EMAGE mouse gene expression spatial database

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EMAGE:12252

Gdap1 ganglioside-induced differentiation-associated-protein 1 ( MGI:1338002)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007164. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007164_01	euxassay_007164_02	euxassay_007164_03	euxassay_007164_04

euxassay_007164_05	euxassay_007164_06	euxassay_007164_07	euxassay_007164_08	euxassay_007164_09

euxassay_007164_10	euxassay_007164_11	euxassay_007164_12	euxassay_007164_13	euxassay_007164_14

euxassay_007164_15	euxassay_007164_16	euxassay_007164_17	euxassay_007164_18	euxassay_007164_19

euxassay_007164_20	euxassay_007164_21	euxassay_007164_22	euxassay_007164_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12252_wholemount_strong.wlz

12252_wholemount_moderate.wlz

12252_wholemount_weak.wlz

12252_wholemount_possible.wlz

12252_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12252_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9141
Entity Detected:	Gdap1, ganglioside-induced differentiation-associated-protein 1 ( MGI:1338002)
Sequence:	sense strand is shown >T9141 CAAGGATGCTTGCTGCAGCAAGTGTCTGCAGAGAGCAGGCCAACCCATAAAGCTGCCTGAGTCCTTTCAT GCGAAATGGCTCGGAGGCAGGACGAGGCGCGGGCCGGTGTGCCCCTGAGGGTTGAAGGCCCGCCGGACAA GGAGGTCCACCTCATTCTGTACCACTGGACGCACTCCTTCAGCTCTCAAAAGGTGCGCTTGGTAATTGCT GAAAAGGCACTGAAGTGCGAGGAACACGATGTAAGTCTGCCCCTGAGTGAGCACAATGAGCCTTGGTTTA TGCGCTTGAACTCAGCTGGAGAAGTACCGGTCCTTGTCCACGGGGAGAACATAATCTGTGAGGCCACTCA GATCATTGATTATCTTGAGCAGACTTTCCTGGATGAAAGAACACCCAGGTTAATGCCCGATGAAGGAAGC ATGTATTACCCACGGGTGCAGCATTACCGAGAGCTTCTTGACTCCTTGCCAATGGATGCTTACACACATG GCTGCATTTTGCATCCTGAGCTCACTGTGGATTCCATGATCCCCGCTTATGCAACCACAAGGATTCGCAG CCAGATTGGTAACACAGAGTCTGAACTAAAGAAACTTGCTGAAGAAAACCCTGACTTACAAGAGGCATAC ATTGCCAAACAGAAGCGACTTAAGTCAAAGCTGCTTGATCATGACAATGTCAAATACTTGAAGAAAATTC TTGATGAGTTGGAGAAGGTCTTGGATCAGGTGGAAACTGAATTACAAAGAAGAAATGAGGAAACTCCAGA GGAGGGCAACCAGCCTTGGCTCTGTGGAGAATCCTTTACACTGGCAGATGTCTCACTGGCTGTCACATTG CATCGGCTGAAGTTCCTGGGGTTTGCAAGACGAAACTGGGGACATGGAAAGAGACCGAACTTGGAAACCT ATTATGAGCGTGTCTTGAAGAGAAAAACATTTAACAAGGTTTTAGGACATGTCAACAATATATTAATCTC TGCGGTGCTGCCAACAGCATTCCGGGTGGCCAAAAAAAGGGCCCCAAAAGTTCTTGGCAGCACCCTTGTG GTTGGTTTGCTTGTAGGAATGGGATATTTTGCATTTATGCTTTTCAGAAGGAGACTTGGCAGCATGATAT TAGCACTTAGACCCAGACCAAATTATTTCTAGGTTTGTTGGGATCTGTGGTAGCAGCACACGGAATCACT CCACTAGACCCTCATCCACTTGTCCAGACCCAGTGAAGATTTAGCCTTGGCAATTAGTAGAGAGCATACT TTATATAATTCACGGGGAGCTCATGTGGGAGAAGGAGCTAATGATATGTTTTTTTCTTCTTTCTCTCTGT CTCTTTCTCTTTCTTCCTTTCTTTTTTTGTAGACAACATGACTTACTTCTAATCTATCCCTAAAGGCCAG TATAACTGCTGACAGATTGGGGGGAGGGAACCTTTGATGGAGATTGTTAGTTCATGTATGTTCTCAGGTA TTTTTTGAGAGGTTGTAATTAGGAGCAGACAGTAATAACAACCCATGATTGCTCATAGTGCCTCACCTGA