EMAGE mouse gene expression spatial database

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EMAGE:12251

Patl1 protein associated with topoisomerase II homolog 1 (yeast) ( MGI:2147679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007139. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007139_01	euxassay_007139_02	euxassay_007139_03	euxassay_007139_04

euxassay_007139_05	euxassay_007139_06	euxassay_007139_07	euxassay_007139_08	euxassay_007139_09

euxassay_007139_10	euxassay_007139_11	euxassay_007139_12	euxassay_007139_13	euxassay_007139_14

euxassay_007139_15	euxassay_007139_16	euxassay_007139_17	euxassay_007139_18	euxassay_007139_19

euxassay_007139_20	euxassay_007139_21	euxassay_007139_22	euxassay_007139_23	euxassay_007139_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12251_wholemount_strong.wlz

12251_wholemount_moderate.wlz

12251_wholemount_weak.wlz

12251_wholemount_possible.wlz

12251_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12251_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9140
Entity Detected:	Patl1, protein associated with topoisomerase II homolog 1 (yeast) ( MGI:2147679)
Sequence:	sense strand is shown >T9140 CGAGCCGGTGGGAGACCCGCGCGCGCGGAGCAGCCCGGGGAGTCGGCGCTTCTTTTCCCTCCCTCCCCCC ACTCCCCTCCCCGCTCCCTTCCCCCCTCCCCAAGAATGTTCCGCTACGAGTCTTTGGAGGATTGTCCTCT AGATGAAGATGAAGATGCATTTCAAGGCCTGGGAGAAGAAGATGAAGAAATTGATCAATTTAATGATGAT ACCTTTGGGTCAGGTGCAGTTGATGATGATTGGCAGGAAGCACATGAGCGCCTGGCTGAGTTGGAAGAGA AGCTACCTGTGGCAGCTGATGAACAAACAGGCAATGGAGAGAGAGATGAGATGGATTTGTTGGGTGACCA TGAGGAGAATCTAGCAGAAAGGCTCAGTAAGATGGTGATTGAAAATGAGCTAGAAGATCCAGCTATCATG AGGGCAGTACAGACCAGGCCAGTTTTACAACCTCAACCAGGAAGTCTGAATTCCAGCATCTGGGATGGAT CTGAAGTTCTGAGGCGAATCCGTGGACCACTACTTGCTCAGGAAATGCCTACAGTGTCTGTATTAGAGTA TGCCTTGCCTCAGAGGCCCCTGCAGGGCCCAGAAGATGATCGGGACCTTTCTGAGCGAGCTTTACCAAGG CGGTCAACATCACCTATCATTGGAAGTCCTCCTGTTAGAGCCGTTCCTATAGGCACCCCACCTAAGCAGA TGGCTGTTCCCAGCTTCAACCAACAGATCCTGTGTCCGAAGCCTGTTCATGTTCGGCCCCCAATGCCGCC ACGCTATCCTGCTCCCTATGGCGAGAGGATATCTCCAAACCAGCTCTGCAGTGTCCCGAACTCCTCCCTC CTGGGCCACCCTTTTCCTCCTAATGTTCCTCCTGTACTTAGTCCCCTGCAGAGAGCACAGCTTCTTGGGG GAGCACAGCTACAGCCTGGACGAATGTCTCCCAGCCAGTTTGCACGGGTCCCTGGGTTTGTTGGTAGCCC ACTGGCTGCCATGAATCCTAAGTTGCTACAAGGGCGCGTTGGGCAGATGCTTCCCCCAGCACCAAGCTTC CGTGCCTTCTTTAGTGCTCCACCCCCCGCTACGCCACCTCCACAGCAGCATCCCCCTGGCCCAGGACCTC ACCTACAAAACCTCAGACCTCAGGCCCCAATGTTTAGAGCAGACACAACTCACCTTCATCCACAGCACCG GCGCCTGTTGCATCAGAGGCAGCTGCAGAGCAGAAATCAGCATCGGAATCTCAATGGTACTGGAGATAGA GGAGGGCACCAGTCCAGTCATCAAGATCATCTCCGGAAGGATCCATATGCTAATCTAATGTTGCAGCGGG AGAAGGATTGGGTCTCTAAAATACAGATGATGCAGCTGCAGAGCACTGATCCCTACCTGGATGATTTCTA TTACCAGAATTACTTTGAAAAACTGGAGAAATTGTCAGCTGCTGAGGAAATACAAGGTGATGGCCCTAAG AAGGAGCGTACCAAGCTCATCACCCCTCAGGTGGCCAAACTGGAGCATGCTTATCAGCCTGTGCAGTTTG AGGGCTCGTTGGGGAAGCTTACTGTCTCTAGTGTGAATAATCCTCGGAAGATGATAGATGCTGTTGTGAC