EMAGE mouse gene expression spatial database

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EMAGE:12268

Gpr162 G protein-coupled receptor 162 ( MGI:1315214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007012. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007012_01	euxassay_007012_02	euxassay_007012_03	euxassay_007012_04

euxassay_007012_05	euxassay_007012_06	euxassay_007012_07	euxassay_007012_08	euxassay_007012_09

euxassay_007012_10	euxassay_007012_11	euxassay_007012_12	euxassay_007012_13	euxassay_007012_14

euxassay_007012_15	euxassay_007012_16	euxassay_007012_17	euxassay_007012_18	euxassay_007012_19

euxassay_007012_20	euxassay_007012_21	euxassay_007012_22	euxassay_007012_23	euxassay_007012_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12268_wholemount_strong.wlz

12268_wholemount_moderate.wlz

12268_wholemount_weak.wlz

12268_wholemount_possible.wlz

12268_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12268_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 17 18

trigeminal v nerve

strong

regional

strong expression: see section 08 09 16

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 10 11 12 16

cervical ganglion

strong

regional

strong expression: see section 08 09 17 moderate expression: see section 16

thoracic ganglion

strong

regional

strong expression: see section 13 14 moderate expression: see section 16 weak expression: see section 15

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

neural retina

strong

regional

strong expression: see section 01 02 21 22 23 24

stomach

moderate

spotted

moderate expression: see section 02 03 04 05 06 07 08 09 10

midgut

moderate

spotted

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4345
Entity Detected:	Gpr162, G protein-coupled receptor 162 ( MGI:1315214)
Sequence:	sense strand is shown >T4345 CAAGACTGGTCAGCGGCTCCCTAACCCCGGGCTCGCAGGCTGATGACCCTGGGAGACAGGGGCCTCGAGG GGCCGCAAACTAGCGCGCCGCGGGCTGAGCCCGAGAGGCCAGGCTGGGGGGAGGGAGGCCCGGAGGGAGG GAGTGAAGGAAGGAGGAGGGCTGGCGAGCTGGAGCCGGCAGGCGGCGGGAGCCCGGGCGAGCCTCGTCGG GAGTGCGTCTCCTCGGCAGAGCAGGACGCAGCCAGAGCTTGTCCAGGTGACTGAGCTCCACCCCTGTGCA CCAAGACACCCACCCCACCCCCCACTGGTCAAGCCTCGCTGGAGAAAGAGACCGGGAAGTCCCAGCATGA GAATCAGAGCTGCCCAGCCAGCTTCATAGATGGGGAAGCAGCCTGCTTGCCTCCCCCATCAATTACAGGG ATAACTGAGAAAAAGCCCCACCCAATATGGAGGCTGAGGACTACCGCCTCTATTAATTCCCTGCCTTCTC CCCTGCCCTGCCCACCTGGCCTTAGCTGGGTCACTCATGCAATGCTGAGAAGTAAGCAAGACTTGGGGTC AGCTTGCCTGGCTACCAGGCAAGGACTGTGGGCATCATGGGGGTGTGTGTGAGCGGGGCTCCAGGGTGAG