EMAGE mouse gene expression spatial database

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EMAGE:12300

St3gal1 ST3 beta-galactoside alpha-2,3-sialyltransferase 1 ( MGI:98304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010981. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010981_01	euxassay_010981_02	euxassay_010981_03	euxassay_010981_04

euxassay_010981_05	euxassay_010981_06	euxassay_010981_07	euxassay_010981_08	euxassay_010981_09

euxassay_010981_10	euxassay_010981_11	euxassay_010981_12	euxassay_010981_13	euxassay_010981_14

euxassay_010981_15	euxassay_010981_16	euxassay_010981_17	euxassay_010981_18	euxassay_010981_19

euxassay_010981_20	euxassay_010981_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12300_wholemount_strong.wlz

12300_wholemount_moderate.wlz

12300_wholemount_weak.wlz

12300_wholemount_possible.wlz

12300_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12300_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 04 19 weak expression: see section 05

cerebral cortex mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21

spinal cord mantle layer

moderate

single cell

moderate expression: see section 10 11 12 13 14 15 16

mandible

moderate

regional

moderate expression: see section 19 20 weak expression: see section 02 03 04 05 06 07 08 17 18

sternum

moderate

regional

moderate expression: see section 13 15 16 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9406
Entity Detected:	St3gal1, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 ( MGI:98304)
Sequence:	sense strand is shown >T9406 CACAAACCTAGGCGTGTCTCCAACAGGCTGAAGTTTGCCCTAGCTGCTTCTGAGATCTCCCTGCGGACTC CCTTTCCTGCTGCCTGCTCCTGTGTCTCTGAGATTTCTGTTCAGCTCTAGGAGGGGGGTCTCCTCTCTTC CACCCTCCCATCAACACAGAGGACCCAGCAAAGGGTGGAGGTGGTGAACATGAGGAGGAAGACCCTCAAG TACCTCACCTTCTTCCTGCTCTTCATCTTCCTCACTTCCTTTGTCCTGAATTACTCCAACACGGGAGTAC CCAGTGCCTGGTTCCCCAAGCAGATGCTCCTGGAACTCTCAGAGAACTTCCGCAGGTTCATCAAGTCACA GCCGTGCACCTGCAGACACTGCATCAGCCAGGACAAGGTATCATATTGGTTCGACCAGCGCTTCAACAAG ACTATGCAGCCCCTGCTGACAGTCCACAACGCTCTGATGGAGGAGGACACATACCGGTGGTGGCTGAGGC TCCAGCGGGAGAGGAAACCCAACAACCTGAGCGACACCGTCAAGGAACTGTTTCGCCTGGTGCCTGGCAA TGTGGATCCTATGTTGAACAAGAGGCTGGTGGGCTGCCGACGCTGTGCAGTTGTAGGAAACTCCGGTAAC CTGAAGGACTCCTCGTATGGGCCTGAGATCGACAGCCATGACTTTGTGCTGAGGATGAACAAGGCACCCA CAGTGGGTTTTGAGGCAGACGTTGGGAGCCGGACCACCCACCATCTCGTGTATCCTGAGAGCTTCCGGGA GCTGGGAGAGAATGTCAACATGGTCCTGGTCCCCTTCAAGACCACCGACCTGCAGTGGGTGATCAGCGCC ACCACCACAGGCACCATCACTCACACCTATGTCCCCGTGCCCCCGAAGATCAAAGTGAAACAAGAGAAGA TCCTGATCTACCACCCAGCCTTCATCAAGTATGTCTTTGACAACTGGCTTCAGGGCCATGGGCGGTATCC ATCGACTGGCATCCTCTCCATCATCTTCTCCATTCATATCTGTGATGAGGTGGACTTATATGGTTTTGGG GCAGACAGCAAAGGAAATTGGCACCATTACTGGGAGAACAACCCATCAGCGGGTGCCTTCCGAAAGACGG GGGTTCACGATGGTGACTTCGAGTACAACATCACAACTACCCTGGCAGCCATCAATAAAATCCGCATCTT CAAGGGGAGATGATGCTGCGACCCATTAAGGATGGACACAACACATCACACCTCTCAATTTGCAGCCCCA ACATCTCCCAGGAGCCAGTGTGTTCCAGACGCTTCCGGGGCTGAACTCGGGGTGTTCCCAGGCTTTCTGG CAGCCCCTTGCACCCTCATGTTGTTATCATCTACTTAGCAAAGTTGTCCAACTCTCCCAGGATACAGAGC CAGTCTCGGGCCTGTCTACAGTGGGAGCACTTTCCCACCGCTGTCCCCTAGCTGGGGAAAAGCGAGCCAA TACCAGGAAAGGTCTAGCCTCCAAACAAGTTATTTTGTCTTCTGAGTTGGATTGGAGGGAGGGGATGGGA GAAAATAAATCCTGAAGATTCTTATGTTCAAAAGAGTGAACATTTCCCGGAAGTCATTTAGAGGCTGCCA CTGAACTGTGGGCCTCATGCTCAACTCAGACTCTGGCTGGGAATGTTTGGGCCAGTCATCTGCAGAGCCA CACTGGGCTACACCAGCAGAGGGGCAAGATACCGTTCTCCTTTGCATGAAGCCTAGCCTGTTATTTGAGA TGACATCCCCATTGTCCCTTTCTTCAATAAGGTCACCGGTGATGGCTAGGCAAAACTTCTAACTCCAGAG GTCTTCCAGGGAAGCCTGGCCATGTGTGGCTGACAGGAGTGAAACCCTGGTCACAGTTCTGCGTGATTTT CCCTCCGTAGTGATGCTGATGGCCAGAGCAGGTCTGACTACTTCCACGTGCCTTTGAACTTGAGAAAGAA GTGCATGCATTGGCTGATAGGAAGCAAAACCCCCAATTGGAGAAAGGGGACCTCCACCAGCACCTCGCTT TGCTGCATAGCTCAAAATCTCTTTTCAAATGCTATTCCGCACCTCCACCCAGAATATACCAGGCATGGGA TGGGTCCTCACTTCTATTTCCTCAGACTGCTGGGTTCAGGACCACTAACAACCAGCATCCTCCCATGGGT CCTGAACACTGAGCCAAGTCCCCAGCAGCCACCTTCCTGGACTCGTTTGGGGCCGCACTGGGGACACTCT AGAACCACAACTGAAATGCATGTACCTCTTCTTCTTGGTTCTGTCTGCTCTCCCCTCCTTCCTCTATGGA AATCCTGATTATTTATTGATTTGTTAATTCAGCTGACCCTGTGCACACTCTTCCCCATGGCTGATCACAA TGTGTTTTGTTGCTTTGCCTGGCAAGTGGGAGCAGGAACAGAAGGGAGAGCATCCTGGATGTATGTGTTG AGACATCAGGACTTGGGGCTTGCTGGCGGGCAGATCCTGTGGCGCCGACAATCCTTTGTAGAATCCATGT CACCTCCCTTTCTAGTACAAGTGACTTGCCCTCTTCCTCACTGCAGCAGGGAGGAGAGCGGGCACTTGGA GGTAACTGTCACCTCCTTACAGATGTTCAATAAGAGAAACCTCTGTTTCTGTTGAAAAAATAGTCCCTAA AGACCACCACAACTTCATCAAGGCAGATTATGAGAGAGGGATCTTGAGGGTTGGGCCATCTCCCTCTACT CAGATTCCCCTCTGCAAGTAAGAGCTCGGGAGCCCCTCTCAACTTTGGGCTGTTCACAGACATCACCAAA GAGCAGACACTAAGACTGTCATTTCCTAGGAGTCTGTCCTGGGAGCTTTGGGATTGCTTTTGTTTAATTT TCGATGGCTCAGAATTCGGGCTTTTAATGAGGGAACGATAGGTTTCACCTTTTTTTTTTCCATTTTTTAT TAGGTATTTAGCTCATTTACATTTCCAATGCTATACCAAAAGTCCCCCATACCCACCCACCCCCACTCCC