GAGTGACTGCAGGTCTGGGTGTTGAACAGTTATAGGATTCCTCTGGGACTCAGAGGCATCTTTGTTATAC TAGACAGAGTTCAGACACAAACAAGACATTGTACTGATTCATCTTTCACTAGCAGCTTTAGGCATAAGTA AAGGAGAAAATGAGAAAGGGGAGAAAGAAAATGAGAGAATGAAGTACATCCAGAACAGAGCAGTAGATGC TAACCTCCAGTGGCCTTCATTGCTGCCCATGGTTGCCTCTCTCTTTGGGGAATTTCACATATACCTACTT GTGGTCATGTACCCTCCTTAACTCAGAACTTTTAGGGAGGAGAGGGCTGGGAAGTTAGTGGGGATAGTAT ACTGGGGAGAAACCTATGCACTGCATAGAAAACACTTCATTTGACCTTAATTATGAGTTACACTTGTTAA TTTAATAAGATTAAGTTATTATGCACTTGAATGAGATGCCCATTGAAACATATGTCTAGCACTTTTATGA ATGTCTAAAAGGATAGTAGGGTAGTCTTCATGGCTCCCATGGCCACGTGAGGTGCTCATCCTGTATATGA TTGTCTTCAGTCCTGGGAACCTTGGCCTCGACAGCCCTATTTATGCATACAAGTAATATATAGATGAATG TCTCAAGTAAATATGGCTTATGGATGGTAGTGGATAATTTGATGAGAACTAAATATGCTTAGTTTTATTT GGAGAGACTAGTACATTCATGCACAAACTGTTGGCCAGGCTTTGTATTTGTTTATGATTATTGACAGAGC TTTACATTAAAGAAAAAAGTAAAAGCTAGCATTAGATATTGACAGCTAATTTGGGAAAGGTAAAATATAT AGCTAGCTAGATGTCCACCCATACTCATCCATGTGTGTTTAAATATTTGGGGGATATATCAGTCCATATG GAAGAGACGGTTCATGGATGACCTAGGAATAGCCATCAGACTATAATGCATGATTTTTGCTCAGGACAAG CCTCCTATTAAAGCAGGGAGGCAGTTTTCCTGGAGCTGGCTGTCAAAGCCTTAGGAGAAGCTTCAAGAAT CTGATGGGAAGCTTGTCCACAGACTGTGAGTAAAGAGAAAGTCTGCTTGGATGACCTCAGGTGAACTATG CACATGGCCAAAAAATGTACTGACGTTTATTAATAGGAAAATGTCACAACCAGCAAGCAATGGCTTTCTG ATGATAAAAACAATTCTATAATTCCTTTTTGATTCTATTATTCCATTTCATAAGCTAGATGTACTTAAAA AAAGAACCCATATGAGCAGGATGTGTCCTGTTTGTAAAAAACAAGCAAAACAGCAACATCAAAACAAAAA ACCCTAACACCCCCATGTGAACTCAGATCTATGATGAGGAAATGCACGCAGCTTTTGTGCCATCTTCCTC TGCCCATCAATGTGTCATGCAGAGTGTCTGAATCTGCTTATTGCTAGGACATTGCTGTCATTGTATTTAT ACAACAGTCATAAAATTAACTGACTAGTGATTCAGAATTTCTTACAATGTTCTGATAGAAATAAAAAGTG AGGCACAAAGAAGTAACAATGGGAATTTTGTAAAAAAAAAAAAAAAAAAATCTACAAAAGGTACTTATTT ACTTGTGAAAGGATTGTTAAAGAGTGAATTTTCTTTCAGTATATGTTATGATGGAGTCAGAAAATCACCA GGAAATTATGATTTTCATCTGTAATTTATTTGGAGGTAGATGTGTGCCAGTGTTCAAGGCGAGGCATCCA AGCCTCTTCAATGTTTTATAGGTAGGTATTCTTGACATTAGTGCTTTAAAAATATTTGTCTAGTGATAAA ACCCTGACCACTAGATGTCGCTGTTCACCCAGTAGTCTAAGACTGTTCCATTTGGTTTGGCATGTGTGAG AGGTTTCAAGTGACGATAAAACATTAATGACCTTAGTTTCTGAGGGAATCTTCATTAAGATTAGCCACCT GTGAAAAAGTTTGGGACAAATGTAGTATTGATTTCTTATGCGTTGCGAGTGATGTCAGCACCGTATGATA AGGAGGTGCACAGCCACTGTTAGCCAGAGAGGCACTTTAATGCATCTTCTTTTTAGGATCATGGTAACTG GTCAATATGCTTCTCATGTGAAAAAAAAAAGTATAAATGCCACAGCTAGAAAATGCCCAAGGCATTAACA GGTTATGTTGCCTATTTCTTAACATACATGCACACTATTTTATTGGTACTGCCATTATATGCCTTTTGAT TCTCAAGACTGGTGAACTGCTTGCTCGTTTTAGTAATCATTGCTATTGATATCAAAGTTGTAATGTATTG TTGATTGTCATGGGAAATTAAAGATATTATTCTGATGGTAAGATCAATAAAAATAATTTGAAAATAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825756 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825756 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12251 same embryo
	EMAGE:12253 same embryo
	EurExpress:euxassay_007164 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source