EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12313

Mtmr1 myotubularin related protein 1 ( MGI:1858271)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010977. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010977_01	euxassay_010977_02	euxassay_010977_03	euxassay_010977_04

euxassay_010977_05	euxassay_010977_06	euxassay_010977_07	euxassay_010977_08	euxassay_010977_09

euxassay_010977_10	euxassay_010977_11	euxassay_010977_12	euxassay_010977_13	euxassay_010977_14

euxassay_010977_15	euxassay_010977_16	euxassay_010977_17	euxassay_010977_18	euxassay_010977_19

euxassay_010977_20	euxassay_010977_21	euxassay_010977_22	euxassay_010977_23	euxassay_010977_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12313_wholemount_strong.wlz

12313_wholemount_moderate.wlz

12313_wholemount_weak.wlz

12313_wholemount_possible.wlz

12313_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12313_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9336
Entity Detected:	Mtmr1, myotubularin related protein 1 ( MGI:1858271)
Sequence:	sense strand is shown >T9336 GGGTAACGCGGGGCCGCCAGGTGACAGCTAATGGCGGCGGCCGCTTGACGCGAGCGGTCAAGCAGCGAAG CAGGGCGGCAGGGGGCGCGTCGGGGAGCCTGGCTGCCGCTGCACTCCGGACTGTGGCTCACCGGAGGCGC CGGGCTTGACGCGTCATGGACAGGCCAGTGGCGGCGGCGGCCGCCGCATCTGCGGCGAGCTGCGAGGGCG CTGGGGGCCCGGGGCCAGGGCCGGGCGCTAGCTGGAGACCCTCTCGGGTTGCGGGGGGCGCCTCGGCCAG CTCCCGGCACCCCAGCATTGAGACCCTTGACAGTCCTACAGGGTCACATGTTGAATGGTGTAAACAGCTT ATAGCTGCTACAATTTCTAGTCAGATTTCAGGTTCAGTGACATCAGAAAATGTGTCAAGAGATTACAAGG CTCTAAGGGATGGAAATAAACTGGCACAGATGGAAGAAGCTCCACTTTTCCCAGGAGAATCAATTAAGGC CATTGTGAAAGACGTCATTTATATCTGTCCATTTATGGGAGCAGTGAGTGGGACCCTGACAGTGACGGAC TTTAAAATGTACTTCAAAAATGTAGAAAGGGACCCACATTTCGTCCTTGATGTTCCTCTTGGAGTGATCA GCAGAGTGGAGAAGATTGGTGCACAGAGCCATGGGGATAATTCTTGTGGTATAGAGATTGTGTGCAAGGA TATGAGGAACCTGAGACTTGCATATAAGCAGGAAGAACAGCGAAAACTTGGGATATTTGAAAACCTCAAC AAGCATGCTTTTCCTCTTTCCAATGGGCAGGTGCTATTTGCATTCAACTATAAAGAAAAGTTTCCAGTTA ATGGCTGGAAAGTTTATGATCCAGTGTCTGAATATAAGAGACAGGGCTTGCCAAATGAGAGTTGGAAAAT ATCAAAAATAAACAGTAATTATGAGTTCTGTGATACCTATCCTGCCATCATTGTTGTGCCAACTAGTGTG AAAGATGATGACCTTTCAAAAGTGGCAGCCTTTCGAGCGAAAGGCAGAGTCCCTGTGTTGTCATGGATTC ACCCAGAAAGTCAGGCAACAATTACACGTTGCAGCCAGCCATTAGTGGGTCCCAATGATAAACGCTGCAA AGAAGATGAGAAATACCTGCAGACAATAATGGATGCTAATGCACAGTCACATAAACTTACTATCTTTGAT GCCAGACAAAACAGCGTTGCTGATACCAACAAGGCAAAGGGTGGTGGATATGAAAATGAAAGTGCTTACC CTAATGCCGAACTCATATTCTTGGAGATCCACAATATTCATGTGATGAGGGAGTCACTTCGTAAATTAAA GGAGATTGTGTATCCTTCAATTGATGAGTCACATTGGCTATCCAATGTGGATGGGACACATTGGCTGGAA TATATACGGGTTCTCCTTGCTGGGGCAGTGAGAATTGCTGATAAAATAGAATCTGGGAAAACTTCCGTGG TCATCCACTGCAGTGATGGGTGGGATCGAACATCCCAGCTCACATCTCTGGCAATGCTGATGTTAGACAG TTATTACCGGACCATTAAAGGATTTGAGGCTCTCATAGAAAAGGAGTGGATAAGCTTTGGACACAGGTTT GCCCTGAGAGTGGGCCATGGTGATGACAACCATGCAGATGCTGACCGATCACCTATATTTCTTCAGTTTA TTGATTGTGTTTGGCAGATGACAAGACAGTTCCCTTCAGCATTTGAGTTTAATGAATTATTCTTGATTAC AATTTTGGATCACCTCTATAGTTGTCTTTTTGGGACCTTTTTGTGCAACTGTGAACAACAGCGAATCAAA GAGGATGTATATACAAATACTATATCTTTATGGTCTTATATCAATAGCCAACTAGATGAATTCTCAAATC CCTTCTTTGTGAATTATGAGAACCATGTGTTATATCCTGTTGCTAGTATGAGTCACTTGGAATTGTGGGT