EMAGE mouse gene expression spatial database

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EMAGE:12253

B930095G15Rik RIKEN cDNA B930095G15 gene ( MGI:2443701)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007142. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007142_01	euxassay_007142_02	euxassay_007142_03	euxassay_007142_04

euxassay_007142_05	euxassay_007142_06	euxassay_007142_07	euxassay_007142_08	euxassay_007142_09

euxassay_007142_10	euxassay_007142_11	euxassay_007142_12	euxassay_007142_13	euxassay_007142_14

euxassay_007142_15	euxassay_007142_16	euxassay_007142_17	euxassay_007142_18	euxassay_007142_19

euxassay_007142_20	euxassay_007142_21	euxassay_007142_22	euxassay_007142_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12253_wholemount_strong.wlz

12253_wholemount_moderate.wlz

12253_wholemount_weak.wlz

12253_wholemount_possible.wlz

12253_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12253_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9142
Entity Detected:	B930095G15Rik, RIKEN cDNA B930095G15 gene ( MGI:2443701)
Sequence:	sense strand is shown >T9142 CCAGAGACGTGTTGCTCTCCAAGAAAACAATGGAATGCCCGACCAGAAAGAAGCTAGGGAAGCGCTTAAA ACTCTCAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTCTCCACACAGCCTTGGCTTT GCTTGGTTCTCCATTGAGGTTTTCTTGGGTGACTCTGTTGAGCCCATGATGGCCACTGGGCACAGCCCGG AAGGGGGCTCCCCTGCATGTTTGTGGCTCTGGTATTAGATGGCAGCTTCGCATGCAAGGCAGCACCTGTC CTTCTTCCTAAGGACTCTGGGCCAGGTTAGCTGCTCTGCGATGTCTCCAGATGACAGTTGTTGGTATGGA AAACCTGCCCCTATAGTGGTTGGGATAGAGTTCGAAAGGTCACGTCAGCAAAGCAAAGGTTGGTGCTGCG CCTGTGTGCACTGGTGCCAGGCAGCACTGCAGCCCCCTGGGCTGGTTCAGAATATTCAACAGTGGTGGGA AATGGCAGAAGACAAAGGCCCTTACAGAGTCTGGAGGTGGGAATGGGGCATGCAGGGTTCTGGCCGGTCT GTCATCCCTGGTGATGAGCGTCTGTCTCACGCAGCTGCACCCCCAGCTCCCTTTCCCCTGGCTTCCAGTG TGAGAAGCAAGACCTTGACCCTCTGCCTGCAATGCTACAGAAAGACCACACTTAGCTCTTCAGTTACCAA GAATGCCCGCTCTCTCCAGGAGCATCTAGAGGGCTTGAAGGGTCATCAACCCCTTGCTCTCACCTCTTCC TGCCTTGGAAAATTGCTGCAAACCTCACTAACCGAATCTCACCAGCCCAGCCCATGTTTTTAACACAATT TCCTCCTGTTCTCTCTGTGTGTGCATTTAATCCTTGAGGTAATAATGTGTTGTATTAAAATTTTCTGATG CACTGAATTAATTCTCAAGTAGAAAAGGCTGGGGACTTCATTCTCATTTCTGTCATTTGTTTAATAAATT TAAGAAACATTCTTTCCATTGTTCCACTGTATACCGTTTTGTCAGCATGTACCTACCTGGGAGAAGACGC TCTGTTTTGAACATTCTGTCTTGATCCTTGGAGCATGGCATACCTTCTGTCCCCTGCACTGTTGGAACGC TGTTTGATGTCTTTTCAACCCAAAACCTTGCCTAAATACCAATTTGTACCTGGAATACCCTTTGTAGAAC ACAGCAGTCGCTCACCCAGCGGCACTTTGACAGACCGAATCATGGCCACCCAGGCGCCGGGAGAAGTGTA CACTTGGCTTTTGTGGCCTCATCTTCTCTCAAGGCCCTGCACCGTATAAGGTGCTATGGGGTGGGGCAAG CCCTTGGAGGAGAGCAAGTTCACTTTAAAGGGGGGCAGATGTAGGTGGAACAGAGCGCATCTGAGACAGC AGCTTACGTTGGGAGCTCACACAGCCAGCCGTCCACAGGCTGGGCTTTGCTATTCCCAGTGGGAGTGAAA CACCTGATGCTGATTGGCTTTGGTGGGAGGGAGTGGGGCGTAGCATCTCCATCAGACAAGCTCTTTGTCC CCTGGGTGGGCACCCACAATGTGTGCTCCTCAGATGCGCCCTGCTTTCACTTCTGCCTGTGCTTCCGTGT GGAGCCTCCGCACAGCATGGACTCCTGTCACCCAGGATGCTCTCTGTCTTCTCTGATAGCCATGAGTTTG GTGGCGAGCATGGAAGACCAAACTCATGTCAAATGTTTGCATGGTATTAATATCTTGTGTTACTCTAAAC TCCAGAGGCTTTCCTAAACCTTGTCCTAACGGGAATCTTGCTGTTAACAAACCAAAAACCTGTTTGATGT AACTGTTCTTTCCCACCGATGGCTGAAGCTTTGTCCTTCCTACAAGATGGTATACTAGACTCCACGTTCC TCAGTGACACCAAGTTCACAAGTCCTCCGCCATCTTCAAAGCAGGATTCTCCTTTACCTTTGCCCTAGAA GTAAGAGCAGCCTTGTATGCTCTCTGCCATTTGACCCTAAGTGACACCCCCTCTGTCTGTCTGTCTGTCT GTCTGTCTGTCGCCTGTCTGCAAGTCCGACATCACTGTTGCTTGTCTGAATGGCTTGGTGATGAAGTGTG AAGTGGAAGCTTAGCTGTTGTTGAGCTCGCCTTAAAGGATGCTGGTTCTGAGGAACGCATGTAACTTGCC CCCAGCCACCTAAGGTATCAGGAAGAGGACACCATCTCTAACACTGGTAAACTGTGACCTCACTCTTCCA TGTGGGAACCTTGCCATTCCAGCCAGCTGTTGTCATAGAGTCCGATGGCCAGGGACCCACCTCTGATTCA GTGTGGTGGGGATTTTCTTGGGTGGTGAATGCTCAGAATAGATGATCTCATTCGAAATTTCATTGTCTTC TTTCTTAGTGGGAAGTCGTGAGTTTAAAAAGAGCCCTGTGTTTCTTCTGTCTTTCCTATGTATGCCTCTA GAACTAACACATTCTGAGTATGTAGGTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825849 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825849 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12251 same embryo
	EMAGE:12252 same embryo
	EurExpress:euxassay_007142 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source