EMAGE mouse gene expression spatial database

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EMAGE:12311

Rcan3 regulator of calcineurin 3 ( MGI:1858220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010962. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010962_01	euxassay_010962_02	euxassay_010962_03	euxassay_010962_04

euxassay_010962_05	euxassay_010962_06	euxassay_010962_07	euxassay_010962_08	euxassay_010962_09

euxassay_010962_10	euxassay_010962_11	euxassay_010962_12	euxassay_010962_13	euxassay_010962_14

euxassay_010962_15	euxassay_010962_16	euxassay_010962_17	euxassay_010962_18	euxassay_010962_19

euxassay_010962_20	euxassay_010962_21	euxassay_010962_22	euxassay_010962_23	euxassay_010962_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12311_wholemount_strong.wlz

12311_wholemount_moderate.wlz

12311_wholemount_weak.wlz

12311_wholemount_possible.wlz

12311_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12311_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9317
Entity Detected:	Rcan3, regulator of calcineurin 3 ( MGI:1858220)
Sequence:	sense strand is shown >T9317 CTTTACAGAGAAGATGCTCCGAGACAGCCTGAAATCTTGGAATGACAGCCAGTCAGACCTCTGTAGCAGC GACCAGGAGGAGGAAGAGGAGATGGTCTTCGGTGAAAATGAGGACGGACTGGAAGAGATGATGGACCTAA GTGACCTGCCCACCTCACTCTTTGCTTGCAGTGTCCATGAAGCAGTGTTTGAGGTCCAAGAGCAAAAGGA GAGGTTTGAGGCCCTGTTCACCCTCTACGATGACCAGGTCACATTCCAGTTGTTCAAGAGCTTTCGCAGA GTGAGGATCAACTTCAGCAAGCCCGAGGCTGCGGCAAGAGCGCGGATAGAGCTCCACGAGAGTGAGTTCC ACGGACGGAAGCTGAAGCTTTACTTCGCACAGGTGCAGGTGTCCGGGGAGGCCCGGGACAAGTCCTACTT ACTGCCACCACAACCCACCAAGCAGTTCCTCATCTCCCCTCCCGCCTCACCCCCCGTGGGGTGGAAGCAG AGTGAAGATGCGATGCCAGTGATCAACTATGACCTGCTCTGCGCTGTCTCCAAGCTGGGCCCAGGGGAGA AATACGAACTGCACGCGGGAACCGAGTCCACCCCCAGTGTGGTGGTGCACGTCTGTGAGAGCGAAACTGA AGAGGAAGAAGACACAAAAAATCCAAAACAGAAAATCACGCAGACGCGGCGCCCGGAGGCTCCCACGGCG GCACTGAGTGAGCGGCTGGACTGTGCACTCTGAGCGGCTGCGGTGCCTGCCGCGCCTGCCTGTCCCACCA CTACAGCTGCGCCTGTCTAGGAGCACAGCCCAGGGATGCTGTTGCATCCGTCAGGCAGTTGGGCCAGCCT CCCCAGGCCAGAACGTGGGGTGAAGTAGCAGATTCGGCCAGCTGTTTGAAATTCTGAACAGTATTCTCAA TGGCACTTTAAGTGACAAGGTTAGCCCTGACCATGTGGCACCTGTCTCCTCCCCCAGAAATATTAGTTCC CTTGAACCTGTAGAGCATTGGGTACCACAGGCCAGAACTAGAGTCACCCAGTGCACTGCCATTTTTACAG ATTTAAAAATTAGTCTTACGGTTTCAGTACTTTGGTGTGGGTATTTTTGTACAGGTGTGGCAGTGTCTTA ACAAATATATTGAGCAAAGGCTGAGTGTTGTTCAGTAGACATGACCTTTTTTTTGTTGTTGCATGAGAAG AAACCCGAGATACAAAATCATTTGTAGAAGTCATTTCTCACAAGTATTTTTTCACTAGCTTTGAGGATGA AAGGCCAAATGACTGAGATTGTCAGATCCAGATATCGTGGGCAGATGTGCTCTTAGCTCTCCTGGCTGTC CGTCTGTCCTGCCTCTCTTGGCAGTCAGTGACAGTGGTGTACAGGACTCTGCTCCCAGCTCAGCCCTTCC CAGCCTCGGCTCTGCTGGAGGAATCTCCCTTTGCCAGTGGGATGTGTCACCGGGAAGCTTGGCTGAGGAC TACAGAGTTGGAGTCTGTGGGGCAGCTCTTCAGTTACTCTCTTGACCCTCCTAATGCCGGCCTGCTACTG CTTGAGGTTTCCTGTGCTGTGCGTGTAATCAAGGACGTTAGTCGTCTTAACTTTAGAAGATAATGATAAG ACTTTGTGAAGACCGCTTTAAATAGGGAGAGCCAGTTTGCTTTTGTTTTCTTCAAAGACAGGGTTTCACT TTGTAGCTCCTGGGCCTCAAGTCCTCTGTGATCCTCCTGCCTCAGCTTCCCAAGTGCTGGAATTAGAGGG GTGAGCACCACACATTTCAAAGTTTTCCATTCAAGTATTTCAGCTGTTCAGAAAGAAAAATCACCTGTCC TTTCCCCCATGGATTAGGGAAAGTAATCATTCTTTTGGGGGTGGTTGGGTTTGTTTGTTTGGTTGGTTGG TTGGTTGGTTGGTTGATTTTGAGATAAGGTTTCACGCTGTAGCTCATGCTAGCCTGTCACTCATTATGTA GCCCAAGCTGGCCTTGAGGCCCAGACCACTCTCTTTCCTCAGGCTCTGTTTGTTTGGATTGTAGGCATGG GCTTGTTGTAGTCTATGCTTCCTCCTTTAACAATCATTTCCACCTTTGCTTGCAGTTGGCCCTCAGGTAG AATTGCTTCTTACACTTGAAGATGTGTTGTTGGGGCCTCCATAGGTCACTCAGCATCACTGGTCCCTTGC AGACTTTCCTGCCTTTGAGTGCTGGGCTACAGGTACATCAGCTGCTCCACTCATCAAGTATTCAGAGCTG GCTGGCCTAGGTGAAGGGGCTGGGCATAGAACAGAGAGGAACATAGCTGCAACTGCAAAGAAAAGAACGT AGAGAAATGAAGCCATGAGAGCCGTGTACTTTGCGTGACATGTTAGGGCTGAGTCCTTTTGTGGTAGGAT TGATTTCATGAGCGTGCATGCATGAGCTCACACACAAACACACGCACACAGGTGCATTGTCTGTCATTAA AACCGGGTCCCATGTAGTCCAGGTTGACCTCAAACTTGCTGTGTCCCAAGGATGACCCTGAACTGCTCCT CCTGCCTCTGTCTCATAAGTGCTAGGACTGAGGGTGTGTGCCACCATACCTGACAAGAACAGGAGAGTGT CCCTTGCCTTCAGGTCCTGTGGAATGCTGAATTCTGCGTTTGCCATTGGGAGCCCTCCGTGCAGATTCAG TTGCAGATTACGATTCCACCCGCATTGGTTTTTAGATCCAAGTCATCTGTCAGATAAAGTGAGTTTGGTT TTGAGGTGACAGCTCAGTGTGGCCCAGGTTGGTCTCAAACGCCAGGCCTCAGTGGGTCTTCCCAGTTGAC TGGATGCAGGCACACAGCTGCTGTAATCCTGAGACTTTGGAGGCGAATGCAGTAGATCAAGAGTTCATCC TTGGCTTACAGTGCTTTGCAGTGCTGAGCTCGGCAGTGTACTTCATTTGATTGTAAGGTAGACCCTAGCT GCCAGCAAGTGTGTTCTCACAGTTGGGGAGTTTGTGTGTTCCCCGTTATACAGTACACGTTCTCTCTAAT TTTCTATGTGATCATAACCCTGTTTTAAAATTTGTCTTGGCTTTCATTTTCTACCCCTCAATGAAAGAAC