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EMAGE:12235

Kcnq3 potassium voltage-gated channel, subfamily Q, member 3 ( MGI:1336181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235
"Pseudo-wholemount" of euxassay_008387. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008387_01 euxassay_008387_02 euxassay_008387_03 euxassay_008387_04
EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235
euxassay_008387_05 euxassay_008387_06 euxassay_008387_07 euxassay_008387_08 euxassay_008387_09
EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235
euxassay_008387_10 euxassay_008387_11 euxassay_008387_12 euxassay_008387_13 euxassay_008387_14
EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235
euxassay_008387_15 euxassay_008387_16 euxassay_008387_17 euxassay_008387_18 euxassay_008387_19
EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235 EMAGE:12235
euxassay_008387_20 euxassay_008387_21 euxassay_008387_22 euxassay_008387_23 euxassay_008387_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12235Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12235_wholemount_strong.wlz
12235_wholemount_moderate.wlz
12235_wholemount_weak.wlz
12235_wholemount_possible.wlz
12235_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12235_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 22 23 weak expression: see section 03 04 05 19 20 21
telencephalon mantle layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 20 21 22 23 24 moderate expression: see section 01 02
telencephalon marginal layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 14 17 18 19 20 21 22 23 moderate expression: see section 15
ventral grey horn
weak weak
regionalweak expression: see section 10 11 14 15 16
midgut
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 11 13 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35254
Entity Detected:Kcnq3, potassium voltage-gated channel, subfamily Q, member 3 ( MGI:1336181)
Sequence:sense strand is shown

>T35254
AATGAACCATATGTAGCCAGGGCAGCCACATCAGAAACTGAAGACCAAAGCATGATGGGGAAGTTTGTAA
AAGTTGAAAGACAGGTTCATGACATGGGGAAGAAACTGGACTTCCTCGTGGACATGCATATGCAGCATAT
GGAACGCTTACAGGTACATGTCACTGAGTACTACCCAACTAAGGGGGCCTCCTCCCCAGCCGAAGGGGAG
AAGAAAGAAGACAACAGGTACTCTGATTTGAAAACCATCATCTGCAACTACTCAGAGACAGGGCCCCCTG
ACCCTCCTTACAGCTTCCACCAGGTGCCCATCGACAGAGTTGGCCCTTACGGGTTTTTTGCACATGATCC
TGTGAAACTGACCCGAGGGGGACCCAGTTCTACAAAGGCTCAAGCTAACCTTCCCTCCTCGGGAAGTACA
TATGCAGAGAGGCCCACAGTCCTGCCCATCTTGACTCTTCTGGACTCATGTGTGAGCTACCACTCCCAGA
CAGAACTGCAAGGTCCCTATTCGGACCACATCTCGCCCCGCCAGAGACGCAGCATCACTAGGGACAGTGA
TACACCTCTGTCCCTCATGTCCGTCAATCACGAGGAACTGGAGCGGTCTCCAAGTGGCTTCAGCATCTCA
CAAGACAGAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92974. Forward Primer - name:092974_F_cDNA_Kcnq3, sequence:AATGAACCATATGTAGCCAGGG; Reverse Primer - name:092974_N_SP6_cDNA_Kcnq3, sequence:TCTCTGTCTTGTGAGATGCTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12233 same embryo
 EMAGE:12236 same embryo
 EMAGE:12232 same embryo
 EMAGE:12234 same embryo
 EurExpress:euxassay_008387 same experiment
 MGI:4825729 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS