EMAGE mouse gene expression spatial database

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EMAGE:12299

Cpne7 copine VII ( MGI:2142747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009558. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009558_01	euxassay_009558_02	euxassay_009558_03	euxassay_009558_04

euxassay_009558_05	euxassay_009558_06	euxassay_009558_07	euxassay_009558_08	euxassay_009558_09

euxassay_009558_10	euxassay_009558_11	euxassay_009558_12	euxassay_009558_13	euxassay_009558_14

euxassay_009558_15	euxassay_009558_16	euxassay_009558_17	euxassay_009558_18	euxassay_009558_19

euxassay_009558_20	euxassay_009558_21	euxassay_009558_22	euxassay_009558_23	euxassay_009558_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12299_wholemount_strong.wlz

12299_wholemount_moderate.wlz

12299_wholemount_weak.wlz

12299_wholemount_possible.wlz

12299_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12299_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 06 07 08 09 10 13 14 15 16 17

telencephalon mantle layer

strong

single cell

strong expression: see section 03 04 05 07 08 09 10 13 14 16 17 18 19 20 21

pons mantle layer

strong

single cell

strong expression: see section 06 07 08 11 15 16

midbrain mantle layer

strong

single cell

strong expression: see section 10 11 12 13 14 15

spinal cord mantle layer

strong

single cell

strong expression: see section 11

naris

strong

single cell

strong expression: see section 10 15

vomeronasal organ

strong

single cell

strong expression: see section 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36193
Entity Detected:	Cpne7, copine VII ( MGI:2142747)
Sequence:	sense strand is shown >T36193 CAGTGGCTACAGGTGGACAGAACAGAGGTGGTGAAGAGCAGCCTGCATCCTGTGTTCTCCAAGGTCTTCA CTGTCGACTACTACTTCGAGGGGGTGCAGAAGCTACGGTTTGAGGTGTATGACACCCACGGGCCTAGTGG GCTGACTTGCCAGGATGATGACTTCCTAGGGGGCATGGAGTGTACCCTGGGTCAAATTGTGGCCCAGAAG AAGATGACACGGCCATTGCTGTTGCGATTCGGCAGGAATGCTGGCAAGTCCACCATCACGGTGATAGCAG AGGACATCTCTGGGAACAACGGCTATGTGGAGCTCTCCTTCCAGGCCCGGAAACTGGATGACAAGGACCT CTTCAGCAAGTCGGACCCCTTCCTAGAGCTGTATCGGGTTAATGACGACGGGAGTGAGCAGCTCGTGTAC AGGACAGAGGTAGTGAAGAACAATCTTAACCCAGTGTGGGAACCCTTCAAGGTGTCCCTGAATTCACTCT GCAGCTGCGAGGAGACACGGCCTCTGAAGTGCCTGGTCTGGGACTATGACTCCCGAGGAAAGCATGACTT CATTGGCGACTTCACCACCACGTTCGCAGAGATGCAGAAGGCCTTTGAGGAGGAGCAGCAGGCCCAGTGG GACTGTGTGAATGCCAAGTACAAACAGAAGAAGCGCAATTACAAGAATTCCGGGGTGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66885. Forward Primer - name:066885_F_cDNA_Cpne7, sequence:CAGTGGCTACAGGTGGACAGA; Reverse Primer - name:066885_N_SP6_cDNA_Cpne7, sequence:ACCACCCCGGAATTCTTGTAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12300 same embryo
	EurExpress:euxassay_009558 same experiment
	MGI:4824032 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source