EMAGE mouse gene expression spatial database

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EMAGE:12258

Bbs12 Bardet-Biedl syndrome 12 (human) ( MGI:2686651)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007080. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007080_01	euxassay_007080_02	euxassay_007080_03	euxassay_007080_04

euxassay_007080_05	euxassay_007080_06	euxassay_007080_07	euxassay_007080_08	euxassay_007080_09

euxassay_007080_10	euxassay_007080_11	euxassay_007080_12	euxassay_007080_13	euxassay_007080_14

euxassay_007080_15	euxassay_007080_16	euxassay_007080_17	euxassay_007080_18	euxassay_007080_19

euxassay_007080_20	euxassay_007080_21	euxassay_007080_22	euxassay_007080_23	euxassay_007080_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12258_wholemount_strong.wlz

12258_wholemount_moderate.wlz

12258_wholemount_weak.wlz

12258_wholemount_possible.wlz

12258_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12258_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9445
Entity Detected:	Bbs12, Bardet-Biedl syndrome 12 (human) ( MGI:2686651)
Sequence:	sense strand is shown >T9445 ATGGATTTCTAGAACAACTTGAATCAACTCCGAAAGTACTTAGATGTAATGATTTTGGGGAGTTAGCAGT TGGCTTGAGCCATGGAGATCACAGCAGCATGGCATTGGCCAAAGCAGCAGTGAGGTTGCAGTGGCAGAGT CTGTGTCTGCAGCAAGCCAACTGGATGGCACCCTTTATGTTTGATATTTCAAGACTTCTCACTTGCTGTA TTCCAGGTCTACCTGAAACTTTTTCCCGTGTTGGTCTAGGATATGTCACTTTGTTACCATGTCTAGTATT ACTCTTATCAAGGAATTGCAGGATCAGCCTTTCCGAGTGATTCTCATTGAGGGTGACCTCACAGAGAGTT ACCGACACCTGGGATTTAATAAGTCTGTAAATATTAAGACTAAGTTAGATAGTGGGGAGCTTTCAGAAGA TAGTGCAGAAGAACTGTGGACAAATCATGTGTTACAGGTGTTAATCCAGTTCAATGTGACCCTCATCTTG GTACAAGGAAGTGTATCAGAACACTTGACTGAAAAATGCATGCACAGTAAGCGGCTGGTAATTGGGGCAG TGAATGGCAGTGTGTTGCAGGCATTTGCAGAGGCCACAAGAGCAGTGCCAGTAGCCTATGTTACACAAGT GAATGAAGACTGTGTAGGCAGCGGGGTCTCTGTGACCTTCTGGATGAGTCCTCATGATATAAACAGGAGC AACAGAATAGCAATCTTGTTAACAGCAGAAGGAATTAATTTGATTACAGCAGTACTTACTAGTCCAGCAA GTGCTCAGATGGAAACCAAGGAAGACAGGTTCTGGTCTTGTGTGTATCGTTTATATCATGCCCTAAAAGA AGAGAAGGTCTTCCTTGGCGGTGGTGCTGTTGAATTTTTATGTCTTAGCCATCTTCAAATTCTTGCTGAG CAATCTTTAAATAGAGGAAACCATGCTTGTTTAGGATGGCTTCCTGATTCTTCCTCTTGGATGGCCTCAT CTCTGTCCGTCTACAGACCTACTGTGCTGAAGTCCCTGGCAGGTGGGTGGCATGAATTCCTGTCAGCTAT CATGTGTAACACTGCCACTCACCCATCAGCAGTGGAAGCTAGGACATTCATTCAACAACATGTACAAAAT GCCATTGACTCTGGCTCCCCTTCATCTTACATCTTGAGTGAATATAGTAAACTAAGTAGTGGAGTTTTTC ATTCAGGTATTTCAGATAACCTGGAGCTGGTTCCAAGAGTTTATGATACTGTTACACCAAAGATTGAAGC ATGGCGCCGAGCATTGGATGTAGTGCTCTTAGTGCTTCAGACAGACAGTGAAATAATTACTGGACTTGTA CACACAGAGATGAATTCACAGGAATTAGATGGAGTTTTATTTTTGTAGTATGTGTGCGCACACGTGTGGG TGTGCATGCATGTGTGTGCATGCACATGTACGTAGGTGCCTGATGAGGCCAGAAGAGGGGATTGGATCCC CTGGAGCTATAATTATAGGTAAGCCACCCAACAGTGTGTGCTGGAAACCCAGCTGTATAAGAACAGCAAG CACTTTTGAGTCATTTCTCCAGACTTAATAAATTTTAATAGTCATATCATAATGTGTAAATTACCAGCTA TTGTCAAGAATAAAAGAGTTGATGTCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30540467 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30540467 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12257 same embryo
	EMAGE:12254 same embryo
	EMAGE:12259 same embryo
	EMAGE:12256 same embryo
	EMAGE:12255 same embryo
	EurExpress:euxassay_007080 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source