EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12280

Ddr2 discoidin domain receptor family, member 2 ( MGI:1345277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010957. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010957_01	euxassay_010957_02	euxassay_010957_03	euxassay_010957_04

euxassay_010957_05	euxassay_010957_06	euxassay_010957_07	euxassay_010957_08	euxassay_010957_09

euxassay_010957_10	euxassay_010957_11	euxassay_010957_12	euxassay_010957_13	euxassay_010957_14

euxassay_010957_15	euxassay_010957_16	euxassay_010957_17	euxassay_010957_18	euxassay_010957_19

euxassay_010957_20	euxassay_010957_21	euxassay_010957_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12280_wholemount_strong.wlz

12280_wholemount_moderate.wlz

12280_wholemount_weak.wlz

12280_wholemount_possible.wlz

12280_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12280_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 12 13

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19

mandible

moderate

regional

moderate expression: see section 05 07 08 09 10 11 12 13 14 17 18 19 20 21 22

maxilla

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36272
Entity Detected:	Ddr2, discoidin domain receptor family, member 2 ( MGI:1345277)
Sequence:	sense strand is shown >T36272 GCTTGGTATCCTACAATGCTCCAGCTGGACAGCAGTTTGTACTCCCTGGAGGCTCCATCATTTATCTGAA TGATTCTGTCTATGATGGAGCTGTTGGGTACAGCATGACTGAAGGGCTAGGCCAGTTGACTGATGGAGTA TCCGGCCTGGATGATTTTACCCAGACCCATGAATACCACGTGTGGCCTGGCTATGACTACGTGGGATGGC GGAACGAAAGTGCTACCAACGGTTTCATTGAGATCATGTTTGAATTTGACCGAATCAGGAATTTTACTAC CATGAAGGTCCACTGCAACAACATGTTTGCTAAAGGTGTGAAGATTTTTAAGGAGGTCCAGTGCTACTTT CGCTCGGAAGCCAGCGAGTGGGAACCCACTGCTGTCTACTTTCCCCTGGTCCTGGACGATGTGAACCCCA GTGCCCGGTTTGTCACGGTGCCCCTCCACCACCGAATGGCCAGTGCCATCAAGTGCCAATACCATTTTGC CGACACGTGGATGATGTTCAGCGAGATCACTTTCCAATCAGATGCTGCAATGTATAACAACTCTGGAGCC CTTCCCACCTCTCCTATGGCACCCACCACCTATGATCCCATGCTTAAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64475. Forward Primer - name:064475_F_cDNA_Ddr2, sequence:GCTTGGTATCCTACAATGCTCC; Reverse Primer - name:064475_N_SP6_cDNA_Ddr2, sequence:CTTTAAGCATGGGATCATAGGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12282 same embryo
	EMAGE:12281 same embryo
	EurExpress:euxassay_010957 same experiment
	MGI:4824266 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source