ATTCTTTTTTTTTTTAAGTCAAGAAGTTCAAATCTGCATACTTGATGCTACCTTGGCCACTGGCAGTTGT GTTGCCACCTTATCTGGGTTCCCTGGATAGTCACATTCATGCCATGTGTTAGAATTTACATGTTAAAATG TAAACAGTAGTGAGAGCGGAGCCAGGCACAAAGGATGCATGGAGTTAAAGGTTCTACGGAATGTCCCTCT CCCTCGGTTTAGAGAATCAAGTTCTATTCCAGACTAACGGTGGGCCTGGGCTTTATATTTTTATGTAAAC TATTTTTAGACTGGAGGATTTGGTTTTCAGAGGAAAGCTTTACGCATAATTAAACCATGGGAGTGTACTG GATTCTTTGATGCCTTGGGTTCACACCTACCTGCTTGCCTTCTTCTCTATAGAGCCTGAGACCTCACCCC GGGTCACCTCCAGCCTCCCTCTGTGCTCCATAATACATCTATCTTAAAGGAAACCGTTGTCTGGGTGTGG TGGCCCTGGCCTGTAATCTCAGCCTGCAGGAGACTGAGGCAGGAGGATTGAGAGAACGGGGCTAGCTTGG GCTACATGAGTTAAACCGTCTCAGAATAAGGTTTTTCCTTTTTCTTGGTGTGACAGTACAGAGCAACTGG GAAAATATTTACTTCTAGCCAAGAAGTCTGTTCTGGACTCATTCTACACAAACTTAAGTACACACACAGA GAGAGTACGATGTATGAACCTTGAGCAACCACATGACAAATGACTGTTGGTGTTTCAAACTCCCTTCAAG AGTGTGTTCTCCAGAAAAGCCATCCCTCTCAGAGTGCCACTGTTTCCCTCAGTCTTGAGTGGAGCGCAGT TCATAGTCAGGATTACCGTGTATTCAGCACAGTGACATAGGCTGTCCTCTCAGCGCTTGGGAGGCAGAGG TACCAGGGCTTGGCAAGCTTAAGGCCAGCCTGGGCCATGTACCGAGACCCTGTCTAAAAACAAGGGGATT CCTGGGTGTCCAGGTATCCCAGGGACAGATAACAGCAACTGTGCATCTTGTCCACGGCTGGGAACGTGCC GTATTCCTTCCCTGTGGTTTCTAGTCAAGGCTAGAAGTGATCCCAGATACGGTCAGCATGCAGACACCAC CTACTCACTCGGGTTAGTGCTTTCTCTTGGAAACCTGCAGTTCTTGAAGTGGGTCCCGAGACCCTTGCAG GTCCCACATGGCCTGACCTGACCGGATGTGCGTTTATGACACAGGGGGGTTTCTTTTCTATCTATGCAGA TAATTTGTGGTTTTCTGTACCGTTCCCTGTTGGCACCCCTCTTCCTAAGTCCAATTGAGGACTAAGGGAC CACAGGTCTCAGTCCTGTGCCGTGCTGTGCCCTCTGCTGTCAGCCTCTGAGTATCTATTCACGTCATGTG TGTCTTTGACGTGTGAGAACGCATTCACAGCTGTTGCACATTGATCCCCACAACTGAACCTGCAGCTGGA TTAAGTCCCGAATCCAGCATGTCTGTAATCCCCTGGTCTGTGCACTGTAGTATGCTCGCTGCACGTGTGG AAACTGGTCAGTGAGCCTCATCCGTGTGTCCTGTGATTTCAGTATCGTTGATCTGATGGGCCACAGTTCA TTATCAGCTTCAACTGTCCTGTGTCCTGAGGCTTTCGAACATTCCTTGTCCTGTAAGGAGGTGACAGAGG CAGACTGTGACCCGGAAGACACTTGTGATTACCTGTAACAAACGTTGATGTTGAAACTAATAAAGAATTG TTGTTTTTTAAGTCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6847734 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6847734 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12312 same embryo
	EMAGE:12314 same embryo
	EMAGE:12313 same embryo
	EurExpress:euxassay_010962 same experiment
	MGI:4827662 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source