ACCTAGCCCCCGCCCCCAGGAATTCAAGGGGGTGGGGGTGGGGCCGAGGATAGAATGGCTCGGGGAGGGT TGGGGGCAGAAGAGGCCTCCCTACGCTCAAACGCATTGTCCTGGCTGGCCTGTGGGCTCCTGGCCCTGCT GGCCAACGCTTGGATCATCCTTAGCATCTCAGCCAAACAGCAGAAACACAAGCCACTGGAGTTACTGCTC TGCTTCCTGGCCGGTACTCATATACTCATGGCGGCTGTGCCCCTTACCACCTTTGCTGTGGTGCAGCTCC GGCGCCAGGCTTCCTCTGACTATGACTGGAACGAGAGCATCTGCAAGGTCTTTGTGTCCACCTACTACAC GCTGGCCCTGGCCACCTGCTTCACGGTCGCCTCGCTCTCCTACCATCGCATGTGGATGGTGCGATGGCCC GTTAACTACCGCCTCAGCAACGCCAAGAAGCAGGCGCTGCATGCCGTCATGGGCATCTGGATGGTCAGCT TCATCCTCTCCACGCTGCCCTCCATCGGCTGGCACAACAACGGGGAGCGCTACTATGCCCGAGGCTGCCA GTTCATAGTCTCCAAGATCGGCCTCGGTTTTGGTGTCTGCTTCAGCCTCCTGCTTCTTGGGGGCATTGTC ATGGGGTTGGTCTGTGTGGCTATCACATTCTATCAGACACTGTGGGCCCGGCCCCGGAGGGCTCGGCAGG CCCGGAGAGCGGGGGGCAGTGTGGGCACCAAAGCAGGGGGGCTGGGGGGGTTGGGTACCCGGCCAGCTTT TGAGGTGCCAGCCATTGTGGTGGAGGATACTCGAGGGAAGCGGCGGTCCTCGCTGGATGGCTCTGAATCT GCCAAGACATCCTTGCAGGTCACCAACTTGGTCAGCGCCATCGTCTTTCTCTATGACTCACTCACGGGGG TGCCCATTTTGGTGGTGAGCTTCTTCTCCTTGAAGTCAGACTCAGCTCCGCCATGGATGGTGTTGGCAGT GCTGTGGTGCTCCATGGCACAGACACTGCTCCTGCCCTCCTTCATCTGGTCCTGTGAGCGCTACCGTGCA GATGTGCGCACAGTGTGGGAGCAGTGTGTGGCGATCATGTCCGAGGATGACGGTGATGACGATGGCACCT GTGACGACTACACAGATGGCCGAGTGTGCAAAATTCGCTTTGATGCTAACGGGGCCACAGGGTCAGGGAG TCGGGACCCCACCCAGGTGAAGCTGCTGCCTGGGCGGCACATGCTCTTCCCACCGCTGGAGAGAGTACAC TACTTACAGGTCCCTTTGTCCCGACGATTATCCCACGATGAGACCAACATCTTTTCTACTCCTCGGGCAC CAGGCTCCTTCCTGCACAAGTGGTCCTCATCGGATGATATCCGGATCCTCCCAGCCCAGAGCCGAGCCCT CGGAGGCCCTCCAGAATACCTGGGACAGAGACACAGGCTGGAGGATGAGGAGGATGAGGAAGAGGCTGAA GGCGGGGGCTTGGCCAGCCTTCGACAGTTTCTAGAAAGTGGGGTTCTGGGGTCGGGTGGGGGACCCCCAC GAGGTCCCGGTTTTTTCCGGGAGGAGATCACCACCTTCATCGATGAGACACCTCTGCCTTCTCCAACTGC GTCACCAGGACCCTCTCCTCGTAGGCCTAGACCACTGGGTTTTTCACCTCGACGGCTCTCCCTTGGGTCT CCGGATAGCCGAGCCGTTGGACTTCCTTTGGGGTTAAGTGCAGGAAGACGCTGTTCCCTGACCGGGAGCG AAGGGAGTTCAAGGGCTTGGGGAAGACCCTGGGGGCCAGGTAACCCCATCTTCCCCCAGTTGACCCTGTG AGGCCGCATAGGCCTGCTTCACTGGAGGGGGGGGGGAGGCACGAGCGAGTGAGTATGCCTCATCCTGGCC CTGAGTCTATGGCAGCTGCCTCCAGGGAGATGGGGGGGGGGGGGCCTACATCCGGGCTCTAGAGCCCCAG CTCTCTCCCATCCAAGTGACCAGATACCCAACGCACCCGTCTTCACCCCTAGCAAAATGTATTAAAAGGC TGAGCTGCCGTGGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527410 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527410 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12267 same embryo
	EMAGE:12269 same embryo
	EMAGE:12271 same embryo
	EMAGE:12270 same embryo
	EMAGE:12266 same embryo
	EurExpress:euxassay_007012 same experiment
	MGI:4825178 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source