AAATTATTATGTACGGTGGAATCCACGGATGAGACCACAGATGCCAATTCATCAGAATCTCAAGGAACTG CTGGCTATCAAGGCTGAGCTGCAGAAGCGTGTGGAGGACTTGCAGCGGGAGATGGCCACCCGGACCATCT CATCTTCCTCTGAGAGGGGCTCCTCACCTACCCACTCAGCTACTCCTGTACACACCTCAGTCTGATGAAC AAAGCCAGTACCCCGGGCCATCCGGCTGCTCTATTGTCTTCCAGCAGCTCGGGGACCTGGCCATTGATAT TATGGTTCTAGCTTGTCATCTTAGCTCTTAGGACTCAGCCCAGGGACCATTTGTGGGTGGTGAAAGCTCC TATTATTAACAAGTACTTCTTTTGTGTGAACAGCCTGTGCCCCTTTTGCGAGCAGCCTCACCCTGTGAAC CACCTGTGTCTTGTACAGGTAGCTTGGGAGAAACCAGATTGAATGCAGTGCGGTATTTTAACTTAAGAGA ATCTTACACCACTTCTTTCCAAGTTACATCCTTTGTTACGAACAACTCGTTTACTTATAGAACACATCAC CAGCCATGTGGCTCCTCTCAGCCTTTTGGCACAATTTAAAAACTGTTTTAAATGAAATGTTAGTGTGGCA AATGAACCATTGCCACCTGTTCTACAGTTATCAGTTATCTTCATACAAGAAGTCCTGTTCAACTGGTACA ATATGTACTGCTGTGTACATATAAGGTATTTTTTAAAGAGAAACATGCCTTTATTTTCCTATGTCCTTTT TTATGTTTAGAATAGCCTGAGAAATCATCTGCTAAACTGTGCTGTAGGAGAAATTCTTTCCCAACGGAGT CCATGCTGCCTTTTGCGCAGCACATTTGAATTAAGATTCGAGATGTGTCAGCTCAGTGGCAATGGAGTGT TGTCCTCTAACTCTGGTACTTGTCTTTTGAGATGGCACAGCCCATTCATTCATTGGTGGTAGCAGCAGGT TCCCTCAGGATGCAGCAAACTATGAATGCTCAGTCCTCTGGCCTTGTCGCAGGTAAACTGAGGGCTCAGG CAGCAACTCTGCATTTGTTGGCCTCCCTCTATGACAGCTCTCTAAAGGAGCAAGTCTTGAAAATTGCTTT GGCATCCTACCACCTGTTAAGATGCCCAGCATCTATGTCAAATCAAGTCGTCTCTCACCTGTTAGACACC TGGACAGCTTGCTTTCATTTACCACGATCTCTTCTCAGTTGATTTACATGACTGCCTGGCCATACTTTAA GGCAACCAGTGGCTAAAATACTCATGAAATTAAATATCAGTGATTGCACTGAACTGTATACTTCAGATGA GCTAATTTTACTACGTTAATTTAGCATTTATTTAAGTGGAGAATTAATTAGTTTTGTATTCCCTATTTTA CAAAAATTGATAAAGATATAGTTCATGGATTTTATTCTGCTGTTATGGGTTTATTTCTATGGGTCTGAAA GCATAACATGCTCTTCCATGGTTTTCCCCTCTCGGACCCAGCCCTGGCTTGGCAGGCCTCTTTCCACAGT TAACAGTGTTGATCTCTGCTACTCAACCAGTCCTTCTAGGAATGAATCTCCCATCAGCTTCGACGTGTGC AATGCCAGCATAGCCACCACCACCACCATGTCCACTTCTGTGTGTCACCAGGCCCTTCAGAGTTGGAGAT GTGTTTGAGGCTGACCAAGTGAGCCCCTGAGGACAGATGGCTCACATCTTCCTCTTGTCAGTGTCAAATC ACTGGATGCTGTGCAGTCCTACACATTAACCTCGGTTTTCAAAAGTGTTCTTAAAAGAATTTTTACAGCC AAATACATAGAAACTAGATGATTTTTCAACTTACAGAAAACTACCCAACTATAAGATAACCAGAGACATT TATTCAAGGAAGTTTCATAAGTTATGATCAATTCTCCATTTTTAATCATCTGGGGGATTTGTTTCTAAAC TACAGTTTAAGTGATTTTAGCTTTTGATTTGTGTTCATCATAGATATAAAACCTTATTTGTAGATGGATA AACTGCCATATCTGAGGTAGGAGAAGCTGTGAGTTCAGGGACAGCTTTGGCTACATATCGAGTTCTGGGA CAGCTGGCCTATATTGTGGAGCCCTGTCTCAAAATCAATACCAAAAAGAAAATAACATGAACTATAATAT TTTTTTTCTTCATATACTTTTTAAAAGTGGGGACATTTACCAATTTTTCCCTAGAAATACTGTGTAATTT CCCAGCGACACTTGAACTATTGAAGATAGAGTGGACTGGATAATGTTTTGACAGAACCAGAGAAGCCATG GGGATGGCTGCTTGTTGAGGTCAGCTAGCCTGGTACAGATGGCTGGTAAACTGTGGCTCTGGCTCCTGCT CAAGCCATGGCCTCCCAGCTGCCATGCCTGCAGCACCCCTAAAAATGTCCACTGGATTTTAAAGAAGCAC AACAGAGTCTTTCCCTTTGTATGTTTCTAGCACAGAACTGTTTCTAAAACAACTTGAAGTATAGTTTTCT TATCTAAACAATTCTTTCATGTATGTTTTATTTAAGTACTATAATGTAAACCCTTTAAGGTTCAGGTTTT TAAACAAGTACAATACTGTATTTGCCTCATCTGAAGCACTGTATTCCAACATGTAACTAAAATGATAATA TGTTTGCTCCCTGGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6849757 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6849757 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12312 same embryo
	EMAGE:12311 same embryo
	EMAGE:12314 same embryo
	EurExpress:euxassay_010977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source