCTGGCCTTGGAATGCCTTTTCCTTTGTTCTAATAAGCAACATCATCATATGCTGCTGGAGGACGCCTG CACTGTACAGTCGGAGTTGAAATCTAGCTCTGCCATGCACAGGCTTTGTAACAGGAAAGATCTTAACCAA CCTGAGCCCACTTCTTCATTCGTAAAGATGGATAACAAAACCTACTTCTGAGAAGAGGGATAAATGAGAA AACCCGTGAACAGCAGATGGTATTTTTTCCAAATACATCCCCTACCGCACCTTGAACCCCCACGCCTGTG AGACCTTGTCCAAAATTCACTAAGAGGTAGCATCGAACTCACCTGAATGGTTGGTGACTTGCTGTTAATA CGTTGAATTTGGTGAAAGTGATAGTTTGGCTTCCACGCTAAGTCATAAAAACCATGAACTCCTTCTTGCC TTGCAAATTGGAACACTTGCCTGCAGAGCCCAGAACATCTGCATAAGAAGTCTTTCTAACCAGAGGCCAT TCTTCTTTGTTGAAAACCAATCCCCTCGGGAGCAACCACCTCCTGGTGCTCTGGTCAACAGGCCCAGCTG GTGTTGCAGCCAACAGCCATCATCAACCACCAGTCCTGTAAAGCCTACAGCTCCAGACCGTGTGGTCAAG TTCCTACCAGACATCAGACCTTCCCACCTAAGGGGCAGCCATTAAAGAGCAGCAAGCCATCCACAATATC CCTGCCCAAATTTGTGACCCGGACTCTATGAGAATAATAAAGTGGTTGTTTTAAATGGAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839091 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839091 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12233 same embryo
	EMAGE:12235 same embryo
	EMAGE:12236 same embryo
	EMAGE:12232 same embryo
	EurExpress:euxassay_008470 same experiment
	MGI:4825911 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source