EMAGE mouse gene expression spatial database

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EMAGE:12256

Sdhb succinate dehydrogenase complex, subunit B, iron sulfur (Ip) ( MGI:1914930)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007089. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007089_01	euxassay_007089_02	euxassay_007089_03	euxassay_007089_04

euxassay_007089_05	euxassay_007089_06	euxassay_007089_07	euxassay_007089_08	euxassay_007089_09

euxassay_007089_10	euxassay_007089_11	euxassay_007089_12	euxassay_007089_13	euxassay_007089_14

euxassay_007089_15	euxassay_007089_16	euxassay_007089_17	euxassay_007089_18	euxassay_007089_19

euxassay_007089_20	euxassay_007089_21	euxassay_007089_22	euxassay_007089_23	euxassay_007089_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12256_wholemount_strong.wlz

12256_wholemount_moderate.wlz

12256_wholemount_weak.wlz

12256_wholemount_possible.wlz

12256_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12256_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8421
Entity Detected:	Sdhb, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) ( MGI:1914930)
Sequence:	sense strand is shown >T8421 GAGAAACCGGAAGCGGCCTTCCACTCGTTGGCGCTTAGGCGGCTAGCGGTCCTCAGGGTGAGAGGCCGGC TTCCCACCGCGGTGTTCGACGGGATGCCGGAGGGGAAGGGGTGGGGCTGACGTCAGGAGCCAAAATGGCG GCGACGGTCGGGGTCTCCTTGAAGCGCGGCTTCCCGGCTGCCGTTCTCGGCAGAGTCGGCCTGCAGTTTC AGGCCTGTCGAGGGGCACAGACAGCTGCTGCTGCAGCTCCCAGAATCAAAAAATTTGCCATTTACCGATG GGACCCAGACAAGACTGGAGATAAACCTCGAATGCAGACGTACGAGGTGGATCTGAATAAGTGCGGACCT ATGGTGTTGGATGCTTTAATCAAGATCAAGAATGAAGTGGATTCTACTTTAACCTTCCGAAGATCTTGTA GAGAAGGCATCTGTGGCTCTTGCGCCATGAACATCAACGGAGGCAATACTCTGGCGTGCACACGCAGGAT CGACACGGACCTCAGCAAAGTCTCCAAAATCTACCCTCTTCCACATATGTATGTGATCAAGGATCTAGTC CCTGATTTGAGTAACTTCTACGCACAATACAAATCCATTGAGCCTTATCTGAAGAAGAAGGATGAGTCCC AGGAGGGCAAGCAACAGTATCTGCAGTCCATCGAGGACCGGGAGAAGCTGGACGGGCTGTACGAGTGCAT CCTGTGTGCCTGCTGCAGCACCAGCTGCCCCAGCTACTGGTGGAACGGAGACAAGTACCTGGGGCCTGCA GTTCTCATGCAGGCCTATCGCTGGATGATCGACTCCAGAGACGACTTCACAGAGGAACGCCTGGCCAAGC TGCAGGACCCCTTCTCTGTCTACCGCTGCCACACCATCATGAACTGCACACAGACCTGCCCCAAGGGTCT GAATCCGGGGAAAGCGATTGCGGAAATCAAGAAGATGATGGCGACCTACAAGGAGAAGCGAGCATTGGCT TAACCCAGTCCGTGCTGAGTGTGCCTGTCAACTCTGGTGTCAAACAGCTGGAGTCTCCCTAAGTACAGCA TATGTAATAAACATTTAAGATAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5688898 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5688898 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12257 same embryo
	EMAGE:12254 same embryo
	EMAGE:12259 same embryo
	EMAGE:12258 same embryo
	EMAGE:12255 same embryo
	EurExpress:euxassay_007089 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source