EMAGE mouse gene expression spatial database

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EMAGE:12265

Ppp2r5b protein phosphatase 2, regulatory subunit B (B56), beta isoform ( MGI:2388480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007020. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007020_01	euxassay_007020_02	euxassay_007020_03	euxassay_007020_04

euxassay_007020_05	euxassay_007020_06	euxassay_007020_07	euxassay_007020_08	euxassay_007020_09

euxassay_007020_10	euxassay_007020_11	euxassay_007020_12	euxassay_007020_13	euxassay_007020_14

euxassay_007020_15	euxassay_007020_16	euxassay_007020_17	euxassay_007020_18	euxassay_007020_19

euxassay_007020_20	euxassay_007020_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12265_wholemount_strong.wlz

12265_wholemount_moderate.wlz

12265_wholemount_weak.wlz

12265_wholemount_possible.wlz

12265_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12265_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 08 09 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 18 19

trigeminal v nerve

strong

regional

strong expression: see section 10 17

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 17

cervical ganglion

strong

regional

strong expression: see section 09 17

thoracic ganglion

strong

regional

strong expression: see section 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

tongue

strong

spotted

strong expression: see section 15

stomach

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 11 12

midgut

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8411
Entity Detected:	Ppp2r5b, protein phosphatase 2, regulatory subunit B (B56), beta isoform ( MGI:2388480)
Sequence:	sense strand is shown >T8411 GCGCCGCCGCCCAGCCCCAGCGCCCCGAGCGGCAGAACCACCGCTAAGGACCCTTGAACCGTCGTCTCTT CCACCGAGGCAACCTCTAGGGTCGGCGACCAAAGTCTGGGTTTCTGAGAAAAGAGCCAGCGTGGGAACCC TGACTGGAACTCTTCTGGATCCCCAGGAAAGACCTGAGCCATTGCCTTTCTACCTTACCTGCCCCCCCAG GACTGGGCAGTTGCCAGAGGCCCTGGGGGGGTCAGGACTGTGGCCGTGCCTCCCCCACCCCCTGAAACAT GCTGGACAGGATGGGTCCCAGTTAGTGTGTGTGGCCTCATTCAACTTACTCCAGACCCACAAGGAGCCCC CTGGGCTCTGAAAGATTGACCCGTGTTGTCCACTGGCCATGGAGACGAAGCTGCCCCCTGCGAGCACCCC CACAAGCCCCTCCTCCCCAGGGCTGTCTCCAGTGCCACCACCAGACAAGGTGGATGGCTTCTCCCGCAGG TCCCTCCGCAGGGCCCGGCCCCGTCGCTCACACAGCTCTTCTCAGTTCCGCTATCAGAGCAACCAGCAAG AGCTCACTCCACTGCCCCTGCTCAAAGATGTGCCAGCCTCTGAGTTGCATGAGTTGCTGAGCCGGAAACT GGCCCAATGTGGGGTGATGTTTGACTTCTTGGACTGCGTGGCTGACCTCAAGGGGAAGGAGGTGAAGCGT GCAGCCCTCAATGAACTGGTGGAATGTGTGGGTTGCACCCGGGGTGTGCTCATCGAGCCCGTCTACCCAG ACATCATCCGCATGATATCAGTAAATATCTTCCGGACCCTGCCACCCAGCGAGAACCCTGAGTTTGACCC TGAAGAAGATGAGCCCAACCTTGAGCCCTCGTGGCCACATCTGCAGCTGGTATATGAGTTTTTCCTGCGT TTCTTGGAGAGTCCAGATTTCCAGCCCTCTGTGGCCAAGAGATACGTGGATCAAAAGTTTGTCCTAATGC TCCTGGAGCTATTTGACAGCGAGGACCCCCGGGAACGTGAGTACCTCAAGACCATCTTGCATCGGGTGTA CGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACATCTTCCTCCGGTTCATCTAC GAGCTGGAACACTTCAATGGTGTGGCTGAGCTCTTAGAGATCTTAGGAAGCATCATCAATGGCTTTGCGC TGCCCCTGAAGACTGAGCACAAGCAGTTCCTGGTTCGAGTCCTGATCCCCTTGCACTCTGTCAAGTCACT ATCTGTTTTTCATGCTCAGCTGGCATACTGTGTGGTGCAGTTCCTGGAGAAGGATGCAACCTTGACAGAG CATGTTATCCGGGGGCTTCTCAAATACTGGCCTAAAACCTGCACCCAGAAGGAGGTGATGTTCCTGGGGG AGATGGAAGAGATTCTTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATCCAGGAGCCCCTCTTCAAGCA GGTGGCTCGCTGTGTCTCCAGCCCCCATTTCCAGGTTGCAGAGCGGGCTCTGTATTTCTGGAACAATGAG TACATCCTGAGCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCGGTATTTGGGACCCTCTACCAAG TGTCCAAGGAGCACTGGAATCAAACCATCGTGTCCCTGATCTACAACGTGCTCAAGACTTTCATGGAGAT GAACGGAAAGCTGTTTGACGAGCTCACAGCCTCCTACAAGCTGGAAAAACAACAGGAGCAGCAGAAGGCC CAGGAGCGGCAGGAGCTATGGCGAGGCTTGGAGGAACTGCGGCTACGCCGGCTACAGGGGACCCAAGGGG CCAAGGAAGCCCCCGTCCCACGGCCTACGCCCCAGGTGGCTGCCAGTGGGGGTCAGAGCTAGATACACCT AGAACAGGAGATGCTAAACCCAGAGCTATCAGCCCCTCCATCCCTCTGCCCAGGGGCCCAGTGAGGCCCA CGCCTCCCCGTGGCCTTGCCGGAGTGGCTCTAGGACTCCCTACCAGCCCCGTGGGAACAGCTTTCACGGA GGGGGAAACAAGAAGGCAAGATGGTAGTCTTGGCAGCAGAACTCTCAGGCCTTTGTGGCAAGATTCTGGC AAGACTAGACCAGGGCAAGTATGCAACTGGGAAGCTGCCATCAGGGATCCTCCCCTGCCCTACACAGCCA GGCTCCAGGCGGCAGCTAGGCCTCTACCCCTGCTCCCAGCCTGGGGCATGGACACTCAGCGCCTGGCCAG GGCCTTCCTCATCCCCACCATGGGGGCACAGTCTATTTATTCTGCCCAGCTCACCCCCAACAAGACCTTG TCCAGGGACATTCTCCTATCCTCTCCCTGGCCCTGACTCCCTTATCCCCTTTTTATTTATTGGGCAGGGG GAGGGGTGAGGGCACAGGCAAGAAGATTCACATTGTCCTGGGGTGAGGGGGGAGGTCACAGTAATCATGG TCTGCCCCCTTCACCTGGCTGGGGGCAGATTTAATAAAGAGCGAAACTCAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5687054 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5687054 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12260 same embryo
	EMAGE:12261 same embryo
	EMAGE:12263 same embryo
	EMAGE:12264 same embryo
	EMAGE:12262 same embryo
	EurExpress:euxassay_007020 same experiment
	MGI:4827387 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source