ATCTCGAAGTGAGGATGATGAAACAAAAGAAAAACAAGTTCGGGATAAGAGAAGAAAAACCCTTGTTATA ATTGAGAAAACCTATAGTTTACTCCTTGATGTGGAGGATTATGAAAGGCGCTATCTCCTAAGTCTAGAAG AAGAGCGGCCTGCCCTGATGGATGAAAGAAAGCACAAAATATGTAGCATGTATGACAACCTGAGGGGCAA ATTGCCTGGACAAGAGAGGCCAAGTGATGACCACTTTGTACAGATCATGTGTATCCGAAAAGGGAAGAGA ATGGTTGCCCGCATTCTCCCTTTCCTCTCCACAGAGCAAGCAGCTGACATTCTCATGGCAACTGCCAGGA ACCTCCCTTTCCTTATCAAGAAGGATGCCCAAGATGAGGTGCTGCCATGCTTACTGAGTCCCTTCTCTCT CCTCCTCTATCATCTTCCTTCAGTGACTGTCACCAGCCTTCTGCAACAGCTAATGAACCTACCTCAGAGT GCAAGTGCACCAGCGCCCTCCAACTCTCACCTCACTGCTGTGCTCCAGAACAAGTTTGGCTTGTCACTGC TCCTAATCCTACTAAGCCGTGGAGAAGACCTACAGAGTTCAGATCCTGCTATAGAATCAACGCAAAATAA CCAGTGGACAGAGGTGATGTTCATGGCAACCCGAGAACTTCTACGGATCCCCCAAGCTGCCCTGGCCAAA CCAATCTCTATCCCTACAAATCTAGTGTCCCTTTTTTCTCGCTATGTTGACCGGCAGAAACTGAACTTGC TGGAAACAAAACTGCAGCTGGTTCAGGGGATACGGTAAAAGATCTCCAGAGTATCCTGTACCTCCTGTTG GCTGCCACCTGCACTGCTGCCATCACCAATGGAGTGTTTTTAATGAGGGAGGGGAGGTAGCTTATGACCC AAAGCAAAGTCTTGTGGGATCCATTCCTGTTTTCAGAAGTTAGCTCTGAATGCCAGGTATTCCCGCTGCT CTGATACTTCTGCTGGGTGGTACTTCTGCTGGTTTCTTTTACCTTCTGCGTTACACTTTTGCATGTCCTA CTTTTACTCAATTCTTCTCAATTTTGCATTTTCTTTGCCCTAGAGACACAACAAACGTCCCTTTATCCCA TCACCACTATTACAATTCAAAGTAGGTTGTAGTATACCAAAGGACTCCTTCCCGCTTTGAAGTCTTTTTT TCATCTCCCCTTATTAATATGATTATAGGAGAGTCAAAATTAAGTAGAAGTCAAGATATAGGTAAATATA TAAACACCCTACACAAACATTCGTATGATCTCCAGAGGGTCCTTAAAGAATAAGTTTGTTTGAAAAATAT TTAGTTAACATATACCTCTAAAAATAAACCAGCCGATATATTTTTAATCTGTTTCTGTTCCATCTTGTCA TTTGGTGGATTCTACTTTTTTAATATATAAATACAGGGTATACAGCATATTACATTAAATCAAACTTGTT AATTGGCTGATGCCCAGTTCTAGGCTGAGAGGACTTTTCTTTCTCCTAACCTGTATTTCCTTTGTTTCTG ACAACCATAATAGAAACAGCACACACACGGTTAAGCCTTTTCCTGTGATGACTACAGCGTGCTTGAGAGT CTGTCCCTCAAGTGCATTCAAGAGTACCCAAGATGAGCCTCACGTAGAGCCTGCTTCTGCAGTGTAGCTT TTGTGCGCCTGCACTGTCTTCCTGACGTGACTTCTGTAAGCCATGCTTCTGCTCCTCTTCCCCATTTTCA TTTAGTGTCTTGGGTAGAAACCTCCAAATGTAGCCGTGGCTGCTCAGCTTGGGCTCTGCTCTTCAGTGTC CTCTACCCTGCAGCAGAACTGCTGTCCTGGCTGCTGAGTCTCACCTGAGCCCCATCCACTGCAGCCAGCA GGGCCTGGGTGTGAATCTTAGGGACACTTCCCTTCCACTCACTTTTCTTCCCTTCAGCAGTCAGCCAGGA TAAGGGACTCGGCTCCCTAGTGGTTACAGTTCTGAAAAAAATAAATTGAATTTATTTTATATTCCCTTCC TGATCCTACTTTCTGCTGTTCTCACCTTTTTGAATGTAGTCGGGGGACAGGCTATGATATTTAAAGAGAA TAAACGTTTTGCACATACATGTATTGTATAACAGTAAAGCCCTCCGTTACAACCAGCTGTCTTTGTACTC ATCTCCATTTCTTCTCATTTTGTGGCCTCGGGCTCCACCAGCTGCTCCCCAGTGACAGTCCCTAGCTTCC TTCTACCATCTACTGACTGTCTCCACTGAGCCTTTCCTACCTTCCCTTCACACCCGATCAAGTAAACACT TGATTATTTTTTTCTTCCTTATTGTGCTTTTTGTTCAGTTCTAATTAATAAAATAAAATTTCTAAATTTA CATTTTTATAGGGTATTGTAAATAAAACAAATTGTATACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825758 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825758 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12253 same embryo
	EMAGE:12252 same embryo
	EurExpress:euxassay_007139 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source