CTACCCTCCCACTCCCCCTTTTTGGCCCTGCCGTTCCCCTGTACTGGGGCATATAAAGTTTGCGTGTCCA ATGGGAAAATCTTCCTGCCATAAAACCCTTGGCTGAAAAGGCTAGACTCCTGTGAGGTCCTTGCTGTGGA GGGCCCTCCTCAAGGGTCCCAGTTTGAGGAACTCAGCAGATGTTTGTGGGTCAGCCCGTCAATCCCTACA TCAAATCAAAAATAGTGGACGTTGGCTTAGGGGTGAGAGTTCAGGTGTAGCTGGGTTCTGATGCATGAGA GCAAGTACCTGCAGTGAAGGTGTAGCCATTTCCAGCTGCTCAAGAAGTTCTTCTGCTCTCCATTGGCCCC TCCCACTCCCTCTGCCCCCACCTGCCTTCCTGCCCCCTCTCCCTCCCTCCCTCACTCCCTCCCTCCCTCC CTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCTTCCGGCCAAGGAGCCAATGGATCTCTTTCCTATAACCC TCATCCCTCCCCTGTGTTTCACTGGCTTAACGGTGGGCTCTTAACTGAATAGAATACAGTTGGTTGTGGC CAGAATTAAGTGCACCAATTAGTTTAAGGTAACTGGAGCTTTCCCTGGGTGTGAGTGACTGAGAACAGGA AAGGCCTTCCCCGAAGAGACTGGGCAGACTGCTGACAGAAAAGCTAGTCCTGGGTTCCAGGCAGAGAGCA AAGGGCTGGCTTCCAGTACAGATTCTTAGTAGAAGAATCTACTAAGTCCAAGGGAATTGGAGGTGGGGGT TCTCATCATCACCCCAGATGACTAACCAGGAGGCTGGTGTTTCTTCCACTCCCCTCAGCCTCTGTTAGGA GAGAGAGAAGCCGATCTGTAGCCTTTGGTTCCTGGGCATCAGACATCATTCAATAAGCCATGTGGGCAGG GTTAGTGGAACAGACCCCCAAAACAGTGATAGAACCAGCAACCGTCTAGGTCTGTGACAGTCTAAAGGTC TGGAGACCATAGAAGGATAGATACACAGTGGATATTGAGAACCTAGAGGGTGGCTTCACCTGTGCCCGTC AAAGAAGCCCCTGACATGCAGTGTGGGGGAGGCCCTAGATAACCCTGCACAGAGGACAGAGGGACAGCTG TATGCCCTCAGGAACTGGCAGTTGGAACCACAGGTTCTATCATAGCCAGGTGGCTATGAGGTGGGAAAGA TTGGATCTAGGCAGCTCAGCCACAAAGGCCTGGCTAGCCAATTTTCAGATAGCATCAGCTCTCCTCTTTG TCATCCTCTCCTGTGCCGCAGGTGTTACCTAAGACCTCTAAAAAGCACCAGAGGGTGAAGAAAGAAAGCA GGGACCAGCAGAGTAGTGCCCCGAGCTTTCAGCCTCAAAGTTAGTAAATGCGTCATCAGCCAAGCCCGGC CTGGAACCTGCTTCCGCCTGCTGAACCCCATCTTGACCTTCCTAACCTCTCTGCTCATTTAGCTTTTACC CTGGCTCCGCCTGCCCAGGGTCTGCCCCATGGCAACCGCTTTAACCCTTTCTCCAGAGCCTAATCCGGAT CTTGCACACCCTACCAGATGAACATGGGTAACTCCTGCTGCCCCCCACCTCCTTGCACAATCACTTTAAC CATTTCCCAAGAGCCTGATCTGGACTTTGCTCAACCCACCCTAGTAGGTAGACATGGGTTCCTCCTGCAC CTCTAGGCACAATCACTTTAGCCCTTTCCCAAGTCTGCTTCCGAGCCAAAGTTTTCCAAGCACTTTCTGC CCGAAAGCTCTTCCTTCCTTGCCTCCTGTGACCCAGCATTGCCTGTTTAAACTTTGCTTTCTCCTGCCCA CGTCTTGTAATCCTCCTGATTGGTCTGAATGCATTTCCAATGATGTCAGGGAGTCTGCCTTCCTCAGCCT CGGCTCCATTTTATTTATTGTTGAATATTTCCAATTATTCAAATCAAAGTGAATAAAAAAAGAGCTATTT TATTGTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30355523 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30355523 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12299 same embryo
	EurExpress:euxassay_010981 same experiment
	MGI:4828464 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source