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EMAGE:12281

Ddx4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 ( MGI:102670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281
"Pseudo-wholemount" of euxassay_010996. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010996_01 euxassay_010996_02 euxassay_010996_03 euxassay_010996_04
EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281
euxassay_010996_05 euxassay_010996_06 euxassay_010996_07 euxassay_010996_08 euxassay_010996_09
EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281
euxassay_010996_10 euxassay_010996_11 euxassay_010996_12 euxassay_010996_13 euxassay_010996_14
EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281 EMAGE:12281
euxassay_010996_15 euxassay_010996_16 euxassay_010996_17 euxassay_010996_18 euxassay_010996_19
EMAGE:12281 EMAGE:12281 EMAGE:12281
euxassay_010996_20 euxassay_010996_21 euxassay_010996_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12281Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12281_wholemount_strong.wlz
12281_wholemount_moderate.wlz
12281_wholemount_weak.wlz
12281_wholemount_possible.wlz
12281_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12281_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
ovary
strong strong
regionalstrong expression: see section 04 05 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36277
Entity Detected:Ddx4, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 ( MGI:102670)
Sequence:sense strand is shown

>T36277
TATATGGAGGAACCCAGTTTGGTCATTCAGTTCGACAGATAGTACAAGGGTGTAACATATTGTGTGCTAC
TCCAGGGAGGCTGATGGACATCATAGGCAAAGAAAAGATTGGCCTCAAACAAGTCAAGTACTTAGTTTTG
GATGAAGCTGATCGAATGTTGGATATGGGTTTTGGACCAGAGATGAAGAAACTAATTTCTTGTCCAGGAA
TGCCATCAAAGGAACAACGCCAAACCCTTTTATTCAGTGCTACTTTTCCAGAAGAAATCCAGAGGTTGGC
TGGGGACTTTCTAAAGTCCAGTTACTTGTTTGTCGCTGTTGGGCAAGTGGGAGGAGCTTGCAGAGATGTT
CAGCAGACGATCCTTCAAGTTGGCCAGTATTCAAAAAGAGAAAAGCTTGTTGAGATTCTACGAAACATAG
GTGATGAAAGAACTATGGTCTTTGTTGAAACCAAGAAAAAAGCCGATTTCATTGCAACTTTTCTTTGTCA
AGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAGCAGAGGGAGAGAGAGCAAGCTCTTGGAGAT
TTTCGCTGTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTGGCTGCCAGAGGGCTTGATATTGAAAATG
TTCAACATGTTATCAATTTTGACCTTCCTTCTACCATTGATGAGTATGTTCATCGAATTGGACGCACTGG
CCGCTGTGGAAATACTGGCAGAGCGATTTCTTTTTTTGATACTGACTCTGATAATCATTTAGCACAGCCT
CTAGTTAAAGTACTGTCAGACGCTCAACAGGATGTCCCCGCATGGCTAGAAGAGATTGCCTTCAGTACCT
ATGTGCCTCCCAGCTTCAGTAGCAGCACAAGAGGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89901. Forward Primer - name:089901_F_cDNA_Ddx4, sequence:TATATGGAGGAACCCAGTTTGG; Reverse Primer - name:089901_N_SP6_cDNA_Ddx4, sequence:CCCCTCTTGTGCTGCTACTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12280 same embryo
 EMAGE:12282 same embryo
 EurExpress:euxassay_010996 same experiment
 MGI:4824270 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS